FastQCFastQC Report
Mon 15 Aug 2016
SRR2920946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239138
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77870.24040346536640303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.19545323478036442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46190.14259966694842888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73800.045.3528021
GTATCAA117350.042.9098471
TCAACGC143500.034.525194
ATCAACG144900.034.2738043
CAACGCA144700.034.238875
TATCAAC146950.033.918292
AACGCAG147900.033.520726
ACGCAGA164100.030.0585787
CGCAGAG169550.028.9835198
CATGGGG74850.026.5716954
GTACATG128450.026.1964591
TACATGG126100.025.9732112
GCAGAGT187350.025.626869
ACATGGG126550.024.7507423
GAGTACT107300.024.37146812-13
AGTACTT113850.023.25677312-13
GTATCGA2603.052628E-822.8824319
AGAGTAC171800.022.70241410-11
CAGAGTA181250.022.0604210-11
GTACTTT120700.021.46858414-15
Produced by FastQC (version 0.11.5)