##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2242865
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.105666636199683	31.0	31.0	34.0	28.0	34.0
2	31.21216658158204	33.0	31.0	34.0	28.0	34.0
3	31.282953722136643	33.0	31.0	34.0	27.0	34.0
4	34.829356202892285	37.0	35.0	37.0	32.0	37.0
5	34.5299596721158	37.0	35.0	37.0	31.0	37.0
6	34.25220956232319	37.0	35.0	37.0	30.0	37.0
7	34.15146921459829	37.0	35.0	37.0	30.0	37.0
8	34.14840170942076	37.0	35.0	37.0	30.0	37.0
9	35.68504435175546	39.0	35.0	39.0	30.0	39.0
10-11	35.653086788549466	39.0	35.0	39.0	30.0	39.0
12-13	35.548084481232706	39.0	35.0	39.0	30.0	39.0
14-15	36.75883167288268	40.0	37.0	41.0	30.0	41.0
16-17	36.76626390799268	40.0	37.0	41.0	30.0	41.0
18-19	36.80663236530063	40.0	37.0	41.0	30.0	41.0
20-21	36.80537170092717	40.0	37.0	41.0	30.0	41.0
22-23	36.74869530711835	40.0	37.0	41.0	30.0	41.0
24-25	36.72254705477147	40.0	37.0	41.0	30.0	41.0
26-27	36.58604998517521	40.0	36.5	41.0	30.0	41.0
28-29	36.47673243819847	40.0	36.0	41.0	29.5	41.0
30-31	36.313069890519486	40.0	36.0	41.0	29.0	41.0
32-33	36.137674581394776	40.0	35.5	41.0	28.5	41.0
34-35	35.99993601933242	40.0	35.0	41.0	27.5	41.0
36-37	35.85166895912148	40.0	35.0	41.0	27.0	41.0
38-39	35.71493157189577	40.0	35.0	41.0	27.0	41.0
40-41	35.55445891750061	40.0	35.0	41.0	26.0	41.0
42-43	35.38892086683773	40.0	35.0	41.0	25.5	41.0
44-45	35.168283200281785	39.0	35.0	41.0	24.5	41.0
46-47	34.911788493734576	39.0	35.0	41.0	23.5	41.0
48-49	34.61277361767204	39.0	34.0	41.0	22.5	41.0
50-51	34.39839758523139	39.0	34.0	41.0	21.5	41.0
52-53	34.183054486114855	39.0	34.0	41.0	20.0	41.0
54-55	33.9516219210697	38.0	34.0	41.0	19.0	41.0
56-57	33.684168017245796	38.0	33.0	40.0	18.0	41.0
58-59	33.43316048892822	37.5	33.0	40.0	17.0	41.0
60-61	33.14369522909315	37.0	33.0	40.0	13.5	41.0
62-63	32.837420219228534	36.5	32.5	40.0	13.0	41.0
64-65	32.510722446513725	36.0	32.0	39.0	9.5	41.0
66-67	32.17395786193106	35.5	32.0	39.0	8.0	41.0
68-69	31.79949105273835	35.0	32.0	38.5	7.0	40.5
70-71	31.459188805389537	35.0	31.0	37.5	4.0	40.0
72-73	31.10227075637633	35.0	31.0	37.0	2.0	39.5
74-75	30.785778680393157	35.0	31.0	36.5	2.0	39.0
76-77	30.5851839054067	35.0	31.0	36.0	2.0	39.0
78-79	30.447019325728476	35.0	31.0	36.0	2.0	37.5
80-81	30.217536276146802	35.0	31.0	35.0	2.0	37.0
82-83	30.017826975765374	35.0	31.0	35.0	2.0	37.0
84-85	29.794464669072816	35.0	31.0	35.0	2.0	36.0
86-87	29.64577538104166	35.0	31.0	35.0	2.0	36.0
88-89	29.511140215750835	35.0	30.5	35.0	2.0	36.0
90-91	29.368951987747813	35.0	30.0	35.0	2.0	35.0
92-93	29.252187715265965	34.5	30.0	35.0	2.0	35.0
94-95	29.16949682660347	34.0	30.0	35.0	2.0	35.0
96-97	29.10795433519182	34.0	30.0	35.0	2.0	35.0
98-99	29.008424715709594	34.0	30.0	35.0	2.0	35.0
100-101	28.906409213216133	34.0	30.0	35.0	2.0	35.0
102-103	27.509059617944015	32.5	26.5	34.0	2.0	35.0
104-105	28.570727618470126	34.0	29.0	35.0	2.0	35.0
106-107	28.75072975859002	34.0	29.0	35.0	2.0	35.0
108-109	28.84478981124588	34.5	29.5	35.0	2.0	35.0
110-111	29.017190067168556	35.0	31.0	35.0	2.0	35.0
112-113	28.986615779371476	35.0	31.0	35.0	2.0	35.0
114-115	28.899021118078885	35.0	31.0	35.0	2.0	35.0
116-117	28.78524900072006	35.0	30.0	35.0	2.0	35.0
118-119	28.684015979561856	35.0	30.0	35.0	2.0	35.0
120-121	28.500284011743908	35.0	30.0	35.0	2.0	35.0
122-123	28.30495437754836	35.0	30.0	35.0	2.0	35.0
124-125	27.584340564412035	34.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43457.0
3	17386.0
4	7532.0
5	6302.0
6	7578.0
7	8844.0
8	9734.0
9	10611.0
10	12160.0
11	13874.0
12	15042.0
13	17381.0
14	21059.0
15	19428.0
16	11981.0
17	10354.0
18	9840.0
19	9106.0
20	9038.0
21	9593.0
22	10007.0
23	11110.0
24	14537.0
25	19694.0
26	24424.0
27	28796.0
28	34422.0
29	40904.0
30	48864.0
31	59369.0
32	73975.0
33	94493.0
34	127212.0
35	192403.0
36	368284.0
37	613233.0
38	210338.0
39	500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15895461331301	18.074780144480634	12.302123329998432	25.46414191220793
2	16.79118237080576	20.46311369967035	36.982033621246636	25.763670308277252
3	19.348980672821924	23.899035692056366	29.54443717195785	27.20754646316386
4	13.480276973974611	15.90125779713841	35.076042999629934	35.54242222925705
5	13.890610588205371	37.293435127039096	33.963185234468675	14.852769050286858
6	33.65252113754875	35.45241915804904	16.157906734706533	14.737152969695671
7	28.678680151882613	32.13006710200164	20.588487141087246	18.6027656050285
8	27.25479283945861	34.01167975912727	19.509951340282683	19.223576061131446
9	27.036169485071	14.432745127154828	18.97634912629689	39.55473626147729
10-11	25.90782960534266	25.186867734252967	27.136789009684612	21.768513650719758
12-13	26.36670709629088	22.760102203711796	27.47685701290478	23.396333687092547
14-15	23.80909060098002	23.96599809846932	25.079914234901473	27.144997065649186
16-17	23.30258888419145	26.854701037658607	25.977810838243347	23.8648992399066
18-19	23.339665171449067	25.509822970903794	28.265476973819904	22.885034883827235
20-21	24.22112038442535	24.682499478264592	28.02466069361378	23.071719443696274
22-23	23.85612892825777	24.63261123110103	27.892204832044953	23.61905500859624
24-25	23.521349997536355	24.894503573177968	28.0094211592155	23.574725270070186
26-27	23.29641958160451	24.982799098374	28.130909674643217	23.589871645378274
28-29	23.473968424549568	24.93684686954447	28.236349893983004	23.352834811922953
30-31	23.639056064402293	24.9942367365794	27.875288776284158	23.491418422734146
32-33	23.339067835684656	25.152013363263332	27.81427614616471	23.694642654887303
34-35	23.534971454944966	25.086114539193293	28.04075348688912	23.33816051897262
36-37	23.877157956652013	24.815548302598444	27.740152876955946	23.567140863793597
38-39	23.702891244212093	25.062823566452057	27.671311559848395	23.562973629487452
40-41	23.633025027203484	25.00626572004495	27.81863750691236	23.542071745839205
42-43	23.711486864188622	24.897091309068117	27.715521825829057	23.6759000009142
44-45	23.617332013342608	25.060625925331337	27.555564474411803	23.766477586914252
46-47	23.828152610235808	25.138538234055307	27.179693114371613	23.853616041337272
48-49	23.74888098346364	25.402115735574306	26.873988983191623	23.97501429777044
50-51	23.613733989787534	25.598792982819134	26.816824396123522	23.97064863126981
52-53	23.779838784835235	25.461998677423857	26.64808103833375	24.11008149940716
54-55	23.54414337359982	25.622603933061523	26.559915664686862	24.2733370286518
56-57	23.536339765750146	25.667858251367292	26.476102305038545	24.319699677844017
58-59	23.359803076703543	25.395970341104057	26.78324056102627	24.460986021166136
60-61	23.47756985548402	25.507335500307477	26.79139923235781	24.22369541185069
62-63	23.255810842058953	25.463191626616567	26.913870183479382	24.3671273478451
64-65	23.1686643535907	25.48916517667918	26.85935164752593	24.48281882220419
66-67	23.124135415263783	25.647853243346656	26.84076835327768	24.387242988111876
68-69	23.37425736746325	25.338512902174287	26.772496587558063	24.514733142804396
70-71	23.133955476378667	25.21730599649724	26.927705134965503	24.72103339215859
72-73	23.247818804217722	25.298994625276716	26.76863716069049	24.68454940981507
74-75	23.15690925200809	25.457347458318676	26.686550272435277	24.69919301723796
76-77	23.118988116193357	25.284208277975083	26.820913947916015	24.77588965791555
78-79	23.11516355264173	25.43510804289341	26.658324745405665	24.791403659059196
80-81	23.0886888677764	25.4171320171301	26.649157820641427	24.84502129445207
82-83	22.910117803180377	25.317260595029023	26.761100358446114	25.011521243344486
84-85	22.864336957840138	25.395471685324317	26.512946196051573	25.22724516078398
86-87	22.88174744492412	25.269309167414715	26.595286415098883	25.253656972562283
88-89	22.713470069942453	25.249940189160558	26.59971420117802	25.43687553971897
90-91	22.6796115639001	25.302012300550814	26.592845525192494	25.42553061035659
92-93	22.630630317283437	25.271819952920065	26.566763386098714	25.530786343697788
94-95	22.482642159714203	25.18310985878061	26.73202161176808	25.602226369737103
96-97	22.684352588162422	24.95956588453167	26.702212717114588	25.653868810191316
98-99	22.587728734821336	25.139967104859778	26.443353984394317	25.828950175924565
100-101	22.45419841456179	25.153586121384375	26.632802245201333	25.7594132188525
102-103	22.30175289315114	24.946667995646475	26.62437121527845	26.12720789592393
104-105	22.152249661067696	25.136147004654426	26.53947499547979	26.17212833879809
106-107	22.09479593934251	25.130635473188235	26.586007613175422	26.18856097429383
108-109	22.0459407485856	25.226208857055266	26.623271369248346	26.10457902511078
110-111	22.015608717230457	25.353463602178373	26.498321362815275	26.132606317775895
112-113	21.92182745213624	25.49506909597455	26.405602500444232	26.177500951444983
114-115	21.843944498359907	25.53945374532624	26.353870777232085	26.262730979081766
116-117	21.61068319381124	25.485927032359466	26.587419602947243	26.315970170882053
118-119	21.717796608280217	25.480768587690022	26.406770063513935	26.39466474051583
120-121	21.610955056179776	25.696651507044766	26.401752273943284	26.290641162832173
122-123	21.509158534812777	25.741876543253913	26.281419066837998	26.467545855095313
124-125	21.358044369738156	25.792632835568018	26.335311093103332	26.514011701590494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	117.0
2	307.5
3	621.0
4	468.0
5	242.5
6	510.0
7	1132.0
8	1876.5
9	2066.0
10	1531.0
11	1083.0
12	1065.5
13	1173.5
14	1301.0
15	1437.5
16	1606.0
17	1787.5
18	1960.5
19	2178.0
20	2487.5
21	2872.5
22	3364.0
23	4004.5
24	4781.5
25	5637.0
26	6945.0
27	8835.5
28	10538.0
29	12515.0
30	14928.5
31	17997.0
32	21816.0
33	24141.5
34	26079.0
35	29192.5
36	33803.0
37	38549.5
38	42582.0
39	46720.0
40	51284.5
41	56363.0
42	60226.0
43	65081.0
44	71318.0
45	77679.0
46	84137.0
47	88155.0
48	90698.5
49	93967.5
50	99216.0
51	101369.0
52	97986.5
53	96790.5
54	95912.5
55	92475.0
56	87102.5
57	79584.0
58	73575.5
59	66296.5
60	55715.5
61	45305.0
62	35847.0
63	26752.0
64	19164.0
65	13909.5
66	10096.5
67	7472.5
68	5256.5
69	3591.0
70	2742.5
71	2040.5
72	1520.0
73	1194.0
74	879.5
75	623.0
76	432.0
77	271.5
78	158.5
79	94.5
80	59.5
81	41.0
82	34.5
83	23.0
84	12.0
85	6.0
86	7.5
87	7.5
88	7.0
89	7.0
90	4.5
91	5.5
92	6.0
93	4.0
94	9.0
95	15.5
96	11.0
97	4.0
98	3.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21927311719608625
2	0.007847106268099062
3	5.796158038936806E-4
4	0.0024522207087809564
5	0.08301881745000257
6	0.05519725886310589
7	0.027063599458728015
8	0.015738798367266868
9	0.012662376023523485
10-11	0.010522256132223741
12-13	0.01270696185459223
14-15	0.02055406812269129
16-17	0.022694188013991035
18-19	0.02200310763242549
20-21	0.015560455042991887
22-23	0.014066829702188942
24-25	0.011302508175926772
26-27	0.011146457767186166
28-29	0.013375749320623399
30-31	0.009452196186573869
32-33	0.013331163489554654
34-35	0.015939434607076218
36-37	0.018191019076047824
38-39	0.02113368392658497
40-41	0.022515844689716055
42-43	0.020710118531431898
44-45	0.018948978204216482
46-47	0.01932795776830081
48-49	0.017054080383794832
50-51	0.013910779293448333
52-53	0.012127346050698548
54-55	0.01729930245467293
56-57	0.021089098095516227
58-59	0.016385292917763663
60-61	0.01845853406246029
62-63	0.02539163079365009
64-65	0.01767828201875726
66-67	0.0204648964605538
68-69	0.014980839239098206
70-71	0.015493576296388771
72-73	0.023050874662540993
74-75	0.013999950955585826
76-77	0.012283396459439154
78-79	0.016764272481847996
80-81	0.016920322890588597
82-83	0.011971295641957942
84-85	0.015738798367266868
86-87	0.016407585833298037
88-89	0.016875737059519856
90-91	0.01259549727692037
92-93	0.013197405996348421
94-95	0.012952183925470326
96-97	0.014624152590548249
98-99	0.012684668939057857
100-101	0.017544524525551025
102-103	0.024232399185862726
104-105	0.007156025886533518
106-107	0.007757934605961572
108-109	0.01348721389829526
110-111	0.012127346050698548
112-113	0.009964933243864433
114-115	0.00697768256225854
116-117	0.00401272479618702
118-119	0.002229291553437233
120-121	0.0028980790194684035
122-123	0.0017388474116810416
124-125	3.3439373301558497E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2242865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.01660667665551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.66586459104147	29.784217471397035
2	14.236311543885819	12.53268251507105
3	6.386114590672319	8.432852823890231
4	3.4421631367782806	6.0604936363380935
5	2.103172552449285	4.628725950714891
6	1.3713701430564698	3.621783611701526
7	0.9438425237494198	2.908132159280607
8	0.6734311233601129	2.3713722304608296
9	0.5128368653824594	2.0316004733545783
>10	2.502251032032007	19.277407407077767
>50	0.12271565844656511	3.6488979129358654
>100	0.036677797549012486	2.819252599989279
>500	0.0021317897978951703	0.6006279453880948
>1k	9.136241990978578E-4	0.7108607337582648
>5k	2.0302759979952395E-4	0.5710925286419248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7176	0.3199479237493117	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5605	0.24990358314031383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3915	0.17455352863413534	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2255	0.10054104906001922	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.229291553437233E-4
2	0.0	0.0	0.0	0.0	2.229291553437233E-4
3	0.0	0.0	0.0	0.0	2.229291553437233E-4
4	0.0	0.0	0.0	0.0	2.229291553437233E-4
5	0.0	0.0	0.0	0.0	2.67514986412468E-4
6	0.0	0.0	0.0	0.0	2.67514986412468E-4
7	0.0	0.0	0.0	0.0	2.67514986412468E-4
8	0.0	0.0	0.0	0.0	2.67514986412468E-4
9	0.0	0.0	0.0	0.0	2.67514986412468E-4
10-11	0.0	0.0	0.0	2.2292915534372333E-5	2.67514986412468E-4
12-13	0.0	0.0	0.0	4.4585831068744666E-5	6.464945504967976E-4
14-15	0.0	0.0	0.0	4.4585831068744666E-5	7.802520437030316E-4
16-17	0.0	0.0	0.0	4.4585831068744666E-5	8.917166213748932E-4
18-19	0.0	0.0	0.0	6.6878746603117E-5	0.001003181199046755
20-21	0.0	0.0	0.0	1.1146457767186166E-4	0.0010254741145811273
22-23	0.0	0.0	0.0	2.452220708780957E-4	0.001070059945649872
24-25	0.0	0.0	0.0	6.910803815655423E-4	0.001070059945649872
26-27	0.0	0.0	0.0	0.0013821607631310847	0.0011146457767186166
28-29	0.0	0.0	0.0	0.004503168937943211	0.001136938692252989
30-31	0.0	0.0	0.0	0.01558274795852626	0.0011592316077873612
32-33	0.0	0.0	0.0	0.04249029700851366	0.0011592316077873612
34-35	0.0	0.0	0.0	0.08816848093844257	0.001203817438856106
36-37	0.0	0.0	0.0	0.1646331812213397	0.001203817438856106
38-39	0.0	0.0	0.0	0.2844353093030566	0.001203817438856106
40-41	0.0	0.0	0.0	0.4193520341170779	0.001203817438856106
42-43	0.0	0.0	0.0	0.5492751458513998	0.001203817438856106
44-45	0.0	0.0	0.0	0.7079115327939933	0.001203817438856106
46-47	0.0	0.0	0.0	0.900500030095436	0.0012261103543904782
48-49	0.0	0.0	0.0	1.1050152372077677	0.001270696185459223
50-51	0.0	0.0	0.0	1.326985797183513	0.0012929891009935952
52-53	0.0	0.0	0.0	1.5380774143784848	0.0012929891009935952
54-55	0.0	0.0	0.0	1.7808918503788682	0.0012929891009935952
56-57	0.0	0.0	0.0	2.0760946378850265	0.0013152820165279674
58-59	0.0	0.0	0.0	2.3999661147683877	0.0013375749320623399
60-61	0.0	0.0	0.0	2.7471336883851682	0.0013375749320623399
62-63	0.0	0.0	0.0	3.091670697968892	0.0014267465941998293
64-65	0.0	0.0	0.0	3.4597267334413795	0.0014267465941998293
66-67	0.0	0.0	0.0	3.9029544800957705	0.0014267465941998293
68-69	0.0	0.0	0.0	4.3908572294810435	0.0014267465941998293
70-71	0.0	0.0	0.0	4.926511403940941	0.0014490395097342015
72-73	0.0	0.0	0.0	5.457907631533775	0.0015159182563373186
74-75	0.0	0.0	0.0	5.995991733786919	0.0015605040874060632
76-77	0.0	0.0	0.0	6.5929960117974105	0.0015605040874060632
78-79	0.0	0.0	0.0	7.264547799354843	0.0015605040874060632
80-81	0.0	0.0	0.0	7.982847830787854	0.0015605040874060632
82-83	0.0	0.0	0.0	8.730975783205857	0.0016942615806122972
84-85	0.0	0.0	0.0	9.456788527173948	0.0016942615806122972
86-87	0.0	0.0	0.0	10.219763561337842	0.0017388474116810419
88-89	0.0	0.0	0.0	11.061856152733224	0.0017834332427497865
90-91	0.0	0.0	0.0	11.961130072474269	0.0017834332427497865
92-93	0.0	0.0	0.0	12.871483571235897	0.0019617765670247654
94-95	0.0	0.0	0.0	13.783821139480086	0.00200636239809351
96-97	0.0	0.0	0.0	14.704585429796264	0.0022069986379028607
98-99	0.0	0.0	0.0	15.703999126117711	0.0023184632155747223
100-101	0.0	0.0	0.0	16.78068006768129	0.0023184632155747223
102-103	0.0	0.0	0.0	17.870580708156755	0.0023407561311090948
104-105	0.0	0.0	0.0	18.936204363615285	0.002363049046643467
106-107	0.0	0.0	0.0	20.001359867847597	0.002363049046643467
108-109	0.0	0.0	0.0	21.10008850287467	0.002363049046643467
110-111	0.0	0.0	0.0	22.263622643360165	0.002407634877712212
112-113	0.0	0.0	0.0	23.462513347883178	0.0024522207087809564
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9730	0.0	38.758373	1
GGTATCA	6880	0.0	37.380928	1
TATCGTG	90	2.3814244E-4	33.084553	5
TCAACGC	12185	0.0	30.69247	4
CAACGCA	12300	0.0	30.405508	5
TATCAAC	12550	0.0	29.942177	2
AACGCAG	12635	0.0	29.771269	6
ATCAACG	12730	0.0	29.47202	3
CATGGGG	6545	0.0	27.842585	4
GTATCGC	155	6.8182E-6	26.87954	6
GTACATG	11515	0.0	26.220917	1
ACGCAGA	14230	0.0	26.17691	7
CCGTACG	115	0.0010053337	25.868586	9
CGCAGAG	14695	0.0	25.22204	8
TACATGG	11795	0.0	24.840761	2
GAGTACT	8150	0.0	24.822252	12-13
ACATGGG	11995	0.0	24.178339	3
AGTACTT	8470	0.0	24.13033	12-13
GTACTTT	8810	0.0	23.030235	14-15
GCAGAGT	15740	0.0	23.02041	9
>>END_MODULE