FastQCFastQC Report
Mon 15 Aug 2016
SRR2920942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052765
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22500.10960826007847951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81550.026.3089711
CATGGGG45900.025.695394
TACATGG82450.025.2162132
ACATGGG84500.023.6174623
GTATCAA63350.023.613071
GCGTACA2052.240744E-623.2477682
AACGCAG64400.022.5543966
TCAACGC66450.021.6035334
CAACGCA68300.021.1055835
ATCAACG68050.021.0976473
TATCAAC70250.020.860942
GGTATCA49600.020.7869011
ACGCAGA72050.019.826757
CGCAGAG72600.019.264278
CGTACAC3101.9293293E-719.2169043
GTATTAC5950.019.0310151
ATACCGT2201.0181771E-418.9409876
ATTATCG1600.006786646318.6163773
ATGGGGA29900.018.3281825
GAGTACT22450.017.75739312-13
Produced by FastQC (version 0.11.5)