##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920947_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3589533
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.155286495485626	31.0	31.0	34.0	28.0	34.0
2	31.29411681129551	33.0	31.0	34.0	28.0	34.0
3	31.378510240747193	33.0	31.0	34.0	28.0	34.0
4	34.91765446925826	37.0	35.0	37.0	32.0	37.0
5	34.63170083684981	37.0	35.0	37.0	32.0	37.0
6	34.358918834288474	37.0	35.0	37.0	30.0	37.0
7	34.26277707991541	37.0	35.0	37.0	30.0	37.0
8	34.26412962354713	37.0	35.0	37.0	30.0	37.0
9	35.82079117255643	39.0	37.0	39.0	30.0	39.0
10-11	35.786750114847806	39.0	36.0	39.0	30.0	39.0
12-13	35.683434586058965	39.0	35.0	39.0	30.0	39.0
14-15	36.90346125805223	40.0	37.0	41.0	30.0	41.0
16-17	36.912429416305685	40.0	37.0	41.0	30.0	41.0
18-19	36.95348392116746	40.0	37.0	41.0	30.0	41.0
20-21	36.94879069227111	40.0	37.0	41.0	30.0	41.0
22-23	36.89946018047473	40.0	37.0	41.0	30.0	41.0
24-25	36.87782561129818	40.0	37.0	41.0	30.0	41.0
26-27	36.75175740131098	40.0	37.0	41.0	30.0	41.0
28-29	36.66026165520696	40.0	36.5	41.0	30.0	41.0
30-31	36.519475235357916	40.0	36.0	41.0	30.0	41.0
32-33	36.37124077143183	40.0	36.0	41.0	29.5	41.0
34-35	36.25481531998731	40.0	36.0	41.0	29.0	41.0
36-37	36.12911470656489	40.0	35.0	41.0	28.0	41.0
38-39	36.01060848305336	40.0	35.0	41.0	28.0	41.0
40-41	35.87665122454648	40.0	35.0	41.0	27.0	41.0
42-43	35.734494849329984	40.0	35.0	41.0	27.0	41.0
44-45	35.53339348043325	40.0	35.0	41.0	26.0	41.0
46-47	35.32404633137514	39.5	35.0	41.0	25.5	41.0
48-49	35.07607020188977	39.0	35.0	41.0	24.0	41.0
50-51	34.895961257355765	39.0	35.0	41.0	24.0	41.0
52-53	34.69827217078099	39.0	34.0	41.0	23.0	41.0
54-55	34.49158065965685	39.0	34.0	41.0	23.0	41.0
56-57	34.244093730298616	38.0	34.0	41.0	22.0	41.0
58-59	34.010737747779444	38.0	34.0	40.0	22.0	41.0
60-61	33.7438062277182	37.5	33.0	40.0	21.0	41.0
62-63	33.45209571830096	37.0	33.0	40.0	20.0	41.0
64-65	33.13674703645293	36.5	33.0	40.0	19.5	41.0
66-67	32.81045319822941	36.0	32.5	39.0	18.5	41.0
68-69	32.44317617361367	35.5	32.0	39.0	17.5	41.0
70-71	32.10710432248429	35.0	32.0	38.5	15.5	40.0
72-73	31.74455089283202	35.0	32.0	37.5	13.0	40.0
74-75	31.41428104993045	35.0	31.5	37.0	11.5	39.0
76-77	31.20367106250312	35.0	31.5	36.5	10.5	39.0
78-79	31.049636401169735	35.0	32.0	36.0	9.0	39.0
80-81	30.79962727742021	35.0	31.5	36.0	7.5	37.0
82-83	30.580219070280172	35.0	31.0	35.0	7.0	37.0
84-85	30.340746553938914	35.0	31.0	35.0	6.0	36.5
86-87	30.17096527598437	35.0	31.0	35.0	2.0	36.0
88-89	30.026571980254815	35.0	31.0	35.0	2.0	36.0
90-91	29.86743094435961	35.0	31.0	35.0	2.0	36.0
92-93	29.75357588299091	35.0	31.0	35.0	2.0	35.0
94-95	29.66502049152355	35.0	31.0	35.0	2.0	35.0
96-97	29.600276414787103	35.0	31.0	35.0	2.0	35.0
98-99	29.50172919429909	34.5	31.0	35.0	2.0	35.0
100-101	29.40879579599909	34.0	30.0	35.0	2.0	35.0
102-103	28.01584259010852	32.5	28.0	34.5	2.0	35.0
104-105	29.078829195887042	34.0	29.5	35.0	2.0	35.0
106-107	29.257206717419788	34.0	30.0	35.0	2.0	35.0
108-109	29.354080461163054	34.5	30.5	35.0	2.0	35.0
110-111	29.526179728672226	35.0	31.0	35.0	2.0	35.0
112-113	29.506651143756024	35.0	31.0	35.0	2.0	35.0
114-115	29.43064237046992	35.0	31.0	35.0	2.0	35.0
116-117	29.324365732255423	35.0	31.0	35.0	2.0	35.0
118-119	29.22827802391007	35.0	31.0	35.0	2.0	35.0
120-121	29.055308169614264	35.0	31.0	35.0	2.0	35.0
122-123	28.87687284111889	35.0	31.0	35.0	2.0	35.0
124-125	28.171985742992195	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63283.0
3	25424.0
4	10936.0
5	9104.0
6	10476.0
7	12137.0
8	13662.0
9	15240.0
10	17362.0
11	19770.0
12	21030.0
13	23945.0
14	28977.0
15	26206.0
16	17224.0
17	15117.0
18	13917.0
19	13287.0
20	13149.0
21	13710.0
22	14620.0
23	16777.0
24	22600.0
25	30385.0
26	38303.0
27	44722.0
28	53417.0
29	64501.0
30	76921.0
31	93954.0
32	116287.0
33	150003.0
34	202178.0
35	299594.0
36	568333.0
37	1014853.0
38	397229.0
39	900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.352661188533276	18.76996334603338	12.657581485908088	25.219793979525264
2	16.543897320081545	20.696713604036052	37.64721386161068	25.112175214271726
3	19.082800879784596	24.524120946701714	29.80578156101869	26.587296612495003
4	13.397508153023319	16.31471087899983	35.855611948964	34.43216901901285
5	13.647082768734908	37.66889160267885	34.43534581304961	14.248679815536633
6	33.05110871766054	36.160764060778774	16.617775607319498	14.17035161424118
7	28.307093054135535	32.1195061984352	21.25477941473574	18.318621332693525
8	26.85485823636718	34.879200616779144	19.664627732715292	18.601313414138385
9	26.752291757974312	14.864517337005237	19.32504833442322	39.05814257059724
10-11	25.5623358936524	25.581170510117197	27.686468275373237	21.170025320857164
12-13	25.994317192387665	23.091769430027995	27.88268886293974	23.031224514644595
14-15	23.31476351838038	24.40230004522404	25.32245467076869	26.960481765626888
16-17	23.10421279234642	27.36248121012049	26.097900897553306	23.435405099979782
18-19	23.18375011529158	26.239192894862164	28.075317515096575	22.501739474749684
20-21	23.898511249601032	25.49824119843425	27.826944904072903	22.776302647891814
22-23	23.66056433761735	25.332334737469452	27.707009075826555	23.300091849086645
24-25	23.33217241091081	25.57759605472124	27.8537098436934	23.236521690674543
26-27	23.164285237647654	25.743659355556286	27.892538755599727	23.199516651196326
28-29	23.219009418112133	25.779246941105733	27.971754022382573	23.02998961839956
30-31	23.38455091707276	25.7252488705937	27.73132855849878	23.158871653834755
32-33	23.16480763885938	25.809208230386172	27.699126571547783	23.326857559206662
34-35	23.313861903543213	25.821807509110993	27.863207830404214	23.00112275694158
36-37	23.554258898165056	25.579734212789152	27.636347323375553	23.22965956567024
38-39	23.355767290949846	25.81316478467748	27.6075052190787	23.22356270529398
40-41	23.382677691937946	25.7682881803426	27.707096531497953	23.1419375962215
42-43	23.38960423952355	25.696336616186	27.528648507669484	23.385410636620964
44-45	23.38351411277823	25.79879529906105	27.43976321155131	23.37792737660941
46-47	23.487545277267056	25.805189643116428	27.289296223493842	23.417968856122677
48-49	23.407585321985398	26.041717459782298	26.991380379252167	23.559316838980134
50-51	23.306919437618067	26.267950582610293	26.897662871758865	23.52746710801277
52-53	23.529079058321948	26.11706516683066	26.828148031952825	23.525707742894568
54-55	23.351855683101334	26.161345757727105	26.782607241897427	23.70419131727413
56-57	23.288281008362148	26.26925788357636	26.724118714106982	23.718342393954508
58-59	23.218764271326776	26.0508224324429	26.87765730537289	23.852755990857435
60-61	23.23999880741642	26.1650608120101	26.945325337079012	23.64961504349447
62-63	23.083171422062797	26.1294208046636	27.024174182856054	23.763233590417546
64-65	23.01437323831422	26.03832882126294	27.062976539834576	23.88432140058826
66-67	23.10546927518217	26.193854771720066	26.819205778446715	23.881470174651056
68-69	23.18128922846432	26.004129572332506	26.86802128118721	23.946559918015968
70-71	22.96422939584418	25.9318385947598	26.98304651213657	24.120885497259447
72-73	23.149206510205357	25.913714254505248	26.821353892658745	24.115725342630654
74-75	23.00043256782991	26.12178908939944	26.722995681983935	24.154782660786715
76-77	23.076419412599016	25.877400666437918	26.878144171426587	24.168035749536475
78-79	23.01821850136871	26.001616345006767	26.77622760790273	24.20393754572179
80-81	22.969574016164664	26.028570003558155	26.734168510526366	24.26768746975081
82-83	22.922033912472305	25.977458090032506	26.77912313488716	24.321384862608028
84-85	22.8870695446398	26.03675548438671	26.570322162876664	24.50585280809683
86-87	22.81481122353519	25.888197366325798	26.815699507974568	24.48129190216444
88-89	22.80449930731019	25.818633411611554	26.706661190534835	24.67020609054342
90-91	22.6870927224723	25.91012282827112	26.76049490175187	24.642289547504706
92-93	22.751905575617997	25.870691401373	26.763791095918084	24.613611927090922
94-95	22.679361926924518	25.749263873285898	26.80962626384208	24.761747935947504
96-97	22.73605262472327	25.51976707948417	26.84992945876406	24.8942508370285
98-99	22.619468207937874	25.661828461507596	26.700425625342707	25.018277705211823
100-101	22.530643224552865	25.625483170380665	26.834424398054512	25.009449207011958
102-103	22.39121448302872	25.490142807005668	26.905269546324885	25.21337316364073
104-105	22.3795496507775	25.63353605855693	26.757277755659313	25.22963653500625
106-107	22.27855401546284	25.66940168558891	26.746952705997074	25.30509159295117
108-109	22.2158137956428	25.749615250606396	26.748716817062686	25.28585413668812
110-111	22.15559194015367	25.78700577619813	26.70288347398273	25.35451880966547
112-113	22.111601487856383	25.823483038863497	26.699098306428702	25.36581716685141
114-115	22.015286001687794	25.966421836922382	26.5924079283492	25.42588423304062
116-117	21.862365943092406	25.94931075153498	26.66878030902547	25.519542996347138
118-119	21.979378979028507	25.869493994055905	26.52713066424772	25.623996362667867
120-121	21.85668062799803	26.04762918317575	26.56472963176965	25.530960557056563
122-123	21.69146023545242	26.06479085715241	26.538133388680368	25.705615518714797
124-125	21.64469710645904	26.12853780681051	26.514105538580175	25.71265954815028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93.0
1	117.5
2	273.0
3	485.0
4	381.0
5	225.5
6	418.5
7	984.0
8	1613.5
9	1767.0
10	1473.5
11	1199.5
12	1219.5
13	1385.5
14	1536.5
15	1692.0
16	1931.5
17	2177.5
18	2423.5
19	2772.0
20	3236.5
21	3755.5
22	4575.5
23	5709.5
24	6944.0
25	8581.0
26	11421.0
27	15375.5
28	18590.0
29	22552.5
30	28266.0
31	34333.0
32	41078.5
33	46961.5
34	52507.0
35	58763.5
36	65982.0
37	73654.5
38	80887.5
39	87562.0
40	93372.0
41	99568.0
42	106762.5
43	115879.5
44	123190.0
45	130335.5
46	138834.5
47	143560.5
48	147870.0
49	151181.5
50	154633.0
51	155653.5
52	150458.0
53	145929.0
54	141151.5
55	133485.0
56	124644.5
57	113083.0
58	102178.5
59	91609.0
60	77039.0
61	62972.0
62	50776.0
63	38138.5
64	27113.0
65	19847.5
66	14661.0
67	11023.5
68	8366.5
69	5953.0
70	4447.5
71	3342.0
72	2235.5
73	1638.0
74	1263.5
75	908.5
76	593.0
77	359.0
78	199.5
79	115.0
80	67.5
81	47.0
82	34.5
83	23.5
84	15.0
85	9.0
86	10.0
87	13.5
88	11.0
89	7.0
90	9.0
91	9.5
92	6.5
93	5.5
94	8.0
95	9.5
96	10.0
97	5.5
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21328679803194456
2	0.007856175162618648
3	5.01457989103318E-4
4	0.0018665380705512387
5	0.08115261790322029
6	0.05474249714377887
7	0.026911578748544728
8	0.015768067879582105
9	0.013260777934065519
10-11	0.0110599345374454
12-13	0.012870755053651827
14-15	0.020448342444546408
16-17	0.023192431996028454
18-19	0.02245417440095968
20-21	0.01625559648009922
22-23	0.013748306534582633
24-25	0.012062850515652035
26-27	0.011324592920583264
28-29	0.013678659591651616
30-31	0.010265959388031813
32-33	0.014361199632375575
34-35	0.01670133691485773
36-37	0.01802462883054704
38-39	0.02164626986295989
40-41	0.022509891955304492
42-43	0.020434413055960204
44-45	0.018818603979960626
46-47	0.01876288642561581
48-49	0.017495312064271313
50-51	0.014291552689444561
52-53	0.011965344795548612
54-55	0.017425665121340295
56-57	0.020699071439098066
58-59	0.01688241896647837
60-61	0.019111121140270894
62-63	0.02480824107202803
64-65	0.017857476167512598
66-67	0.020852294713546302
68-69	0.015085527838858147
70-71	0.01578199726816831
72-73	0.02309492627592503
74-75	0.013943317974789477
76-77	0.012578237893341558
78-79	0.015921291154030345
80-81	0.01654811364040949
82-83	0.012271791344445086
84-85	0.01593522054261655
86-87	0.01650632547465088
88-89	0.01741173573275409
90-91	0.012731461167789794
92-93	0.012815037499307012
94-95	0.013288636711237924
96-97	0.013859741643272257
98-99	0.012842896276479419
100-101	0.01692420713223698
102-103	0.022523821343890697
104-105	0.007633304945239395
106-107	0.007884033939791053
108-109	0.01416618819216873
110-111	0.011352451697755669
112-113	0.009541631181549243
114-115	0.006574671412687946
116-117	0.004248463518791999
118-119	0.002479431168344183
120-121	0.0026465838313786223
122-123	0.0016854560189305961
124-125	5.850343206205376E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3589533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.04540223868423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.83654316359106	31.69606318688099
2	14.4366783028608	13.294853188914216
3	6.088151934856468	8.40994214192069
4	3.2236476418686264	5.937366093825072
5	1.897247627815847	4.367976508458507
6	1.2326865648043794	3.405572922638372
7	0.8292692911714349	2.672882665332409
8	0.5954408216352729	2.1933849713223013
9	0.4470823694942597	1.8527478783468512
>10	2.2539153440694473	18.174160187817602
>50	0.11594333765214358	3.6115134966332474
>100	0.041522470182940065	3.203964966179562
>500	0.001267539678754781	0.3966831970859702
>1k	4.828722548597947E-4	0.42269702992452934
>5k	1.2071806371494859E-4	0.36019156471969876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7068	0.1969058370545695	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5892	0.1641439150998194	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4281	0.11926342507507244	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.785877717240655E-5	0.0
8	0.0	0.0	0.0	2.785877717240655E-5	0.0
9	0.0	0.0	0.0	2.785877717240655E-5	0.0
10-11	0.0	0.0	0.0	2.785877717240655E-5	0.0
12-13	0.0	0.0	0.0	2.785877717240655E-5	2.785877717240655E-5
14-15	0.0	0.0	0.0	5.57175543448131E-5	4.178816575860982E-5
16-17	0.0	0.0	0.0	5.57175543448131E-5	5.57175543448131E-5
18-19	0.0	0.0	0.0	9.750572010342292E-5	5.57175543448131E-5
20-21	0.0	0.0	0.0	1.5322327444823602E-4	5.57175543448131E-5
22-23	0.0	0.0	0.0	2.6465838313786223E-4	5.57175543448131E-5
24-25	0.0	0.0	0.0	3.343053260688786E-4	5.57175543448131E-5
26-27	0.0	0.0	0.0	6.825400407239605E-4	5.57175543448131E-5
28-29	0.0	0.0	0.0	0.0026465838313786223	5.57175543448131E-5
30-31	0.0	0.0	0.0	0.010781346765721334	5.57175543448131E-5
32-33	0.0	0.0	0.0	0.03050536100378517	5.57175543448131E-5
34-35	0.0	0.0	0.0	0.06314191846125944	5.57175543448131E-5
36-37	0.0	0.0	0.0	0.11943057773810689	5.57175543448131E-5
38-39	0.0	0.0	0.0	0.2139275499069099	5.57175543448131E-5
40-41	0.0	0.0	0.0	0.3230643094798126	5.57175543448131E-5
42-43	0.0	0.0	0.0	0.42969377910719864	5.57175543448131E-5
44-45	0.0	0.0	0.0	0.5598778448338544	5.57175543448131E-5
46-47	0.0	0.0	0.0	0.7179346171215031	5.57175543448131E-5
48-49	0.0	0.0	0.0	0.8886532036340103	5.57175543448131E-5
50-51	0.0	0.0	0.0	1.071267487999135	8.357633151721966E-5
52-53	0.0	0.0	0.0	1.2501347668345715	1.114351086896262E-4
54-55	2.785877717240655E-5	0.0	0.0	1.4466645103973135	1.114351086896262E-4
56-57	2.785877717240655E-5	0.0	0.0	1.6803439333194596	1.114351086896262E-4
58-59	2.785877717240655E-5	0.0	0.0	1.933496641485118	1.114351086896262E-4
60-61	2.785877717240655E-5	0.0	0.0	2.203322827788462	1.3929388586203276E-4
62-63	2.785877717240655E-5	0.0	0.0	2.4735390369722188	1.3929388586203276E-4
64-65	2.785877717240655E-5	0.0	0.0	2.764328953097799	1.671526630344393E-4
66-67	2.785877717240655E-5	0.0	0.0	3.0957091075635743	1.671526630344393E-4
68-69	2.785877717240655E-5	0.0	0.0	3.468097382027133	1.671526630344393E-4
70-71	2.785877717240655E-5	0.0	0.0	3.8697652312988904	1.671526630344393E-4
72-73	2.785877717240655E-5	0.0	0.0	4.270541599701131	1.9501144020684584E-4
74-75	2.785877717240655E-5	0.0	0.0	4.675134620575991	2.228702173792524E-4
76-77	2.785877717240655E-5	0.0	0.0	5.1284108545596325	2.228702173792524E-4
78-79	2.785877717240655E-5	0.0	0.0	5.631930393173708	2.228702173792524E-4
80-81	2.785877717240655E-5	0.0	0.0	6.174410431663395	2.228702173792524E-4
82-83	2.785877717240655E-5	0.0	0.0	6.729022967611664	2.228702173792524E-4
84-85	2.785877717240655E-5	0.0	0.0	7.279707415978624	2.228702173792524E-4
86-87	2.785877717240655E-5	0.0	0.0	7.8616494123330245	2.3679960596545565E-4
88-89	2.785877717240655E-5	0.0	0.0	8.499030375260514	2.5072899455165894E-4
90-91	2.785877717240655E-5	0.0	0.0	9.191975669258369	2.5072899455165894E-4
92-93	2.785877717240655E-5	0.0	0.0	9.898585693459289	2.5072899455165894E-4
94-95	2.785877717240655E-5	0.0	0.0	10.598342458475797	2.5072899455165894E-4
96-97	2.785877717240655E-5	0.0	0.0	11.317349638518436	2.7858777172406546E-4
98-99	2.785877717240655E-5	0.0	0.0	12.09229724312327	3.0644654889647204E-4
100-101	2.785877717240655E-5	0.0	0.0	12.92658404310533	3.0644654889647204E-4
102-103	2.785877717240655E-5	0.0	0.0	13.7819599374069	3.0644654889647204E-4
104-105	2.785877717240655E-5	0.0	0.0	14.624367570934716	3.0644654889647204E-4
106-107	2.785877717240655E-5	0.0	0.0	15.470800797763943	3.343053260688786E-4
108-109	2.785877717240655E-5	0.0	0.0	16.35744816944154	3.343053260688786E-4
110-111	2.785877717240655E-5	0.0	0.0	17.30174928047743	3.343053260688786E-4
112-113	2.785877717240655E-5	0.0	0.0	18.278282996701797	3.343053260688786E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8210	0.0	39.534916	1
GTATCAA	12165	0.0	38.3548	1
CATGGGG	9635	0.0	30.781536	4
TCAACGC	14725	0.0	30.616337	4
CAACGCA	14960	0.0	30.175209	5
AACGCAG	15385	0.0	29.48044	6
GTACATG	16035	0.0	29.470078	1
TATCAAC	15465	0.0	29.459831	2
ATCAACG	15380	0.0	29.428623	3
TACATGG	16260	0.0	28.422346	2
ACATGGG	16055	0.0	27.783321	3
ACGCAGA	17210	0.0	26.311226	7
CGCAGAG	17965	0.0	25.233665	8
AGTACTT	10780	0.0	22.879637	12-13
GCAGAGT	19750	0.0	22.563978	9
GAGTACT	10140	0.0	22.44589	12-13
ATGGGGA	5320	0.0	22.276869	5
AGAGTAC	17795	0.0	21.901688	10-11
GTACTTT	11040	0.0	20.616064	14-15
ATGGGGG	5330	0.0	20.22386	5
>>END_MODULE