##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920944_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2242865 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.760260648768426 31.0 30.0 34.0 25.0 34.0 2 29.788670740325433 31.0 30.0 34.0 25.0 34.0 3 29.932376224159725 31.0 31.0 34.0 25.0 34.0 4 33.318614361542046 37.0 35.0 37.0 27.0 37.0 5 33.424841441638264 37.0 35.0 37.0 27.0 37.0 6 33.39191346781906 37.0 35.0 37.0 27.0 37.0 7 33.33789684176266 37.0 35.0 37.0 27.0 37.0 8 33.24188214627274 37.0 35.0 37.0 26.0 37.0 9 34.635962931340046 39.0 35.0 39.0 26.0 39.0 10-11 34.65243628127418 39.0 35.0 39.0 27.0 39.0 12-13 34.59324480073478 39.0 35.0 39.0 26.5 39.0 14-15 35.75669868672435 40.0 36.0 41.0 26.5 41.0 16-17 35.74017183379294 40.0 36.0 41.0 26.5 41.0 18-19 35.71632755426653 40.0 36.0 41.0 26.0 41.0 20-21 35.75133634882171 40.0 36.0 41.0 26.0 41.0 22-23 35.68193515882588 40.0 36.0 41.0 26.0 41.0 24-25 35.684059227818 40.0 36.0 41.0 26.0 41.0 26-27 35.60983072989235 40.0 35.5 41.0 26.0 41.0 28-29 35.50161512173047 40.0 35.0 41.0 25.5 41.0 30-31 35.352971088317844 40.0 35.0 41.0 25.0 41.0 32-33 35.21388469658227 40.0 35.0 41.0 25.0 41.0 34-35 35.13783798846565 40.0 35.0 41.0 24.5 41.0 36-37 34.9917106022877 40.0 35.0 41.0 24.0 41.0 38-39 34.838374133084244 39.0 35.0 41.0 23.5 41.0 40-41 34.68902452889496 39.0 34.0 41.0 23.0 41.0 42-43 34.48595234220517 39.0 34.0 41.0 21.5 41.0 44-45 34.30949655908849 39.0 34.0 41.0 20.5 41.0 46-47 34.091115604372085 39.0 33.0 41.0 19.0 41.0 48-49 33.94609773660029 38.0 33.0 40.5 18.5 41.0 50-51 33.726834651216194 38.0 33.0 40.0 16.5 41.0 52-53 33.511794512821766 38.0 33.0 40.0 14.5 41.0 54-55 33.251869818290444 38.0 32.5 40.0 13.0 41.0 56-57 32.963317230417346 37.5 32.0 40.0 9.0 41.0 58-59 32.67022625079976 37.0 32.0 40.0 8.0 41.0 60-61 32.370864719900666 36.5 31.0 40.0 7.0 41.0 62-63 32.03639942662621 36.0 31.0 39.0 6.0 41.0 64-65 31.723301447033148 35.5 31.0 39.0 2.0 41.0 66-67 31.35520439259608 35.0 31.0 39.0 2.0 40.0 68-69 31.008117965191843 35.0 30.0 38.0 2.0 40.0 70-71 30.652634465293275 35.0 30.0 37.0 2.0 40.0 72-73 30.716276057631646 35.0 30.5 37.0 2.0 39.0 74-75 30.71536895889855 35.0 31.0 37.0 2.0 39.0 76-77 30.46618588278831 35.0 31.0 36.0 2.0 39.0 78-79 30.19660456603496 35.0 31.0 36.0 2.0 37.5 80-81 29.959714472337836 35.0 31.0 35.0 2.0 37.0 82-83 29.75028925057906 35.0 30.0 35.0 2.0 37.0 84-85 29.54473742289438 35.0 30.0 35.0 2.0 36.0 86-87 29.378176350337625 35.0 30.0 35.0 2.0 36.0 88-89 29.202335628760537 35.0 30.0 35.0 2.0 36.0 90-91 29.051466762377586 34.0 30.0 35.0 2.0 35.0 92-93 28.89929242286094 34.0 30.0 35.0 2.0 35.0 94-95 28.79551399660702 34.0 30.0 35.0 2.0 35.0 96-97 28.709212770273737 34.0 29.0 35.0 2.0 35.0 98-99 28.57560798353891 34.0 29.0 35.0 2.0 35.0 100-101 28.447189420674004 34.0 29.0 35.0 2.0 35.0 102-103 27.074665661999276 32.5 26.0 34.5 2.0 35.0 104-105 28.08507912870369 34.0 29.0 35.0 2.0 35.0 106-107 28.236186752212014 34.0 29.0 35.0 2.0 35.0 108-109 28.159125939367726 34.0 29.0 35.0 2.0 35.0 110-111 28.044475035278538 34.0 29.0 35.0 2.0 35.0 112-113 27.909049140273712 34.0 29.0 35.0 2.0 35.0 114-115 27.780207457871963 34.0 29.0 35.0 2.0 35.0 116-117 27.55772795063457 34.0 27.5 35.0 2.0 35.0 118-119 27.33958954283918 34.0 27.0 35.0 2.0 35.0 120-121 27.137115920931485 34.0 27.0 35.0 2.0 35.0 122-123 26.818724042686476 34.0 26.0 35.0 2.0 35.0 124-125 25.504940555940728 32.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84802.0 3 12992.0 4 8837.0 5 8804.0 6 11737.0 7 12037.0 8 11176.0 9 11561.0 10 12503.0 11 13725.0 12 14008.0 13 13860.0 14 13497.0 15 11846.0 16 9574.0 17 8657.0 18 8398.0 19 8492.0 20 8912.0 21 9772.0 22 11745.0 23 15116.0 24 21037.0 25 26401.0 26 28969.0 27 31992.0 28 37471.0 29 43707.0 30 52124.0 31 64142.0 32 79620.0 33 102659.0 34 139178.0 35 206526.0 36 375041.0 37 548658.0 38 163068.0 39 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13786944908046 17.80822835491407 11.64680982765035 25.407092368355123 2 16.51678148437235 20.781498661303548 37.30814845509607 25.393571399228037 3 19.357123810859495 24.22164029144578 28.724685750681022 27.6965501470137 4 13.239941255242755 16.148914119505125 34.876506964218656 35.73463766103346 5 13.99020428435267 38.04535900502828 33.3985577090567 14.56587900156235 6 33.55471065467816 35.48042107368075 16.44421809274191 14.52065017889918 7 28.80069452503593 32.64772047599068 20.326239298690403 18.22534570028299 8 26.687318356766948 35.21232174863076 19.34960118822345 18.750758706378846 9 26.612199171606083 16.09065914446904 18.48755623794901 38.80958544597586 10-11 25.504352313963707 26.28125558528922 26.84725387296924 21.36713822777783 12-13 25.571376300712156 23.947003230585253 26.842593749442234 23.639026719260357 14-15 23.397059479719783 25.20411851323011 24.011065994377763 27.387756012672348 16-17 23.47204066957731 27.819308991111676 25.010781605610315 23.6978687337007 18-19 23.60655957147048 26.968579782054096 26.390395120367753 23.03446552610766 20-21 24.569570448295472 26.16968395493911 25.922079741540376 23.338665855225045 22-23 24.322170802295116 25.890525671471725 25.76614229048338 24.021161235749783 24-25 23.88153993797524 26.122988556516724 25.945864190673262 24.049607314834766 26-27 23.67713998934024 26.191085468390284 26.012693994592478 24.119080547676997 28-29 23.819274958170663 26.33122141662019 26.073909648633574 23.77559397657557 30-31 23.999302198009346 26.331071797533912 25.73296536622223 23.936660638234507 32-33 23.61243609227878 26.54299944402848 25.741191242600802 24.103373221091935 34-35 23.910320708346198 26.36522806898558 25.916844996617826 23.807606226050392 36-37 24.085307289229966 26.176849403300984 25.75708188958073 23.98076141788832 38-39 23.955175416306837 26.401069414925004 25.73623225329178 23.907522915476378 40-41 23.89454036938937 26.386660324554327 25.887642876011107 23.83115643004519 42-43 23.88484165515635 26.29662012787885 25.801373473939538 24.017164743025262 44-45 23.791404826594928 26.406663723394264 25.781393089943 24.020538360067807 46-47 23.80931985035771 26.513923559291406 25.740414795580328 23.936341794770552 48-49 23.670740999825117 26.58397545942203 25.627567463390783 24.11771607736207 50-51 23.554830704819928 26.704272946009628 25.69920062675645 24.041695722413994 52-53 23.814681254747466 26.453586100201775 25.535514109383833 24.196218535666922 54-55 23.610918080044474 26.57300489472253 25.50524998644755 24.31082703878545 56-57 23.651134950533173 26.5911460785053 25.493831649933806 24.26388732102772 58-59 23.592791771125103 26.302000672311195 25.71837923481885 24.38682832174485 60-61 23.66062734171172 26.38757050929546 25.75674864725888 24.19505350173394 62-63 23.537261393016404 26.384617615369 25.851422886193642 24.226698105420954 64-65 23.535049969094846 26.447517784126106 25.69082666770167 24.32660557907738 66-67 23.506193923924922 26.59591720555244 25.71382427427484 24.184064596247797 68-69 23.752257549853482 26.325418226725823 25.739012959942322 24.183311263478377 70-71 23.472121252814286 26.272119735949538 25.866648404804803 24.389110606431373 72-73 23.55734088865602 26.337142891557075 25.791951591687805 24.313564628099098 74-75 23.43774955275685 26.54743900317197 25.80303010024492 24.21178134382626 76-77 23.536683006116753 26.31859094495681 25.919515139292827 24.225210909633613 78-79 23.572114035467333 26.365024673837347 25.823520830108105 24.23934046058722 80-81 23.596535234875002 26.343961655683223 25.922231276261954 24.137271833179827 82-83 23.445873083929435 26.28353360510152 25.98855789603774 24.282035414931308 84-85 23.466040848468488 26.340157027125606 25.842997413369055 24.35080471103685 86-87 23.454483725960124 26.232509355409185 25.90395470143887 24.409052217191824 88-89 23.44708377267817 26.170326529037137 25.88656571791976 24.49602398036494 90-91 23.406343174210427 26.26859691991686 25.93811637465853 24.386943531214182 92-93 23.398827432128112 26.27575420150822 25.928074711720683 24.397343654642984 94-95 23.3134241656576 26.225379749489814 26.043658587295315 24.41753749755727 96-97 23.471554284773656 26.123110750962063 26.009445213590173 24.395889750674108 98-99 23.42812673350539 26.35773551305291 25.763177317569152 24.450960435872542 100-101 23.31133443829659 26.528106861589855 25.924235743040953 24.236322957072602 102-103 23.071601012777485 26.529448343173833 25.905227866012183 24.493722778036503 104-105 22.98607516196353 26.69294185371437 25.79448390579741 24.526499078524687 106-107 23.087333205444498 26.680013205266047 25.818748801042148 24.41390478824731 108-109 23.032451451751992 26.859452883361524 25.80142696984608 24.306668695040404 110-111 23.076828697811763 27.10177862240028 25.6228613800625 24.19853129972546 112-113 23.094209917429854 27.242437316155723 25.5577065246343 24.10564624178013 114-115 23.090961900886622 27.3862139675333 25.472825737782156 24.04999839379792 116-117 23.062763638245716 27.299648318738694 25.600383538711263 24.037204504304324 118-119 23.244179797808997 27.383832973698752 25.368459069361034 24.00352815913122 120-121 23.19108207932231 27.576806107145412 25.362852124890328 23.869259688641947 122-123 23.25918080356346 27.633134589919944 25.251604342968907 23.856080263547685 124-125 23.392393699483954 27.791479628044403 25.020619704383602 23.795506968088038 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2900.0 1 2114.0 2 1168.5 3 866.0 4 664.5 5 558.0 6 498.5 7 501.0 8 548.5 9 576.0 10 587.5 11 612.0 12 622.5 13 714.0 14 835.0 15 905.5 16 1065.0 17 1278.0 18 1471.5 19 1697.0 20 2053.0 21 2593.5 22 3222.0 23 3951.0 24 4769.0 25 5770.5 26 7305.0 27 9223.0 28 10802.0 29 12705.0 30 15087.5 31 17871.0 32 21341.5 33 23766.5 34 25648.0 35 28946.0 36 34009.5 37 38772.0 38 42939.5 39 47166.5 40 51818.0 41 57002.0 42 60643.0 43 65863.0 44 71870.0 45 77906.0 46 84679.0 47 88609.0 48 91491.0 49 94740.5 50 99104.5 51 100653.0 52 97700.0 53 96256.0 54 94898.5 55 91554.0 56 86065.0 57 78930.0 58 73093.5 59 65700.0 60 55238.0 61 45052.5 62 35994.0 63 27053.0 64 19326.5 65 14210.0 66 10680.5 67 8001.0 68 5732.5 69 4020.0 70 3084.0 71 2346.5 72 1725.0 73 1411.0 74 1122.0 75 782.5 76 557.0 77 371.0 78 233.5 79 167.0 80 113.0 81 72.0 82 57.5 83 44.0 84 30.5 85 30.5 86 21.5 87 13.0 88 14.0 89 12.0 90 10.0 91 9.0 92 7.5 93 4.0 94 3.5 95 8.0 96 8.5 97 6.0 98 10.0 99 11.5 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16737520983206747 2 0.15038800819487574 3 0.17647071937009137 4 0.14918419075601966 5 0.1750885586069603 6 0.11133082017865542 7 0.1107957902058305 8 0.09849010083085696 9 0.10633720709895603 10-11 0.09120031745111722 12-13 0.07958570845770922 14-15 0.07869399183633434 16-17 0.07934048638683112 18-19 0.07635323570522524 20-21 0.07073542099056342 22-23 0.06277685014479249 24-25 0.06696791826525449 26-27 0.07697743734018765 28-29 0.07200611717602264 30-31 0.0687290585924699 32-33 0.07771310355282195 34-35 0.07584049864793467 36-37 0.07773539646835632 38-39 0.07309847003720688 40-41 0.07851564851205935 42-43 0.07844876976545623 44-45 0.07113669347018212 46-47 0.06777046322449189 48-49 0.06032462943601152 50-51 0.06616537330601709 52-53 0.06855071526819492 54-55 0.07015580518666972 56-57 0.07427999456052861 58-59 0.05990106404085845 60-61 0.07055707766628842 62-63 0.06554117167105465 64-65 0.05927686240589604 66-67 0.06395837466811422 68-69 0.06674498910991075 70-71 0.062397870580708156 72-73 0.056467955048565124 74-75 0.06041380109814902 76-77 0.06962077521384478 78-79 0.06340105177975491 80-81 0.06177366894574573 82-83 0.06661123161670453 84-85 0.0683277861128512 86-87 0.061573032705936386 88-89 0.06083736649330209 90-91 0.06384691009044235 92-93 0.05747113624761187 94-95 0.06772587739342315 96-97 0.05352529019802797 98-99 0.05352529019802797 100-101 0.051786442786346934 102-103 0.04944568665523784 104-105 0.048130404638709864 106-107 0.05952208447677412 108-109 0.059388326983567885 110-111 0.06667811036330765 112-113 0.06678957494097951 114-115 0.06924179564976046 116-117 0.07271949047312255 118-119 0.06551887875552029 120-121 0.06645518120796393 122-123 0.06460486921861101 124-125 0.07506024660423165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2242865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.49220957016411 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34804664655152 31.093036617562742 2 14.091472811358589 12.82104468573189 3 6.204983299438189 8.468352019122312 4 3.381090796630163 6.152531643842099 5 2.037066870730541 4.633533649585607 6 1.3058227900067985 3.56428584146914 7 0.9141465133574411 2.9110581334444072 8 0.6564044072165184 2.3889029484698554 9 0.48837054742552005 1.9995349762239774 >10 2.426730511530706 19.09664446242068 >50 0.1117030012480079 3.4187934804073747 >100 0.032090367281792535 2.4982270067177272 >500 0.0014795980172832496 0.4480614520481667 >1k 5.918392069132565E-4 0.505993082953915 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3262 0.1454389809462451 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2313 0.1031270272620064 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.4585831068744666E-5 7 0.0 0.0 0.0 4.4585831068744666E-5 4.4585831068744666E-5 8 0.0 0.0 0.0 4.4585831068744666E-5 4.4585831068744666E-5 9 0.0 0.0 0.0 4.4585831068744666E-5 4.4585831068744666E-5 10-11 0.0 0.0 0.0 6.6878746603117E-5 4.4585831068744666E-5 12-13 0.0 0.0 0.0 8.917166213748933E-5 1.33757493206234E-4 14-15 0.0 0.0 0.0 8.917166213748933E-5 2.229291553437233E-4 16-17 0.0 0.0 0.0 8.917166213748933E-5 3.5668664854995733E-4 18-19 0.0 0.0 0.0 1.5605040874060633E-4 3.7897956408432964E-4 20-21 0.0 0.0 0.0 2.00636239809351E-4 5.35029972824936E-4 22-23 0.0 0.0 0.0 3.3439373301558497E-4 5.35029972824936E-4 24-25 0.0 0.0 0.0 7.802520437030316E-4 5.35029972824936E-4 26-27 0.0 0.0 0.0 0.0015159182563373186 5.796158038936807E-4 28-29 0.0 0.0 0.0 0.004748391008821307 6.242016349624253E-4 30-31 0.0 0.0 0.0 0.015917141691541847 6.242016349624253E-4 32-33 0.0 0.0 0.0 0.04329284196775107 6.242016349624253E-4 34-35 0.0 0.0 0.0 0.08930541963069556 6.687874660311699E-4 36-37 0.0 0.0 0.0 0.16554719075824892 6.687874660311699E-4 38-39 0.0 0.0 0.0 0.28650855044775325 6.687874660311699E-4 40-41 0.0 0.0 0.0 0.42296348643364623 6.687874660311699E-4 42-43 0.0 0.0 0.0 0.5542687589310993 7.133732970999147E-4 44-45 0.0 0.0 0.0 0.7141535491436176 7.133732970999147E-4 46-47 0.0 0.0 0.0 0.9086592371810163 8.02544959237404E-4 48-49 2.2292915534372333E-5 0.0 0.0 1.1158495941574729 8.917166213748932E-4 50-51 8.917166213748933E-5 0.0 0.0 1.3401163244332583 8.917166213748932E-4 52-53 8.917166213748933E-5 0.0 0.0 1.5534595260972015 9.36302452443638E-4 54-55 8.917166213748933E-5 0.0 0.0 1.7982357386646097 9.36302452443638E-4 56-57 8.917166213748933E-5 0.0 0.0 2.0962697264436336 9.36302452443638E-4 58-59 8.917166213748933E-5 0.0 0.0 2.422905524853257 9.36302452443638E-4 60-61 8.917166213748933E-5 0.0 0.0 2.7714775521487027 9.808882835123827E-4 62-63 8.917166213748933E-5 0.0 0.0 3.1177088233130394 0.0010254741145811273 64-65 8.917166213748933E-5 0.0 0.0 3.487860392845757 0.0010477670301154997 66-67 8.917166213748933E-5 0.0 0.0 3.935100864296335 0.0010923528611842441 68-69 8.917166213748933E-5 0.0 0.0 4.427462196788483 0.0011146457767186166 70-71 8.917166213748933E-5 2.2292915534372333E-5 0.0 4.968845650540715 0.0011146457767186166 72-73 8.917166213748933E-5 4.4585831068744666E-5 0.0 5.505257784128782 0.0011592316077873614 74-75 8.917166213748933E-5 4.4585831068744666E-5 0.0 6.048959701096589 0.001203817438856106 76-77 8.917166213748933E-5 4.4585831068744666E-5 0.0 6.649865239325594 0.001203817438856106 78-79 8.917166213748933E-5 4.4585831068744666E-5 0.0 7.326009367483108 0.0012261103543904782 80-81 8.917166213748933E-5 4.4585831068744666E-5 0.0 8.049860334884176 0.0012484032699248506 82-83 8.917166213748933E-5 4.4585831068744666E-5 0.0 8.8063035447965 0.0012929891009935952 84-85 8.917166213748933E-5 4.4585831068744666E-5 0.0 9.537132194759828 0.0012929891009935952 86-87 8.917166213748933E-5 4.4585831068744666E-5 0.0 10.305479821567504 0.0013152820165279674 88-89 8.917166213748933E-5 4.4585831068744666E-5 0.0 11.15611059961255 0.0013375749320623399 90-91 8.917166213748933E-5 4.4585831068744666E-5 0.0 12.063075575212952 0.0013375749320623399 92-93 8.917166213748933E-5 4.4585831068744666E-5 0.0 12.980183827381495 0.001471332425268574 94-95 8.917166213748933E-5 4.4585831068744666E-5 0.0 13.900680602711265 0.0014936253408029464 96-97 8.917166213748933E-5 4.4585831068744666E-5 0.0 14.825167809921684 0.0016273828340091802 98-99 8.917166213748933E-5 4.4585831068744666E-5 0.0 15.829708876816037 0.0017388474116810419 100-101 8.917166213748933E-5 4.4585831068744666E-5 0.0 16.90915413990588 0.0017388474116810419 102-103 8.917166213748933E-5 4.4585831068744666E-5 0.0 18.00206432397848 0.0017388474116810419 104-105 8.917166213748933E-5 4.4585831068744666E-5 0.0 19.068824918129266 0.0017388474116810419 106-107 8.917166213748933E-5 4.4585831068744666E-5 0.0 20.132798897838256 0.0017388474116810419 108-109 8.917166213748933E-5 4.4585831068744666E-5 0.0 21.22860716093033 0.0017834332427497865 110-111 8.917166213748933E-5 4.4585831068744666E-5 0.0 22.388886535747805 0.0017834332427497865 112-113 8.917166213748933E-5 4.4585831068744666E-5 0.0 23.583051142177528 0.0017834332427497865 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11620 0.0 28.510517 1 CATGGGG 6980 0.0 27.90383 4 GTATCAA 7655 0.0 27.087597 1 TACATGG 11850 0.0 26.991528 2 ACATGGG 12035 0.0 25.68578 3 GGTATCA 5965 0.0 24.872837 1 AACGCAG 8385 0.0 23.710117 6 CAACGCA 8580 0.0 23.394518 5 TCAACGC 8565 0.0 23.365948 4 TATCAAC 9090 0.0 22.475105 2 ATCAACG 9035 0.0 22.216377 3 ATGGGGA 3320 0.0 21.887356 5 ACGCAGA 9480 0.0 20.593796 7 GTATAGG 465 0.0 20.502342 1 CGCAGAG 9740 0.0 19.981617 8 TAGTACA 455 5.456968E-12 19.635925 4 ATGGGGG 3065 0.0 18.85006 5 GCAGAGT 10560 0.0 18.035486 9 GAGTACT 2830 0.0 17.345942 12-13 CTATAGT 640 0.0 16.751898 4 >>END_MODULE