##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2364061
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187792531580193	31.0	31.0	34.0	28.0	34.0
2	31.28447658499506	33.0	31.0	34.0	28.0	34.0
3	31.37897372360527	33.0	31.0	34.0	28.0	34.0
4	34.93589336315772	37.0	35.0	37.0	32.0	37.0
5	34.67128555481436	37.0	35.0	37.0	32.0	37.0
6	34.38930425230144	37.0	35.0	37.0	30.0	37.0
7	34.28060062747958	37.0	35.0	37.0	30.0	37.0
8	34.28125500991726	37.0	35.0	37.0	30.0	37.0
9	35.83667172716778	39.0	37.0	39.0	30.0	39.0
10-11	35.81827203274366	39.0	36.0	39.0	30.0	39.0
12-13	35.73287639362944	39.0	35.0	39.0	30.0	39.0
14-15	36.95776356870656	40.0	37.0	41.0	30.0	41.0
16-17	36.97341608359514	40.0	37.0	41.0	30.0	41.0
18-19	37.015900393433164	40.0	37.0	41.0	30.0	41.0
20-21	37.009070408927684	40.0	37.0	41.0	30.0	41.0
22-23	36.962661496467305	40.0	37.0	41.0	30.0	41.0
24-25	36.95649160491206	40.0	37.0	41.0	30.0	41.0
26-27	36.843299728729505	40.0	37.0	41.0	30.0	41.0
28-29	36.745491338844474	40.0	37.0	41.0	30.0	41.0
30-31	36.608823757085794	40.0	36.0	41.0	30.0	41.0
32-33	36.471944463361986	40.0	36.0	41.0	30.0	41.0
34-35	36.366581699879994	40.0	36.0	41.0	29.5	41.0
36-37	36.24829139349619	40.0	36.0	41.0	29.0	41.0
38-39	36.12998818558404	40.0	35.0	41.0	28.0	41.0
40-41	36.00443051173383	40.0	35.0	41.0	27.5	41.0
42-43	35.883773303649946	40.0	35.0	41.0	27.0	41.0
44-45	35.70808875067098	40.0	35.0	41.0	26.5	41.0
46-47	35.524078481900425	40.0	35.0	41.0	26.0	41.0
48-49	35.29619963275059	39.5	35.0	41.0	24.5	41.0
50-51	35.13102073085255	39.0	35.0	41.0	24.0	41.0
52-53	34.95337408806287	39.0	35.0	41.0	24.0	41.0
54-55	34.7704502971793	39.0	34.0	41.0	23.5	41.0
56-57	34.54092745491762	39.0	34.0	41.0	23.0	41.0
58-59	34.32902683137195	38.0	34.0	41.0	22.5	41.0
60-61	34.07874775650882	38.0	34.0	40.0	22.0	41.0
62-63	33.796895257778885	37.5	33.5	40.0	22.0	41.0
64-65	33.50157737046548	37.0	33.0	40.0	20.5	41.0
66-67	33.18938280357402	36.5	33.0	39.5	20.0	41.0
68-69	32.82025654160362	36.0	32.5	39.0	19.0	41.0
70-71	32.47654417546755	35.5	32.0	39.0	18.0	40.5
72-73	32.1019956760845	35.0	32.0	38.5	17.5	40.0
74-75	31.7575819744076	35.0	32.0	37.0	15.0	39.5
76-77	31.51897984019871	35.0	32.0	37.0	14.0	39.0
78-79	31.349156599597052	35.0	32.0	36.5	13.0	39.0
80-81	31.078012369393175	35.0	32.0	36.0	12.0	38.0
82-83	30.827388548772642	35.0	32.0	36.0	10.5	37.0
84-85	30.566099182719903	35.0	31.0	35.0	7.5	37.0
86-87	30.38612709232122	35.0	31.0	35.0	7.0	36.0
88-89	30.22491403563614	35.0	31.0	35.0	7.0	36.0
90-91	30.05942295905224	35.0	31.0	35.0	3.5	36.0
92-93	29.928080324492473	35.0	31.0	35.0	2.0	35.5
94-95	29.82996377843042	35.0	31.0	35.0	2.0	35.0
96-97	29.767116838355694	35.0	31.0	35.0	2.0	35.0
98-99	29.66524277503838	35.0	31.0	35.0	2.0	35.0
100-101	29.571464949508496	34.5	31.0	35.0	2.0	35.0
102-103	28.182270677448678	33.0	28.0	34.5	2.0	35.0
104-105	29.237270738783813	34.0	30.0	35.0	2.0	35.0
106-107	29.40630614015459	34.0	30.5	35.0	2.0	35.0
108-109	29.49752036855225	34.5	31.0	35.0	2.0	35.0
110-111	29.663380936447915	35.0	31.0	35.0	2.0	35.0
112-113	29.6390725535424	35.0	31.0	35.0	2.0	35.0
114-115	29.556768628220674	35.0	31.0	35.0	2.0	35.0
116-117	29.44529434731168	35.0	31.0	35.0	2.0	35.0
118-119	29.34694070922874	35.0	31.0	35.0	2.0	35.0
120-121	29.16700859241788	35.0	31.0	35.0	2.0	35.0
122-123	28.98052334520979	35.0	31.0	35.0	2.0	35.0
124-125	28.269193349917792	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40633.0
3	16295.0
4	6451.0
5	5441.0
6	6125.0
7	7327.0
8	8337.0
9	9465.0
10	10983.0
11	12414.0
12	13607.0
13	15254.0
14	18202.0
15	16861.0
16	11025.0
17	9794.0
18	8998.0
19	8624.0
20	8571.0
21	8940.0
22	9367.0
23	11081.0
24	14342.0
25	19132.0
26	24496.0
27	29089.0
28	35313.0
29	42116.0
30	50658.0
31	61618.0
32	76423.0
33	98104.0
34	131001.0
35	187792.0
36	345451.0
37	677283.0
38	306539.0
39	909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.866923279099936	19.234741027749752	12.58243109193112	25.31590460121919
2	16.189197021005675	21.075771452502977	38.210784365567704	24.524247160923647
3	18.963944833514095	24.66866098938937	30.132391782894057	26.23500239420248
4	12.950268717705757	16.730548370244563	36.98909266266357	33.33009024938611
5	13.265985226429855	37.889618197028355	35.13667753855116	13.707719037990632
6	32.46603079152573	37.177449118138014	16.8431998943594	13.513320195976855
7	27.425688657997522	32.60466590307781	21.74298817678422	18.22665726214045
8	25.830089073649276	35.765181316110336	20.16967739042215	18.235052219818233
9	26.213562249045257	15.06779770726096	19.70691997327944	39.01172007041434
10-11	24.88361305192183	26.137708990430347	28.463776895807957	20.514901061839865
12-13	25.49113966570731	23.61785596282497	28.31742702339558	22.573577348072142
14-15	22.718703168882687	25.208669516057654	25.877050838020498	26.19557647703916
16-17	22.515379863590976	27.933809467395537	26.696735322490568	22.854075346522922
18-19	22.34000670616322	27.112362082041553	28.576251931724066	21.971379280071165
20-21	23.311930741146814	26.376126460106587	28.303819361078364	22.008123437668235
22-23	22.801010024730246	26.311220374628046	28.096722750242257	22.791046850399447
24-25	22.488544728967717	26.393194060815844	28.442070447960223	22.67619076225622
26-27	22.52778667952749	26.518407584502285	28.38853652061804	22.56526921535219
28-29	22.440697455753263	26.659645205377743	28.45049859214695	22.44915874672204
30-31	22.782129401987348	26.584305089528343	28.27122547571087	22.362340032773435
32-33	22.494850236980056	26.60981655463191	28.263790555573653	22.631542652814378
34-35	22.610857571010147	26.62851507483581	28.352655923636455	22.407971430517577
36-37	22.80326806289722	26.41969756329534	28.260320476966932	22.5167138968405
38-39	22.697099043436815	26.58243754478238	28.22507677658706	22.495386635193746
40-41	22.75045594756386	26.528204437135084	28.23671997848939	22.484619636811665
42-43	22.720779172700183	26.443829310576433	28.184782735164422	22.650608781558958
44-45	22.7581157574037	26.57480323289111	27.967964711173426	22.699116298531763
46-47	22.68140815263632	26.589718388934745	27.793084132157208	22.935789326271728
48-49	22.704235053161074	26.683980515468125	27.788706172337037	22.823078259033768
50-51	22.59909700663453	26.894640338385596	27.573768008887782	22.932494646092092
52-53	22.661399584344707	26.91747708872072	27.476168567787244	22.944954759147326
54-55	22.63107013817788	26.787935454937767	27.419128286272752	23.161866120611606
56-57	22.600143768190506	26.985898616770616	27.306436524736128	23.107521090302754
58-59	22.59829435898846	26.790473541198857	27.524916679976013	23.08631541983667
60-61	22.654042351730652	26.859789802533186	27.533638490988483	22.95252935474768
62-63	22.422021966453727	26.86198232901276	27.58675767594615	23.129238028587363
64-65	22.321615747082063	26.785799279704264	27.56175134460492	23.33083362860875
66-67	22.374952586878923	26.873915685984567	27.4078408450737	23.34329088206281
68-69	22.550364305121086	26.718508189355745	27.366439083496147	23.364688422027026
70-71	22.260950023015273	26.535317441514135	27.63436010776562	23.56937242770497
72-73	22.4243175580219	26.484782274808005	27.53631221331767	23.554587953852423
74-75	22.34079098836951	26.600487963579152	27.400441087937942	23.658279960113397
76-77	22.23170108212009	26.507151062966116	27.527018099921758	23.734129754992036
78-79	22.274669178028294	26.47927492048342	27.50867511289704	23.737380788591242
80-81	22.216270930442867	26.525676051585855	27.452935259605717	23.805117758365565
82-83	22.16085537163598	26.484842164624816	27.489434666056333	23.86486779768287
84-85	22.242139527563587	26.41898460947462	27.27978873459911	24.059087128362684
86-87	22.071769344039694	26.404808004936452	27.36588860874206	24.157534042281792
88-89	22.051164239989998	26.335137554690924	27.46949757438103	24.144200630938048
90-91	22.083376256303332	26.27099409175454	27.477244127450234	24.1683855244919
92-93	22.003774552358905	26.219262485806293	27.402691684815995	24.374271277018806
94-95	21.860527887251603	26.301457605441964	27.428522715710567	24.409491791595865
96-97	21.863439070829436	26.195588737062387	27.4585876927249	24.482384499383276
98-99	21.806642645280103	26.164083246500127	27.378542445891597	24.65073166232818
100-101	21.742203279543006	26.192132244495948	27.352364295136873	24.713300180824177
102-103	21.66638318685466	26.10859646183346	27.37668205073527	24.848338300576607
104-105	21.55805527688041	26.168833352687244	27.366554794724824	24.906556575707523
106-107	21.44256192228779	26.199145461852485	27.386297776931702	24.97199483892802
108-109	21.411293708815833	26.208483195706382	27.280934756217647	25.09928833926014
110-111	21.343847097747084	26.329001037965593	27.22168952538933	25.105462338897986
112-113	21.28517003942137	26.393893442371553	27.160174454944634	25.160762063262442
114-115	21.18152830877104	26.35487094880784	27.20988202695771	25.253718715463403
116-117	21.092624863602204	26.349061179183398	27.27336180404869	25.284952153165708
118-119	21.127772810923158	26.327276449769872	27.14571820823522	25.399232531071746
120-121	21.0474274024616	26.4810329307375	27.103814226105673	25.36772544069523
122-123	20.940033100501797	26.483806663458843	27.076273919871408	25.49988631616796
124-125	20.86361778029888	26.514211773175916	27.003098716246125	25.619071730279074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	62.0
2	163.0
3	310.0
4	238.0
5	135.5
6	266.5
7	611.0
8	919.0
9	982.5
10	840.0
11	660.0
12	691.5
13	826.5
14	932.5
15	1068.0
16	1206.5
17	1368.0
18	1597.5
19	1823.0
20	2172.0
21	2744.0
22	3374.5
23	4215.5
24	5421.5
25	6935.5
26	9276.5
27	12420.5
28	15293.5
29	18733.0
30	23143.0
31	27472.0
32	32378.0
33	37290.0
34	41649.5
35	46123.5
36	51641.5
37	56964.5
38	60975.5
39	65167.0
40	69920.5
41	74564.0
42	78328.0
43	83359.0
44	88429.0
45	91538.5
46	94329.5
47	96795.0
48	99357.5
49	101473.5
50	103498.0
51	100968.0
52	95106.0
53	90310.5
54	84567.5
55	78155.0
56	70855.5
57	63065.0
58	55774.5
59	48310.0
60	39543.0
61	30770.0
62	23387.5
63	17480.0
64	12753.5
65	9278.0
66	6759.0
67	4945.0
68	3548.5
69	2460.5
70	1801.0
71	1301.5
72	906.0
73	691.5
74	520.5
75	368.5
76	242.0
77	151.5
78	92.5
79	49.0
80	38.5
81	30.0
82	21.5
83	16.5
84	11.5
85	10.5
86	8.5
87	3.5
88	2.5
89	4.0
90	2.5
91	1.5
92	3.0
93	5.0
94	5.5
95	6.5
96	8.0
97	6.0
98	2.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21661877591145068
2	0.007444816356261534
3	7.19101579866171E-4
4	0.002368805204265034
5	0.08481168633127487
6	0.05638602388009446
7	0.027918061335980758
8	0.016877737080388366
9	0.01429743141145681
10-11	0.012118976625391647
12-13	0.013705230110390553
14-15	0.02178454786065165
16-17	0.024576353994249726
18-19	0.023836102367916903
20-21	0.017194987777388145
22-23	0.015079983130722938
24-25	0.01277462806585786
26-27	0.012415077275924774
28-29	0.015037683037789636
30-31	0.01084997383739252
32-33	0.015228033455989503
34-35	0.017766039031987754
36-37	0.019415742656386615
38-39	0.023159300880984036
40-41	0.024386003576049857
42-43	0.022355599115251257
44-45	0.020304044607986006
46-47	0.02038864479385261
48-49	0.018717791122987097
50-51	0.015524134106522633
52-53	0.013684080063923902
54-55	0.01865434098358714
56-57	0.022588249626384428
58-59	0.01833709028658736
60-61	0.02005024405038618
62-63	0.027029759384381367
64-65	0.018971591680586923
66-67	0.02231329902231795
68-69	0.0162009355934555
70-71	0.017469938381454622
72-73	0.025380055759982503
74-75	0.015228033455989503
76-77	0.013599479878057293
78-79	0.01704693745212158
80-81	0.018125589821920838
82-83	0.013387979413390773
84-85	0.016877737080388366
86-87	0.0177448889855211
88-89	0.018506290658320577
90-91	0.01398018071445703
92-93	0.014255131318523509
94-95	0.015037683037789636
96-97	0.01529148359538946
98-99	0.014128231039723594
100-101	0.018569740797720533
102-103	0.024407153622516508
104-105	0.008354268354327574
106-107	0.00892531960892718
108-109	0.015418383874189371
110-111	0.012795778112324513
112-113	0.010215472443392958
114-115	0.007339066123928275
116-117	0.004716460362063415
118-119	0.002389955250731686
120-121	0.002918706412397988
122-123	0.0020515545072652525
124-125	7.40251626332823E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2364061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.44618430984386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3096744696373	38.28093494938996
2	14.956305466280368	16.286275280228523
3	6.150795330173898	10.046620085963246
4	3.0575814808925834	6.658945794041717
5	1.704438146346526	4.64000767303558
6	1.0657385051413124	3.4815237046212824
7	0.6874742558792819	2.6201245030713296
8	0.4542813811019693	1.9787110243206594
9	0.3296698580278143	1.615433926642419
>10	1.2307413797012416	10.698450794574859
>50	0.03749380977292116	1.3808901328841425
>100	0.014640155344436582	1.3883211744469486
>500	6.21739573571204E-4	0.22443443336384664
>1k	5.440221268731866E-4	0.6993265234154341
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4418	0.18688181057933784	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3667	0.1551144407864264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2661	0.11256054729552241	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.230009293330418E-5	0.0	0.0	0.0	0.0
3	4.230009293330418E-5	0.0	0.0	0.0	0.0
4	4.230009293330418E-5	0.0	0.0	0.0	0.0
5	4.230009293330418E-5	0.0	0.0	0.0	0.0
6	4.230009293330418E-5	0.0	0.0	0.0	0.0
7	8.460018586660836E-5	0.0	0.0	0.0	0.0
8	8.460018586660836E-5	0.0	0.0	0.0	0.0
9	8.460018586660836E-5	0.0	0.0	0.0	0.0
10-11	8.460018586660836E-5	0.0	0.0	0.0	0.0
12-13	1.903504181998688E-4	0.0	0.0	4.230009293330418E-5	0.0
14-15	2.53800557599825E-4	0.0	0.0	4.230009293330418E-5	0.0
16-17	2.53800557599825E-4	0.0	0.0	4.230009293330418E-5	0.0
18-19	3.5955078993308545E-4	0.0	0.0	8.460018586660836E-5	0.0
20-21	4.6530102226634594E-4	0.0	0.0	1.692003717332167E-4	0.0
22-23	4.8645106873299797E-4	0.0	0.0	3.5955078993308545E-4	0.0
24-25	7.19101579866171E-4	0.0	0.0	5.499012081329542E-4	0.0
26-27	7.19101579866171E-4	0.0	0.0	0.0010786523697992564	0.0
28-29	0.0010575023233326043	0.0	0.0	0.003595507899330855	0.0
30-31	0.001226702695065821	0.0	0.0	0.013895580528590423	0.0
32-33	0.0012690027879991251	0.0	0.0	0.03836618429050689	0.0
34-35	0.0016074035314655587	0.0	0.0	0.07855127257714586	0.0
36-37	0.0018612040890653837	0.0	0.0	0.14468746787836695	0.0
38-39	0.001988104367865296	0.0	0.0	0.24751899379922937	0.0
40-41	0.001988104367865296	0.0	0.0	0.3636961990405493	0.0
42-43	0.002178454786065165	0.0	0.0	0.4834477621347334	0.0
44-45	0.0023688052042650336	0.0	0.0	0.6281775301060337	0.0
46-47	0.002707205947731467	0.0	0.0	0.8039132661974457	0.0
48-49	0.002876406319464684	0.0	0.0	0.9902663256151174	0.0
50-51	0.002876406319464684	0.0	0.0	1.1847410028759833	0.0
52-53	0.002876406319464684	0.0	0.0	1.377460226280117	0.0
54-55	0.002876406319464684	0.0	0.0	1.5998106647840307	0.0
56-57	0.003087906784131205	0.0	0.0	1.8665550508214466	0.0
58-59	0.003087906784131205	0.0	0.0	2.159694694849245	0.0
60-61	0.003087906784131205	0.0	0.0	2.464889865363034	0.0
62-63	0.003087906784131205	0.0	0.0	2.7697466351333575	0.0
64-65	0.003087906784131205	0.0	0.0	3.0969801540653985	0.0
66-67	0.0031725069699978133	0.0	0.0	3.479584494647135	0.0
68-69	0.0031725069699978133	0.0	0.0	3.909247688617172	0.0
70-71	0.003193657016464465	2.115004646665209E-5	0.0	4.378990220641515	0.0
72-73	0.0032148070629311174	4.230009293330418E-5	0.0	4.841880137610662	0.0
74-75	0.0032359571093977697	4.230009293330418E-5	0.0	5.313018572701804	0.0
76-77	0.0032571071558644216	4.230009293330418E-5	0.0	5.847903247843435	0.0
78-79	0.0032571071558644216	4.230009293330418E-5	0.0	6.447105214290156	0.0
80-81	0.0036589580387308114	4.230009293330418E-5	0.0	7.085857767629515	0.0
82-83	0.0036801080851974633	4.230009293330418E-5	0.0	7.734170141971802	0.0
84-85	0.0037224081781307675	4.230009293330418E-5	0.0	8.379606109994624	0.0
86-87	0.003828158410464028	4.230009293330418E-5	0.0	9.07958804785494	0.0
88-89	0.00384930845693068	4.230009293330418E-5	0.0	9.851628194027143	0.0
90-91	0.004208859246863765	4.230009293330418E-5	0.0	10.664403329694116	0.0
92-93	0.004272309386263722	4.230009293330418E-5	0.0	11.483481179208152	0.0
94-95	0.004272309386263722	4.230009293330418E-5	0.0	12.30568923559925	0.0
96-97	0.004272309386263722	4.230009293330418E-5	0.0	13.152748596588665	0.0
98-99	0.004399209665063634	4.230009293330418E-5	0.0	14.067022805249103	0.0
100-101	0.0045684100367968505	4.230009293330418E-5	0.0	15.05305912157089	0.0
102-103	0.004695310315596763	4.230009293330418E-5	0.0	16.049585860940137	0.0
104-105	0.005139461291396457	4.230009293330418E-5	0.0	17.03354947270819	0.0
106-107	0.005329811709596326	4.230009293330418E-5	0.0	18.02487330064664	0.0
108-109	0.0054567119883962385	4.230009293330418E-5	0.0	19.071969801117653	0.0
110-111	0.00547786203486289	4.230009293330418E-5	0.0	20.16885351097116	0.0
112-113	0.005499012081329543	4.230009293330418E-5	0.0	21.300169496472385	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5575	0.0	34.45488	1
GTATCAA	7515	0.0	34.37151	1
TCAACGC	8950	0.0	28.010206	4
ATCAACG	9030	0.0	27.959887	3
TATCAAC	9220	0.0	27.384865	2
CAACGCA	9185	0.0	27.293562	5
AACGCAG	9510	0.0	26.534052	6
GTACATG	8680	0.0	26.52817	1
TACATGG	8785	0.0	24.877113	2
CATGGGG	4795	0.0	24.836973	4
ACATGGG	8705	0.0	24.352215	3
ACGCAGA	10905	0.0	23.079788	7
CGCAGAG	11000	0.0	22.879011	8
GAGTACT	6365	0.0	22.6689	12-13
GCAGAGT	12320	0.0	19.751595	9
GTACTTT	7325	0.0	19.576117	14-15
AGAGTAC	10800	0.0	19.365053	10-11
AGTACTT	7115	0.0	19.359459	12-13
CAGAGTA	11640	0.0	18.325392	10-11
GCGTAGA	635	0.0	17.849236	1
>>END_MODULE