FastQCFastQC Report
Mon 15 Aug 2016
SRR2920943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364061
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA2052.4228939E-929.0315067
GTATCAA55600.027.4390051
GTACATG81900.024.8854451
TCAACGC60150.024.0606234
TACATGG82050.023.8099822
ATCAACG62750.023.4448283
CATGGGG44300.023.3927754
ACCGTAT2552.4781912E-823.3360888
CAACGCA61650.023.1853895
AACGCAG62200.023.062936
ACATGGG82950.022.3309823
TATCAAC68100.022.127752
GTATATA13500.022.0718861
GTGTACG3005.68798E-921.8511661
GTATAGG5350.021.1642551
GGTATCA42850.021.1395591
ACGCAGA69200.020.4688917
CGCAGAG70400.020.1174348
GTGTAGC19100.019.0326671
GTAGCCC17900.018.9664333