FastQCFastQC Report
Mon 15 Aug 2016
SRR2920945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4388819
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG109800.030.6981434
GTACATG168600.030.1427441
GTATCAA102400.028.8584541
TACATGG172300.028.5497742
ACATGGG173800.027.5837963
GGTATCA75750.027.0562211
TCAACGC114450.025.1841644
CAACGCA114850.024.9408955
AACGCAG115500.024.8894546
TATCAAC122900.023.8892732
ATCAACG121850.023.6063863
ACGCAGA129600.021.8576097
CGCAGAG135200.020.8635168
TAATGCG2055.901866E-520.3349064
ATGGGGA62300.019.7870145
ATGGGGG55400.019.6715835
GTATAGG11750.019.2680191
ATGGGGC46850.018.6855435
ACCGTAT6300.017.947298
AGAGTAC127350.017.6147410-11
Produced by FastQC (version 0.11.5)