FastQCFastQC Report
Mon 15 Aug 2016
SRR2920944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242865
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32620.1454389809462451No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23130.1031270272620064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116200.028.5105171
CATGGGG69800.027.903834
GTATCAA76550.027.0875971
TACATGG118500.026.9915282
ACATGGG120350.025.685783
GGTATCA59650.024.8728371
AACGCAG83850.023.7101176
CAACGCA85800.023.3945185
TCAACGC85650.023.3659484
TATCAAC90900.022.4751052
ATCAACG90350.022.2163773
ATGGGGA33200.021.8873565
ACGCAGA94800.020.5937967
GTATAGG4650.020.5023421
CGCAGAG97400.019.9816178
TAGTACA4555.456968E-1219.6359254
ATGGGGG30650.018.850065
GCAGAGT105600.018.0354869
GAGTACT28300.017.34594212-13
CTATAGT6400.016.7518984
Produced by FastQC (version 0.11.5)