FastQCFastQC Report
Mon 15 Aug 2016
SRR2920944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242865
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.3199479237493117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.24990358314031383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.17455352863413534No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22550.10054104906001922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97300.038.7583731
GGTATCA68800.037.3809281
TATCGTG902.3814244E-433.0845535
TCAACGC121850.030.692474
CAACGCA123000.030.4055085
TATCAAC125500.029.9421772
AACGCAG126350.029.7712696
ATCAACG127300.029.472023
CATGGGG65450.027.8425854
GTATCGC1556.8182E-626.879546
GTACATG115150.026.2209171
ACGCAGA142300.026.176917
CCGTACG1150.001005333725.8685869
CGCAGAG146950.025.222048
TACATGG117950.024.8407612
GAGTACT81500.024.82225212-13
ACATGGG119950.024.1783393
AGTACTT84700.024.1303312-13
GTACTTT88100.023.03023514-15
GCAGAGT157400.023.020419
Produced by FastQC (version 0.11.5)