FastQCFastQC Report
Mon 15 Aug 2016
SRR2920945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4388819
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.17489898763198025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.15418726541240366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45660.10403709973001848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87950.043.3440061
GTATCAA132300.041.485091
TCAACGC163550.032.8766984
CAACGCA164400.032.4893955
TATCAAC167450.032.2906272
ATCAACG169050.031.771843
AACGCAG171250.031.1748876
GTACATG159450.029.5948371
CATGGGG102000.028.780464
TACATGG163300.028.2247772
ACGCAGA194200.027.270297
CGCAGAG195500.026.84248
ACATGGG166800.026.8098813
GAGTACT120750.023.42949312-13
GCAGAGT220400.023.350399
AGAGTAC192750.022.34825910-11
AGTACTT131350.022.01434112-13
CAGAGTA207150.021.11066610-11
GTACTTT135700.020.71674214-15
GTACCGT6650.020.584926
Produced by FastQC (version 0.11.5)