FastQCFastQC Report
Mon 15 Aug 2016
SRR2920946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239138
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78400.029.7226981
CATGGGG77300.028.2799684
TACATGG123250.027.7907412
GTACATG128300.027.6387141
AACGCAG83300.027.299136
TCAACGC84950.026.9252784
CAACGCA86750.026.2979355
ACATGGG127550.026.2934253
ATCAACG88800.025.7595023
TATCAAC95800.023.8772872
ACGCAGA95400.023.7089317
GGTATCA58950.023.45481
CGCAGAG97450.023.1465978
ATGGGGA43500.021.9090275
GCAGAGT109000.020.092369
GTATAGC12600.019.3928151
ATGGGGG38400.019.3897025
AGAGTAC98400.017.65890710-11
GTGTAGC26050.017.3873481
ACTATAC9350.017.2016983
Produced by FastQC (version 0.11.5)