FastQCFastQC Report
Mon 15 Aug 2016
SRR2920943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364061
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.18688181057933784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.1551144407864264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.11256054729552241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.034.454881
GTATCAA75150.034.371511
TCAACGC89500.028.0102064
ATCAACG90300.027.9598873
TATCAAC92200.027.3848652
CAACGCA91850.027.2935625
AACGCAG95100.026.5340526
GTACATG86800.026.528171
TACATGG87850.024.8771132
CATGGGG47950.024.8369734
ACATGGG87050.024.3522153
ACGCAGA109050.023.0797887
CGCAGAG110000.022.8790118
GAGTACT63650.022.668912-13
GCAGAGT123200.019.7515959
GTACTTT73250.019.57611714-15
AGAGTAC108000.019.36505310-11
AGTACTT71150.019.35945912-13
CAGAGTA116400.018.32539210-11
GCGTAGA6350.017.8492361
Produced by FastQC (version 0.11.5)