##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920943_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2364061
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.091940943994253	31.0	30.0	34.0	22.0	34.0
2	29.137615738341776	31.0	30.0	34.0	22.0	34.0
3	29.296948767396444	31.0	30.0	34.0	22.0	34.0
4	32.67195685728922	37.0	33.0	37.0	24.0	37.0
5	32.74349392845616	37.0	35.0	37.0	25.0	37.0
6	32.69935208947654	37.0	33.0	37.0	25.0	37.0
7	32.62545382712206	37.0	33.0	37.0	23.0	37.0
8	32.53857493524913	37.0	33.0	37.0	22.0	37.0
9	33.87265176321593	39.0	34.0	39.0	22.0	39.0
10-11	33.869303922360714	39.0	34.0	39.0	22.0	39.0
12-13	33.79309607493208	39.0	34.0	39.0	21.0	39.0
14-15	34.86246843884316	40.0	34.0	41.0	20.0	41.0
16-17	34.833591645900846	40.0	34.0	41.0	20.0	41.0
18-19	34.80483096671364	39.5	34.0	41.0	19.0	41.0
20-21	34.83103312477977	40.0	34.0	41.0	20.0	41.0
22-23	34.733267034987676	39.0	34.0	41.0	19.5	41.0
24-25	34.72793553127436	39.0	34.0	41.0	21.0	41.0
26-27	34.65153712192706	39.0	34.0	41.0	20.0	41.0
28-29	34.54235762105969	39.0	34.0	41.0	19.0	41.0
30-31	34.39450018421691	39.0	34.0	41.0	18.0	41.0
32-33	34.26814811462141	39.0	34.0	41.0	17.5	41.0
34-35	34.203891946950606	39.0	33.5	41.0	16.5	41.0
36-37	34.06366544687299	39.0	33.0	41.0	16.0	41.0
38-39	33.91852769450534	39.0	33.0	41.0	15.0	41.0
40-41	33.79805195382014	39.0	33.0	41.0	14.0	41.0
42-43	33.6080299958419	38.5	33.0	41.0	9.0	41.0
44-45	33.44291813959115	38.0	33.0	40.5	9.0	41.0
46-47	33.24701266168682	38.0	32.0	40.0	8.0	41.0
48-49	33.13749920158575	38.0	32.0	40.0	7.5	41.0
50-51	32.947638829962514	38.0	32.0	40.0	4.0	41.0
52-53	32.75706041426173	38.0	32.0	40.0	2.0	41.0
54-55	32.52321873251155	38.0	31.0	40.0	2.0	41.0
56-57	32.264794986254586	37.5	31.0	40.0	2.0	41.0
58-59	31.99949282188573	37.0	31.0	40.0	2.0	41.0
60-61	31.74262466154638	37.0	30.5	40.0	2.0	41.0
62-63	31.436776377597702	36.0	30.0	40.0	2.0	41.0
64-65	31.162033043986597	36.0	30.0	39.0	2.0	41.0
66-67	30.81853112081287	35.0	29.0	39.0	2.0	40.5
68-69	30.49445699582202	35.0	29.0	38.5	2.0	40.0
70-71	30.15926027289482	35.0	29.0	38.0	2.0	40.0
72-73	30.209011527198328	35.0	29.5	37.5	2.0	40.0
74-75	30.2038117882745	35.0	30.0	37.0	2.0	39.0
76-77	29.92694985450883	35.0	30.0	37.0	2.0	39.0
78-79	29.62966987738472	35.0	30.0	36.0	2.0	39.0
80-81	29.35455007294651	35.0	29.0	36.0	2.0	37.5
82-83	29.10452247213587	35.0	29.0	35.5	2.0	37.0
84-85	28.858936169582762	34.0	29.0	35.0	2.0	37.0
86-87	28.655401658417443	34.0	29.0	35.0	2.0	36.0
88-89	28.4542346834536	34.0	29.0	35.0	2.0	36.0
90-91	28.277759541737716	34.0	29.0	35.0	2.0	36.0
92-93	28.118711826809886	34.0	29.0	35.0	2.0	35.0
94-95	28.00613753198416	34.0	29.0	35.0	2.0	35.0
96-97	27.913544532057337	34.0	29.0	35.0	2.0	35.0
98-99	27.79143516178305	34.0	28.0	35.0	2.0	35.0
100-101	27.67166562114937	34.0	27.5	35.0	2.0	35.0
102-103	26.335920900518218	32.0	24.5	34.0	2.0	35.0
104-105	27.350843738803697	34.0	27.0	35.0	2.0	35.0
106-107	27.51806023617834	34.0	27.0	35.0	2.0	35.0
108-109	27.455869159044543	34.0	27.0	35.0	2.0	35.0
110-111	27.36752604945473	34.0	27.0	35.0	2.0	35.0
112-113	27.262188031527106	34.0	27.0	35.0	2.0	35.0
114-115	27.162743685547877	34.0	27.0	35.0	2.0	35.0
116-117	26.95585731501852	34.0	25.5	35.0	2.0	35.0
118-119	26.779731572070265	34.0	25.0	35.0	2.0	35.0
120-121	26.61426947105003	34.0	25.0	35.0	2.0	35.0
122-123	26.327731391025864	34.0	25.0	35.0	2.0	35.0
124-125	25.066664311961492	32.5	20.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	120556.0
3	17390.0
4	11265.0
5	10161.0
6	11964.0
7	12788.0
8	13066.0
9	13774.0
10	14626.0
11	15510.0
12	15629.0
13	15213.0
14	14834.0
15	13916.0
16	11822.0
17	11049.0
18	10615.0
19	10396.0
20	10584.0
21	11371.0
22	13270.0
23	17113.0
24	22996.0
25	28140.0
26	32188.0
27	36261.0
28	42032.0
29	48716.0
30	57106.0
31	68576.0
32	83312.0
33	105587.0
34	139582.0
35	200410.0
36	349442.0
37	558194.0
38	194356.0
39	251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.995689808592246	18.677213612950574	11.939128504043241	25.387968074413937
2	15.73247530666757	21.11470034378965	38.723339356183445	24.42948499335933
3	18.93566270645551	24.770677967753596	29.523566978305354	26.77009234748554
4	12.527981454713677	16.60149663714175	37.20542158160248	33.66510032654209
5	13.325434720336554	38.364249792788534	34.736354129059265	13.573961357815644
6	32.35846116320668	37.03706840479159	17.172424657162992	13.432045774838745
7	27.44446152419403	32.94416912170423	21.599037210281217	18.012332143820526
8	25.255741914067332	36.407698339709945	20.254420863120494	18.082138883102232
9	25.86462058321001	16.22926974891863	19.474378436516155	38.431731231355215
10-11	24.525931334268027	26.845188057769093	28.3420631642767	20.286817443686186
12-13	24.844683159148033	24.431718481280594	27.91624288927107	22.807355470300305
14-15	22.404578625756102	26.002399888070133	25.170588268911924	26.422433217261837
16-17	22.580206806688324	28.55752207893808	26.087712079302143	22.774559035071455
18-19	22.497080115533873	28.069984942328315	27.403861825013514	22.0290731171243
20-21	23.503396429278183	27.25365966709808	27.020465351553447	22.222478552070292
22-23	23.007112881277735	27.119568782895225	26.740730080863788	23.132588254963252
24-25	22.699076574555516	27.14003508136269	27.09920982278155	23.061678521300244
26-27	22.757956947574982	27.23822255768259	27.023153044786145	22.980667449956282
28-29	22.660741819382462	27.51889788527822	27.07756096373659	22.742799331602725
30-31	23.03877517762168	27.414840008211762	26.88418664403494	22.66219817013162
32-33	22.685742563862895	27.445086206020708	26.874781987327324	22.994389242789072
34-35	22.839813073944754	27.39153744542716	27.035722653682846	22.732926826945242
36-37	22.933877387911508	27.249357925006656	26.964673281510144	22.85209140557169
38-39	22.839522504032505	27.415380896817194	27.000796447459123	22.744300151691178
40-41	22.908476243734288	27.33548494429701	27.03805802999753	22.71798078197117
42-43	22.81391516747328	27.304944348431626	27.008213593650947	22.87292689044414
44-45	22.883116723694823	27.383102860847274	26.90175516348742	22.83202525197048
46-47	22.642821209228142	27.37003598532989	26.876383206620304	23.110759598821666
48-49	22.654413331660088	27.333069084997458	27.004945450636175	23.007572132706276
50-51	22.58583365131609	27.465435115751013	26.89056885368963	23.05816237924327
52-53	22.658689174088245	27.455682164505095	26.758004300565506	23.12762436084115
54-55	22.711328192627064	27.279549925056646	26.803878683621374	23.205243198694912
56-57	22.657257996241047	27.414153642967204	26.796593237271203	23.131995123520547
58-59	22.695083489530766	27.202457906052135	26.92152472963224	23.180933874784856
60-61	22.729138107540837	27.17148301310074	26.98370973705604	23.115669142302387
62-63	22.553143218014917	27.160405514255686	26.999878520112542	23.28657274761686
64-65	22.46017110310416	27.11431436227419	27.013686639982154	23.411827894639494
66-67	22.509821964460617	27.225486569241358	26.886701809307016	23.377989656991012
68-69	22.719282354046687	27.00416761551948	26.893501042538798	23.383048987895037
70-71	22.383206743375457	26.808070718250754	27.173301617064144	23.63542092130965
72-73	22.43717936834638	26.755932465942838	27.25920856437012	23.54767960134066
74-75	22.319756712829715	26.882639758913758	27.161818982155555	23.63578454610097
76-77	22.23018251192315	26.73407915015994	27.36093609056133	23.674802247355572
78-79	22.34541423499474	26.74980910634681	27.30213089802131	23.602645760637138
80-81	22.26359591342211	26.740758376585244	27.354041341946676	23.641604368045964
82-83	22.25579997544984	26.693051034704613	27.425640526389305	23.625508463456242
84-85	22.35889406496544	26.58211961498795	27.28881597090166	23.770170349144955
86-87	22.18632981985654	26.540696185628054	27.43366783528604	23.839306159229363
88-89	22.239267698678677	26.46505975957342	27.473764451032196	23.821908090715702
90-91	22.280919885167787	26.485670840427456	27.502060778958576	23.73134849544618
92-93	22.24848927973217	26.370155299186628	27.451673352174794	23.92968206890641
94-95	22.04819164912863	26.42472922567941	27.530895447334245	23.99618367785771
96-97	22.060343096374204	26.347153838942734	27.594759704089057	23.997743360594008
98-99	22.03155048890815	26.328499553075652	27.49228039762724	24.14766956038896
100-101	21.96271857200505	26.420864835514337	27.510537523189534	24.105879069291078
102-103	21.82733383045878	26.403222625584128	27.612913355435946	24.156530188521142
104-105	21.666746016379896	26.439853446369643	27.6002213328001	24.293179204450354
106-107	21.55585333201845	26.456883806937583	27.64315525858796	24.344107602456006
108-109	21.56036836693298	26.395369531914458	27.654078588529174	24.390183512623384
110-111	21.52521650168327	26.575014163958212	27.544237369527035	24.355531964831478
112-113	21.515810610868986	26.58166954954505	27.588775758340223	24.31374408124574
114-115	21.382590652696027	26.584120571704794	27.62478634721678	24.408502428382402
116-117	21.384250270965744	26.528461615144195	27.61268439269069	24.474603721199372
118-119	21.38138569036481	26.43912419980462	27.592264792080417	24.587225317750157
120-121	21.39616192506918	26.541764329289265	27.57913450193385	24.482939243707705
122-123	21.388907818553452	26.516340204025568	27.53833045437826	24.556421523042715
124-125	21.3679519243306	26.599368575052196	27.377008444398538	24.655671056218665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1818.0
1	1307.0
2	620.5
3	408.0
4	336.5
5	278.5
6	265.5
7	306.0
8	341.5
9	355.5
10	369.5
11	385.5
12	416.0
13	490.0
14	581.5
15	645.5
16	754.0
17	945.0
18	1168.0
19	1456.0
20	1878.5
21	2409.5
22	3060.5
23	4086.5
24	5398.0
25	6935.5
26	9398.0
27	12562.5
28	15394.0
29	18794.0
30	23057.0
31	27413.0
32	32234.0
33	37158.0
34	41871.0
35	46365.5
36	52052.0
37	57600.0
38	61392.0
39	65445.0
40	70508.0
41	75302.5
42	78994.0
43	83651.0
44	88300.0
45	91505.5
46	94289.5
47	96620.5
48	98903.5
49	100595.0
50	101751.5
51	99317.5
52	93936.5
53	89294.5
54	83942.0
55	77504.0
56	70202.0
57	62586.5
58	55391.0
59	48114.0
60	39886.5
61	31631.0
62	24412.5
63	18307.0
64	13649.0
65	10245.5
66	7499.5
67	5533.5
68	4137.5
69	2992.5
70	2197.5
71	1573.0
72	1138.0
73	917.5
74	670.5
75	484.0
76	338.5
77	208.0
78	151.5
79	110.0
80	72.5
81	52.5
82	44.5
83	37.0
84	31.0
85	29.0
86	23.0
87	20.5
88	17.0
89	10.5
90	10.0
91	9.0
92	5.5
93	4.5
94	8.0
95	9.5
96	7.5
97	5.5
98	5.5
99	6.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1722882785173479
2	0.15253413511749483
3	0.1763913875318784
4	0.15096903167896258
5	0.17567228595201226
6	0.10951494060432451
7	0.10955724069725782
8	0.09750171421126613
9	0.10473503010286114
10-11	0.09054334892373758
12-13	0.07924922411054537
14-15	0.07899542355294555
16-17	0.07846667239127925
18-19	0.07656316820928055
20-21	0.06994320366521845
22-23	0.06116593438155784
24-25	0.06569204432542138
26-27	0.07620361741934747
28-29	0.07163520738255062
30-31	0.06751094832155347
32-33	0.07669006848808047
34-35	0.0734541113786827
36-37	0.07605556709408091
38-39	0.07171980756841723
40-41	0.07614016727994752
42-43	0.07692271899921364
44-45	0.06901260162068576
46-47	0.0664322959517542
48-49	0.05856447866615963
50-51	0.06393659046868926
52-53	0.0668764469275539
54-55	0.06810314962261972
56-57	0.0719524580795504
58-59	0.05919898006015919
60-61	0.06926640217828559
62-63	0.06471914218795538
64-65	0.05888172936315941
66-67	0.06340783930702296
68-69	0.0659458448830212
70-71	0.06116593438155784
72-73	0.0567667247164942
74-75	0.060108432058225233
76-77	0.06937215241061885
78-79	0.06298483837768992
80-81	0.061271684613891104
82-83	0.06560744413955477
84-85	0.06723599771748699
86-87	0.06108133419569123
88-89	0.06036223261582505
90-91	0.06275218786655674
92-93	0.05767617671456024
94-95	0.06670724655582068
96-97	0.05310776667776339
98-99	0.0526636157019637
100-101	0.05056976110176514
102-103	0.04834900622276667
104-105	0.04667815255190116
106-107	0.057697326761026894
108-109	0.05763387662162694
110-111	0.06558629409308812
112-113	0.06554399400015483
114-115	0.0669610471134205
116-117	0.07095840589561775
118-119	0.06400004060808921
120-121	0.06550169390722152
122-123	0.06404234070102252
124-125	0.07550566588594795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2364061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.66502995880427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75892408228003	41.95645536975044
2	13.915456891467587	16.048704770738556
3	5.6505242325794205	9.775129474639252
4	2.8011674262579342	6.461176142191618
5	1.5483063570987865	4.464156623375431
6	0.9342159716973691	3.232295519755334
7	0.6002405654122797	2.4229023130892107
8	0.40714115566401027	1.8782245551061876
9	0.281298937530008	1.459900049404295
>10	1.0618639865863095	9.710131881192707
>50	0.03009427160109104	1.1767388888390355
>100	0.009883121821995022	0.9402206321969707
>500	6.62250002366029E-4	0.254675971879764
>1k	2.2075000078625195E-4	0.2192878078413188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.230009293330418E-5	0.0	0.0	0.0	0.0
6	1.269002787999125E-4	0.0	0.0	0.0	0.0
7	1.269002787999125E-4	0.0	0.0	0.0	0.0
8	2.1150046466652088E-4	0.0	0.0	0.0	0.0
9	2.1150046466652088E-4	0.0	0.0	0.0	0.0
10-11	2.1150046466652088E-4	0.0	0.0	0.0	0.0
12-13	2.749506040664771E-4	0.0	0.0	4.230009293330418E-5	0.0
14-15	3.172506969997813E-4	0.0	0.0	4.230009293330418E-5	0.0
16-17	3.807008363997376E-4	0.0	0.0	4.230009293330418E-5	0.0
18-19	5.0760111519965E-4	0.0	0.0	8.460018586660836E-5	0.0
20-21	5.922013010662584E-4	0.0	0.0	1.692003717332167E-4	0.0
22-23	6.768014869328668E-4	0.0	0.0	3.8070083639973754E-4	0.0
24-25	8.883019515993877E-4	0.0	0.0	5.499012081329542E-4	0.0
26-27	9.306020445326919E-4	0.0	0.0	0.0010786523697992564	0.0
28-29	0.001226702695065821	0.0	0.0	0.003616657945797507	0.0
30-31	0.0013747530203323858	0.0	0.0	0.013705230110390553	0.0
32-33	0.00141705311326569	0.0	0.0	0.0380066335005738	0.0
34-35	0.0015016532991322983	0.0	0.0	0.07798022132254626	0.0
36-37	0.001692003717332167	0.0	0.0	0.14365111560150098	0.0
38-39	0.0017766039031987754	0.0	0.0	0.24531938896669758	0.0
40-41	0.0017977539496654275	0.0	0.0	0.36062944230288474	0.0
42-43	0.0019035041819986877	0.0	0.0	0.4791120026090697	0.0
44-45	0.0021150046466652086	0.0	0.0	0.6222343670489043	0.0
46-47	0.0023688052042650336	0.0	0.0	0.7976317023968502	0.0
48-49	0.0025380055759982503	0.0	0.0	0.9828003592123892	0.0
50-51	0.0025380055759982503	0.0	0.0	1.1757310830811896	0.0
52-53	0.0025380055759982503	0.0	0.0	1.3654892999799921	2.115004646665209E-5
54-55	0.0025380055759982503	0.0	0.0	1.5857247338372402	4.230009293330418E-5
56-57	0.0026226057618648586	0.0	0.0	1.850058014577458	4.230009293330418E-5
58-59	0.0026226057618648586	0.0	0.0	2.138798448940192	4.230009293330418E-5
60-61	0.002664905854798163	0.0	0.0	2.439256009045452	4.230009293330418E-5
62-63	0.0027495060406647715	0.0	0.0	2.739798169336578	4.230009293330418E-5
64-65	0.0027918061335980757	0.0	0.0	3.0625901785106224	4.230009293330418E-5
66-67	0.002876406319464684	2.115004646665209E-5	0.0	3.4394628564998957	4.230009293330418E-5
68-69	0.002876406319464684	4.230009293330418E-5	0.0	3.8640500393179362	4.230009293330418E-5
70-71	0.0029821565517979447	4.230009293330418E-5	0.0	4.331212265673347	4.230009293330418E-5
72-73	0.003087906784131205	4.230009293330418E-5	0.0	4.79166992729883	4.230009293330418E-5
74-75	0.003130206877064509	4.230009293330418E-5	0.0	5.259064804165375	4.230009293330418E-5
76-77	0.0031725069699978133	4.230009293330418E-5	0.0	5.78804861634281	4.230009293330418E-5
78-79	0.0031725069699978133	4.230009293330418E-5	0.0	6.3808421187101345	4.230009293330418E-5
80-81	0.0034686076205309425	4.230009293330418E-5	0.0	7.014624411129832	4.230009293330418E-5
82-83	0.0035109077134642466	4.230009293330418E-5	0.0	7.656697521764455	4.230009293330418E-5
84-85	0.0037224081781307675	4.230009293330418E-5	0.0	8.297395879378747	4.230009293330418E-5
86-87	0.003891608549863984	4.230009293330418E-5	0.0	8.992449856412335	4.230009293330418E-5
88-89	0.003933908642797288	4.230009293330418E-5	0.0	9.757954638226341	4.230009293330418E-5
90-91	0.004272309386263722	4.230009293330418E-5	0.0	10.560873852239853	4.230009293330418E-5
92-93	0.004399209665063634	8.460018586660836E-5	0.0	11.370666831355027	4.230009293330418E-5
94-95	0.004399209665063634	8.460018586660836E-5	0.0	12.183759217719	4.230009293330418E-5
96-97	0.0044203597115302865	8.460018586660836E-5	0.0	13.020708856497357	4.230009293330418E-5
98-99	0.00446265980446359	8.460018586660836E-5	0.0	13.92256798788187	4.230009293330418E-5
100-101	0.004526109943863547	8.460018586660836E-5	0.0	14.8948779240468	4.230009293330418E-5
102-103	0.004589560083263503	8.460018586660836E-5	0.0	15.876197780006523	4.230009293330418E-5
104-105	0.004991410966129892	8.460018586660836E-5	0.0	16.84377010576292	4.230009293330418E-5
106-107	0.005012561012596544	8.460018586660836E-5	0.0	17.819083348526117	4.230009293330418E-5
108-109	0.005033711059063197	8.460018586660836E-5	0.0	18.84589695443561	4.230009293330418E-5
110-111	0.0050760111519965005	8.460018586660836E-5	0.0	19.91718910806447	4.230009293330418E-5
112-113	0.0050760111519965005	8.460018586660836E-5	0.0	21.025070842080638	4.230009293330418E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	205	2.4228939E-9	29.031506	7
GTATCAA	5560	0.0	27.439005	1
GTACATG	8190	0.0	24.885445	1
TCAACGC	6015	0.0	24.060623	4
TACATGG	8205	0.0	23.809982	2
ATCAACG	6275	0.0	23.444828	3
CATGGGG	4430	0.0	23.392775	4
ACCGTAT	255	2.4781912E-8	23.336088	8
CAACGCA	6165	0.0	23.185389	5
AACGCAG	6220	0.0	23.06293	6
ACATGGG	8295	0.0	22.330982	3
TATCAAC	6810	0.0	22.12775	2
GTATATA	1350	0.0	22.071886	1
GTGTACG	300	5.68798E-9	21.851166	1
GTATAGG	535	0.0	21.164255	1
GGTATCA	4285	0.0	21.139559	1
ACGCAGA	6920	0.0	20.468891	7
CGCAGAG	7040	0.0	20.117434	8
GTGTAGC	1910	0.0	19.032667	1
GTAGCCC	1790	0.0	18.966433	3
>>END_MODULE