##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920946_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3239138
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.694088674209002	31.0	30.0	34.0	25.0	34.0
2	29.744105067459305	31.0	30.0	34.0	25.0	34.0
3	29.893007337137227	31.0	30.0	34.0	25.0	34.0
4	33.301146477859234	37.0	35.0	37.0	27.0	37.0
5	33.39114665691922	37.0	35.0	37.0	27.0	37.0
6	33.35567116930492	37.0	35.0	37.0	27.0	37.0
7	33.297713774467155	37.0	35.0	37.0	27.0	37.0
8	33.21911632045316	37.0	35.0	37.0	26.0	37.0
9	34.604505890147315	39.0	35.0	39.0	26.0	39.0
10-11	34.61787117436799	39.0	35.0	39.0	27.0	39.0
12-13	34.55959872657479	39.0	35.0	39.0	26.0	39.0
14-15	35.70805921204962	40.0	36.0	41.0	26.0	41.0
16-17	35.697923182031765	40.0	36.0	41.0	26.0	41.0
18-19	35.69196727647911	40.0	36.0	41.0	26.0	41.0
20-21	35.73319645535325	40.0	36.0	41.0	26.0	41.0
22-23	35.66085344310739	40.0	36.0	41.0	26.0	41.0
24-25	35.666799623850544	40.0	36.0	41.0	26.0	41.0
26-27	35.604264930978545	40.0	36.0	41.0	26.0	41.0
28-29	35.49860456701752	40.0	35.0	41.0	25.5	41.0
30-31	35.35845601514971	40.0	35.0	41.0	25.0	41.0
32-33	35.22959225571742	40.0	35.0	41.0	25.0	41.0
34-35	35.161069549985214	40.0	35.0	41.0	24.5	41.0
36-37	35.01609239865668	40.0	35.0	41.0	24.0	41.0
38-39	34.870223497733036	39.0	35.0	41.0	24.0	41.0
40-41	34.74479707255449	39.0	34.0	41.0	23.0	41.0
42-43	34.55707907474149	39.0	34.0	41.0	22.5	41.0
44-45	34.38990049204449	39.0	34.0	41.0	21.5	41.0
46-47	34.19011724724294	39.0	33.0	41.0	20.0	41.0
48-49	34.07104606225484	39.0	33.0	41.0	19.0	41.0
50-51	33.87670670406756	38.0	33.0	40.0	18.0	41.0
52-53	33.6859625925169	38.0	33.0	40.0	16.0	41.0
54-55	33.454539911544366	38.0	33.0	40.0	13.5	41.0
56-57	33.197024023057985	38.0	32.0	40.0	12.0	41.0
58-59	32.94198718918429	37.5	32.0	40.0	8.5	41.0
60-61	32.68554349953598	37.0	32.0	40.0	8.0	41.0
62-63	32.382036671484826	36.5	31.0	40.0	7.0	41.0
64-65	32.10469282259663	36.0	31.0	39.5	6.5	41.0
66-67	31.759706286055117	36.0	31.0	39.0	2.0	41.0
68-69	31.43199934673978	35.0	31.0	39.0	2.0	40.0
70-71	31.09938631821182	35.0	30.0	38.0	2.0	40.0
72-73	31.156602312096613	35.0	31.0	38.0	2.0	40.0
74-75	31.143499597732486	35.0	31.0	37.0	2.0	39.5
76-77	30.867025579027505	35.0	31.0	37.0	2.0	39.0
78-79	30.56386899848046	35.0	31.0	36.5	2.0	39.0
80-81	30.281342906662204	35.0	31.0	36.0	2.0	38.0
82-83	30.026032697588064	35.0	31.0	36.0	2.0	37.0
84-85	29.777464251291548	35.0	30.5	35.0	2.0	37.0
86-87	29.567335970248877	35.0	30.0	35.0	2.0	36.0
88-89	29.361249042183445	35.0	30.0	35.0	2.0	36.0
90-91	29.18308682742137	34.5	30.0	35.0	2.0	36.0
92-93	29.020161228079814	34.0	30.0	35.0	2.0	35.5
94-95	28.900927808571293	34.0	30.0	35.0	2.0	35.0
96-97	28.80293244684234	34.0	29.5	35.0	2.0	35.0
98-99	28.673384709141754	34.0	29.0	35.0	2.0	35.0
100-101	28.556850927623337	34.0	29.0	35.0	2.0	35.0
102-103	27.18457858232653	32.5	26.0	34.5	2.0	35.0
104-105	28.207385730401114	34.0	29.0	35.0	2.0	35.0
106-107	28.364982288497743	34.0	29.0	35.0	2.0	35.0
108-109	28.303331009669854	34.0	29.0	35.0	2.0	35.0
110-111	28.21128182868405	34.0	29.0	35.0	2.0	35.0
112-113	28.096480452515454	34.0	29.0	35.0	2.0	35.0
114-115	27.988428402865207	34.0	29.0	35.0	2.0	35.0
116-117	27.783382492502632	34.0	29.0	35.0	2.0	35.0
118-119	27.593887787429864	34.0	28.0	35.0	2.0	35.0
120-121	27.42291930754417	34.0	27.5	35.0	2.0	35.0
122-123	27.140348450729796	34.0	27.0	35.0	2.0	35.0
124-125	25.83022828913124	32.5	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124536.0
3	18013.0
4	11920.0
5	11744.0
6	15456.0
7	16385.0
8	15572.0
9	16145.0
10	17585.0
11	18885.0
12	19591.0
13	18927.0
14	19183.0
15	17634.0
16	14954.0
17	13840.0
18	13244.0
19	13034.0
20	13252.0
21	14417.0
22	16491.0
23	21000.0
24	28655.0
25	36433.0
26	40703.0
27	44592.0
28	51785.0
29	60974.0
30	72636.0
31	88940.0
32	111360.0
33	141619.0
34	192365.0
35	282273.0
36	499218.0
37	813086.0
38	312148.0
39	543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.170612302963015	18.395561507083592	12.145735465574774	25.28809072437862
2	15.782433784954966	20.95175402116816	38.94611243532784	24.31969975854903
3	18.855235412479825	24.81460381907782	29.531111115234804	26.799049653207547
4	12.631432489298186	16.83813787676644	36.86846934311196	33.661960290823416
5	13.434463861299637	38.31300671574744	34.431852095829676	13.820677327123237
6	32.115648813083666	36.91384753754074	17.31656923150625	13.653934417869346
7	27.545505373672114	33.13676384184563	21.431761488265945	17.885969296216313
8	25.16505454433079	36.37111777248988	20.15015915201335	18.31366853116598
9	25.907741052391493	16.653598900020615	19.58789713622753	37.85076291136036
10-11	24.472091875967873	26.99813713567576	27.97537996978195	20.55439101857441
12-13	24.894215115551543	24.68000362117224	27.660209836426215	22.765571426850002
14-15	22.484244812304944	26.13136568441796	25.166372990029824	26.21801651324727
16-17	22.608908303095617	28.5324521963952	26.109335915485993	22.749303585023185
18-19	22.52338608442626	27.87145153611129	27.460038119696094	22.14512425976636
20-21	23.689716226056486	27.158797307371362	26.912661959808293	22.23882450676386
22-23	23.20674545008024	26.961288419270684	26.740244876272147	23.091721254376928
24-25	22.786580395707706	27.044058090018552	27.130140603498198	23.039220910775544
26-27	22.786544223080245	27.178960201714926	26.976914689800896	23.05758088540394
28-29	22.8248902974046	27.490118000092373	26.922029479864417	22.76296222263861
30-31	23.03367940155715	27.30335249343978	26.833617861840864	22.829350243162203
32-33	22.63205711299337	27.437375283920495	26.82747628955866	23.103091313527475
34-35	22.917563604372653	27.48022488902689	26.84090236760659	22.76130913899387
36-37	23.02561716408388	27.23933524458702	26.7966201767956	22.9384274145335
38-39	22.834630100149283	27.397252444413688	26.822960434606376	22.945157020830656
40-41	22.971679910474148	27.36795044934402	26.88755539365762	22.772814246524213
42-43	22.86249416245815	27.305915926729757	26.820807123291818	23.010782787520274
44-45	22.892615550398286	27.368332109923948	26.82511980761578	22.913932532061978
46-47	22.746262358145085	27.36946443764377	26.78747852134149	23.096794682869653
48-49	22.659154597173075	27.341100253445077	26.885257447777633	23.114487701604215
50-51	22.65924950169849	27.4993697860609	26.774222149177817	23.06715856306279
52-53	22.85428961349803	27.364398382102728	26.62420342810714	23.157108576292103
54-55	22.689780867992045	27.335637091777603	26.670568566780805	23.304013473449547
56-57	22.729011993720217	27.383068526915466	26.598435367981104	23.289484111383214
58-59	22.619710491355967	27.14856736947607	26.846699181647455	23.385022957520516
60-61	22.726343794270452	27.132369217432434	26.86067000602283	23.28061698227429
62-63	22.503568509749243	27.130745161678476	26.97356236670703	23.392123961865256
64-65	22.474677111939677	27.09511953268112	26.880133696199483	23.55006965917972
66-67	22.609547075979762	27.26529201277837	26.66574607055288	23.45941484068899
68-69	22.79072131140957	26.968395658312954	26.79740106758086	23.44348196269661
70-71	22.642158161259704	26.891522756040693	26.884093283896192	23.582225798803407
72-73	22.67646193444955	26.87983961832126	26.89706087908744	23.546637568141747
74-75	22.56434264323537	26.988377694725397	26.872798720985745	23.574480941053487
76-77	22.531000312802906	26.78403855585463	26.977296687764774	23.70766444357769
78-79	22.575760711780138	26.919708489197163	26.939232335938673	23.56529846308402
80-81	22.67552989625499	26.79270876469034	26.961006536830045	23.570754802224634
82-83	22.64880368998891	26.76981236645516	26.944141821321114	23.637242122234824
84-85	22.63948211126563	26.743872978918333	26.952575008615465	23.664069901200573
86-87	22.48644514180568	26.71464341721902	26.98627346160874	23.812637979366556
88-89	22.701645637972003	26.548361467012484	26.90294652974023	23.847046365275286
90-91	22.63358785111011	26.526037009468382	26.99328537073956	23.847089768681943
92-93	22.730561159604022	26.489176129193886	26.973137327040327	23.807125384161772
94-95	22.48126819715463	26.544409282651536	27.08390003341117	23.89042248678266
96-97	22.556431040466205	26.3751048108185	27.10340968570114	23.965054463014155
98-99	22.41729314715089	26.54820321752222	26.87837578203236	24.156127853294528
100-101	22.414810829043425	26.679458659027716	26.94539392520391	23.96033658672495
102-103	22.394234489127893	26.58548846303236	27.030936416756308	23.98934063108344
104-105	22.222344056293107	26.704863192264412	26.86047447232162	24.21231827912086
106-107	22.107129298209465	26.85040827062722	26.85374449638591	24.188717934777404
108-109	22.18070626990933	26.78516707154631	26.854146947833545	24.179979710710818
110-111	22.15517712766828	26.99782419239786	26.723446296354076	24.123552383579774
112-113	22.22734706764446	27.04292368689824	26.73550504579305	23.994224199664252
114-115	22.091803846932727	27.149559121632322	26.687526192189697	24.071110839245257
116-117	22.075317690704015	27.154321742859295	26.67200836876961	24.098352197667076
118-119	22.13089470163251	27.067925257487126	26.635488683757664	24.165691357122697
120-121	22.2062538886771	27.20387202568334	26.530926220326347	24.058947865313215
122-123	22.223844092822898	27.228720517095596	26.446716298996325	24.10071909108518
124-125	22.261866914328845	27.270893174985254	26.318771748839158	24.14846816184674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2390.0
1	1696.5
2	820.5
3	577.0
4	464.5
5	412.5
6	373.5
7	350.5
8	422.5
9	481.0
10	485.0
11	524.5
12	560.0
13	613.0
14	719.0
15	829.0
16	998.5
17	1226.5
18	1494.0
19	1818.5
20	2258.0
21	2968.0
22	4013.0
23	5322.0
24	6990.0
25	9312.0
26	13023.5
27	17590.0
28	21676.0
29	26429.5
30	32059.5
31	38400.5
32	45750.0
33	52651.5
34	58648.5
35	64836.0
36	71754.5
37	78831.0
38	84546.5
39	88975.5
40	94829.5
41	101163.0
42	106672.5
43	113747.0
44	119687.0
45	123688.0
46	127815.0
47	129929.5
48	130789.0
49	131724.5
50	132955.5
51	130039.0
52	122493.5
53	118088.5
54	114152.5
55	106245.5
56	95862.0
57	85754.0
58	78915.0
59	70125.0
60	57743.0
61	47129.5
62	38715.5
63	29550.0
64	21614.5
65	16705.5
66	12401.0
67	9123.5
68	6868.5
69	5146.5
70	4024.5
71	3063.5
72	2364.0
73	2039.0
74	1655.0
75	1264.0
76	889.0
77	557.5
78	382.5
79	264.5
80	209.5
81	155.0
82	103.0
83	81.0
84	59.5
85	41.5
86	30.5
87	29.5
88	26.0
89	22.0
90	15.5
91	9.5
92	13.0
93	9.5
94	5.5
95	6.5
96	8.5
97	7.0
98	5.0
99	8.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.173132481542929
2	0.15254058332803355
3	0.17881300518841742
4	0.1504412593720922
5	0.17575663648785572
6	0.1127769178096148
7	0.11240644887621336
8	0.0999648671961491
9	0.1062319666528564
10-11	0.09238569026697843
12-13	0.08079309989262576
14-15	0.07980518273688865
16-17	0.0790951172812026
18-19	0.07690317609191087
20-21	0.07103741797972177
22-23	0.06152871535575206
24-25	0.06568105465095961
26-27	0.07690317609191087
28-29	0.0715776851742655
30-31	0.06804279410139365
32-33	0.07830787079772458
34-35	0.0750353952193454
36-37	0.07776760360318084
38-39	0.07316761434677992
40-41	0.07813807253658227
42-43	0.07855485008665886
44-45	0.07045084216850285
46-47	0.06804279410139365
48-49	0.06012402064993835
50-51	0.06612870461215299
52-53	0.06855218888482059
54-55	0.06957097845167448
56-57	0.07417096770807542
58-59	0.06061797922780691
60-61	0.07058976801852838
62-63	0.06614414081771137
64-65	0.060139456855496744
66-67	0.06498642540083195
68-69	0.0677186337846674
70-71	0.06268643077263147
72-73	0.05743812088277807
74-75	0.06043274476110619
76-77	0.07025017149624375
78-79	0.06393676342286127
80-81	0.062315961839230063
82-83	0.06679246145116385
84-85	0.06873742335152129
86-87	0.062192472194762934
88-89	0.06094213954453314
90-91	0.0648783719619232
92-93	0.05850321906630715
94-95	0.06781125101801776
96-97	0.05425826253774924
98-99	0.0537179953432055
100-101	0.05235960925406698
102-103	0.04972001810358188
104-105	0.048886463003428686
106-107	0.06010858444437995
108-109	0.06057167061113173
110-111	0.06727098382347403
112-113	0.06742534587905795
114-115	0.06938574398497378
116-117	0.07230318683550993
118-119	0.06580454429542675
120-121	0.06717836659012368
122-123	0.06591259773433548
124-125	0.07628572786957517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3239138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.39293662836611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.92786250440327	34.04549544608723
2	14.428792034093332	14.253608211276914
3	6.3575801016770335	9.420586532156852
4	3.426014046427007	6.768835787322451
5	1.9668993353758686	4.857546711329784
6	1.2396124338272707	3.6736859032659033
7	0.8617452632144582	2.9794890423022484
8	0.5579803244525181	2.204822944444671
9	0.42275778919321555	1.8793123821691786
>10	1.7067822013322878	13.828404417972246
>50	0.06919340869645192	2.371552359629453
>100	0.03258714531549666	2.782189443171996
>500	0.001754729593936564	0.586176219980941
>1k	4.3868239781980257E-4	0.3482945988900707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.087241111678477E-5
6	0.0	0.0	0.0	0.0	3.087241111678477E-5
7	0.0	0.0	0.0	3.087241111678477E-5	3.087241111678477E-5
8	0.0	0.0	0.0	3.087241111678477E-5	3.087241111678477E-5
9	0.0	0.0	0.0	1.234896444671391E-4	3.087241111678477E-5
10-11	0.0	0.0	0.0	1.234896444671391E-4	3.087241111678477E-5
12-13	0.0	0.0	0.0	1.8523446670070865E-4	3.087241111678477E-5
14-15	0.0	0.0	0.0	1.8523446670070865E-4	3.087241111678477E-5
16-17	0.0	0.0	0.0	1.8523446670070865E-4	3.087241111678477E-5
18-19	0.0	0.0	0.0	1.8523446670070865E-4	3.087241111678477E-5
20-21	0.0	0.0	0.0	2.1610687781749343E-4	3.087241111678477E-5
22-23	0.0	0.0	0.0	3.550327278430249E-4	3.087241111678477E-5
24-25	0.0	0.0	0.0	5.711396056605183E-4	3.087241111678477E-5
26-27	0.0	0.0	0.0	0.0010187895668538976	3.087241111678477E-5
28-29	0.0	0.0	0.0	0.0028093894116274143	3.087241111678477E-5
30-31	0.0	0.0	0.0	0.011283866263184835	3.087241111678477E-5
32-33	0.0	0.0	0.0	0.03442273839521502	3.087241111678477E-5
34-35	0.0	0.0	0.0	0.0750353952193454	3.087241111678477E-5
36-37	0.0	0.0	0.0	0.1435412754874908	3.087241111678477E-5
38-39	3.087241111678477E-5	0.0	0.0	0.25925107235320016	3.087241111678477E-5
40-41	3.087241111678477E-5	0.0	0.0	0.39203331256649143	3.087241111678477E-5
42-43	3.087241111678477E-5	0.0	0.0	0.5212034806791189	3.087241111678477E-5
44-45	3.087241111678477E-5	0.0	0.0	0.6791930445692651	3.087241111678477E-5
46-47	9.261723335035432E-5	0.0	0.0	0.8764677516055197	3.087241111678477E-5
48-49	1.234896444671391E-4	0.0	0.0	1.080055866715157	3.087241111678477E-5
50-51	1.234896444671391E-4	0.0	0.0	1.2956533497492235	3.087241111678477E-5
52-53	1.234896444671391E-4	0.0	0.0	1.507762250327093	6.174482223356954E-5
54-55	1.234896444671391E-4	0.0	0.0	1.7518704050275105	6.174482223356954E-5
56-57	1.234896444671391E-4	0.0	0.0	2.038798593946908	6.174482223356954E-5
58-59	1.234896444671391E-4	0.0	0.0	2.348865654998336	6.174482223356954E-5
60-61	1.234896444671391E-4	0.0	0.0	2.6774098541031597	6.174482223356954E-5
62-63	1.234896444671391E-4	0.0	0.0	3.0115265234145627	6.174482223356954E-5
64-65	1.234896444671391E-4	0.0	0.0	3.3667599219298467	6.174482223356954E-5
66-67	1.234896444671391E-4	0.0	0.0	3.776899903616332	6.174482223356954E-5
68-69	1.234896444671391E-4	0.0	0.0	4.231990115888857	6.174482223356954E-5
70-71	1.234896444671391E-4	0.0	0.0	4.734577532664554	6.174482223356954E-5
72-73	1.234896444671391E-4	0.0	0.0	5.232240799867125	7.718102779196193E-5
74-75	1.234896444671391E-4	0.0	0.0	5.742376521160876	9.261723335035432E-5
76-77	1.234896444671391E-4	0.0	0.0	6.312250357965607	9.261723335035432E-5
78-79	1.234896444671391E-4	1.5436205558392386E-5	0.0	6.9409670103589285	9.261723335035432E-5
80-81	1.234896444671391E-4	3.087241111678477E-5	0.0	7.602408418536043	9.261723335035432E-5
82-83	1.234896444671391E-4	3.087241111678477E-5	0.0	8.289875269284606	1.234896444671391E-4
84-85	1.234896444671391E-4	3.087241111678477E-5	0.0	8.974795146116033	1.234896444671391E-4
86-87	1.234896444671391E-4	3.087241111678477E-5	0.0	9.704186731161192	1.234896444671391E-4
88-89	1.234896444671391E-4	3.087241111678477E-5	0.0	10.48829966491085	1.234896444671391E-4
90-91	1.234896444671391E-4	3.087241111678477E-5	0.0	11.32809099210963	1.234896444671391E-4
92-93	1.234896444671391E-4	3.087241111678477E-5	0.0	12.181836649133196	1.234896444671391E-4
94-95	1.234896444671391E-4	3.087241111678477E-5	0.0	13.034764187262166	1.234896444671391E-4
96-97	1.234896444671391E-4	3.087241111678477E-5	0.0	13.912605761162384	1.234896444671391E-4
98-99	1.6979826114231624E-4	3.087241111678477E-5	0.0	14.8490277351567	1.234896444671391E-4
100-101	2.315430833758858E-4	3.087241111678477E-5	0.0	15.861642819787239	1.234896444671391E-4
102-103	2.77851700051063E-4	3.087241111678477E-5	0.0	16.884955194869747	1.234896444671391E-4
104-105	4.3221375563498685E-4	3.087241111678477E-5	0.0	17.89090183869906	1.234896444671391E-4
106-107	4.3221375563498685E-4	3.087241111678477E-5	0.0	18.90172632348483	1.234896444671391E-4
108-109	4.3221375563498685E-4	3.087241111678477E-5	0.0	19.952839304778	1.234896444671391E-4
110-111	4.476499611933792E-4	3.087241111678477E-5	0.0	21.05984678639811	1.234896444671391E-4
112-113	4.630861667517716E-4	3.087241111678477E-5	0.0	22.191181727978247	1.234896444671391E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7840	0.0	29.722698	1
CATGGGG	7730	0.0	28.279968	4
TACATGG	12325	0.0	27.790741	2
GTACATG	12830	0.0	27.638714	1
AACGCAG	8330	0.0	27.29913	6
TCAACGC	8495	0.0	26.925278	4
CAACGCA	8675	0.0	26.297935	5
ACATGGG	12755	0.0	26.293425	3
ATCAACG	8880	0.0	25.759502	3
TATCAAC	9580	0.0	23.877287	2
ACGCAGA	9540	0.0	23.708931	7
GGTATCA	5895	0.0	23.4548	1
CGCAGAG	9745	0.0	23.146597	8
ATGGGGA	4350	0.0	21.909027	5
GCAGAGT	10900	0.0	20.09236	9
GTATAGC	1260	0.0	19.392815	1
ATGGGGG	3840	0.0	19.389702	5
AGAGTAC	9840	0.0	17.658907	10-11
GTGTAGC	2605	0.0	17.387348	1
ACTATAC	935	0.0	17.201698	3
>>END_MODULE