##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920942_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2052765
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.86692680360392	31.0	30.0	34.0	25.0	34.0
2	29.907933933012302	31.0	30.0	34.0	25.0	34.0
3	30.04440206258388	31.0	31.0	34.0	25.0	34.0
4	33.450117280838285	37.0	35.0	37.0	27.0	37.0
5	33.54768373389063	37.0	35.0	37.0	28.0	37.0
6	33.51629826112585	37.0	35.0	37.0	28.0	37.0
7	33.46512533095605	37.0	35.0	37.0	27.0	37.0
8	33.374970832024125	37.0	35.0	37.0	27.0	37.0
9	34.7938877562702	39.0	35.0	39.0	27.0	39.0
10-11	34.80544826124763	39.0	35.0	39.0	27.0	39.0
12-13	34.75192752214696	39.0	35.0	39.0	27.0	39.0
14-15	35.92164860566115	40.0	36.0	41.0	27.0	41.0
16-17	35.91180675820174	40.0	36.0	41.0	27.0	41.0
18-19	35.89817514425665	40.0	36.0	41.0	27.0	41.0
20-21	35.926140352159166	40.0	36.0	41.0	27.0	41.0
22-23	35.853185094250925	40.0	36.0	41.0	27.0	41.0
24-25	35.86014180873115	40.0	36.0	41.0	27.0	41.0
26-27	35.790048057132694	40.0	36.0	41.0	27.0	41.0
28-29	35.682778593750385	40.0	36.0	41.0	26.5	41.0
30-31	35.537634361458814	40.0	35.0	41.0	26.0	41.0
32-33	35.40570523172404	40.0	35.0	41.0	25.0	41.0
34-35	35.339542763053736	40.0	35.0	41.0	25.0	41.0
36-37	35.20635874052802	40.0	35.0	41.0	25.0	41.0
38-39	35.06540032590189	40.0	35.0	41.0	24.0	41.0
40-41	34.94020966842284	39.0	35.0	41.0	24.0	41.0
42-43	34.76375011265293	39.0	34.5	41.0	23.0	41.0
44-45	34.60792906153408	39.0	34.0	41.0	23.0	41.0
46-47	34.41943451880756	39.0	34.0	41.0	22.0	41.0
48-49	34.29597810757686	39.0	34.0	41.0	21.0	41.0
50-51	34.10862738793773	39.0	33.0	40.5	19.5	41.0
52-53	33.921312473663576	38.0	33.0	40.0	18.5	41.0
54-55	33.68852279730022	38.0	33.0	40.0	17.5	41.0
56-57	33.42631937898396	38.0	33.0	40.0	14.5	41.0
58-59	33.1720026403412	38.0	32.0	40.0	13.0	41.0
60-61	32.905617788689895	37.0	32.0	40.0	10.5	41.0
62-63	32.59737865756675	37.0	32.0	40.0	8.0	41.0
64-65	32.310040603771014	36.0	31.0	39.5	7.5	41.0
66-67	31.956467252705497	36.0	31.0	39.0	7.0	41.0
68-69	31.630999408115397	35.0	31.0	39.0	6.5	40.0
70-71	31.2806616928874	35.0	31.0	38.0	2.0	40.0
72-73	31.328314980039117	35.0	31.0	38.0	2.0	40.0
74-75	31.304742871200553	35.0	31.0	37.0	2.0	39.5
76-77	31.02784342094687	35.0	31.0	37.0	2.0	39.0
78-79	30.72320918371075	35.0	31.0	36.0	2.0	39.0
80-81	30.43809081896856	35.0	31.0	36.0	2.0	37.5
82-83	30.18474569665792	35.0	31.0	35.5	2.0	37.0
84-85	29.940876330218025	35.0	31.0	35.0	2.0	37.0
86-87	29.732956524492575	35.0	31.0	35.0	2.0	36.0
88-89	29.52380447834993	35.0	30.5	35.0	2.0	36.0
90-91	29.341806538985225	35.0	30.0	35.0	2.0	36.0
92-93	29.164342484405182	34.5	30.0	35.0	2.0	35.5
94-95	29.04191955728006	34.0	30.0	35.0	2.0	35.0
96-97	28.930471583449638	34.0	30.0	35.0	2.0	35.0
98-99	28.78042177258478	34.0	30.0	35.0	2.0	35.0
100-101	28.638351686627548	34.0	29.0	35.0	2.0	35.0
102-103	27.26316991959625	32.5	26.0	34.5	2.0	35.0
104-105	28.26156062676439	34.0	29.0	35.0	2.0	35.0
106-107	28.39229259072519	34.0	29.0	35.0	2.0	35.0
108-109	28.296367582261	34.0	29.0	35.0	2.0	35.0
110-111	28.166948238108112	34.0	29.0	35.0	2.0	35.0
112-113	28.02258904453262	34.0	29.0	35.0	2.0	35.0
114-115	27.881814284635603	34.0	29.0	35.0	2.0	35.0
116-117	27.642044754270458	34.0	28.5	35.0	2.0	35.0
118-119	27.40445813329826	34.0	27.0	35.0	2.0	35.0
120-121	27.182711123776954	34.0	27.0	35.0	2.0	35.0
122-123	26.845636982314097	34.0	26.0	35.0	2.0	35.0
124-125	25.52290179343471	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73838.0
3	10692.0
4	7574.0
5	7417.0
6	10239.0
7	10663.0
8	9309.0
9	9873.0
10	10730.0
11	11216.0
12	11589.0
13	11427.0
14	11504.0
15	10584.0
16	8808.0
17	8193.0
18	7917.0
19	8001.0
20	8297.0
21	9006.0
22	10665.0
23	13687.0
24	18431.0
25	22956.0
26	26259.0
27	29012.0
28	33887.0
29	39921.0
30	47104.0
31	57468.0
32	72064.0
33	91659.0
34	123227.0
35	179079.0
36	315955.0
37	520471.0
38	193756.0
39	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47001426346966	18.284421294974702	11.798129206637809	25.44743523491783
2	16.090986130293956	21.188465345737637	38.041165642705174	24.679382881263237
3	19.1164851874824	24.60492714399279	29.266013364376658	27.012574304148153
4	13.015438581084874	16.77387564733978	36.065198672023456	34.14548709955189
5	13.770097577067144	37.969561576614296	34.20911630194822	14.051224544370337
6	32.73125400815896	36.4273191238256	16.93899795416251	13.90242891385293
7	27.834329580318055	32.72311681522183	21.298065035900493	18.144488568559623
8	25.443113833772763	36.093157158941686	20.068793639075256	18.394935368210298
9	25.901799551550702	16.377372328981156	19.36700181700967	38.35382630245847
10-11	24.66793682370537	26.837052991878064	27.884554670996415	20.61045551342015
12-13	25.093842759536567	24.435072559789642	27.571086850166772	22.899997830507022
14-15	22.681629421475034	25.95737482095689	24.881799859788163	26.479195897779917
16-17	22.815849804443026	28.45608103578772	25.852034852896548	22.876034306872707
18-19	22.907803613968262	27.76226815497294	27.08314086802807	22.24678736303072
20-21	23.644707121705625	27.07714484398243	26.76296234367958	22.515185690632364
22-23	23.283107959734238	27.002668022409733	26.449517053091625	23.264706964764407
24-25	23.015455964855924	27.101705581588746	26.710395278245873	23.172443175309454
26-27	22.98900390668324	27.11521412655879	26.691663069228998	23.204118897528968
28-29	22.968352050020147	27.329734536095668	26.765053074012584	22.9368603398716
30-31	23.202942387984763	27.283726439812906	26.57998581453109	22.933345357671243
32-33	22.849984021637944	27.375870308839435	26.596111615013857	23.17803405450876
34-35	22.987578083352446	27.391543756049654	26.65289785002195	22.967980310575946
36-37	23.146054324083664	27.220835924262875	26.530297848628614	23.102811903024847
38-39	22.98556088495946	27.298648712550023	26.68501435429091	23.03077604819961
40-41	23.06850786954081	27.22783849604494	26.746469133298707	22.95718450111555
42-43	22.929914879925118	27.294756291377816	26.613186298885545	23.16214252981153
44-45	22.925781541345923	27.29499849612185	26.67665352256037	23.102566439971863
46-47	22.81635640543985	27.368869266675	26.618607191664882	23.196167136220268
48-49	22.773115264858244	27.346572508710327	26.673142950728902	23.20716927570253
50-51	22.731770639111616	27.432478477491852	26.624954849163302	23.21079603423323
52-53	22.849557893115378	27.37472500849409	26.41897566913178	23.356741429258754
54-55	22.806056279359645	27.266223926539617	26.51029800991181	23.417421784188928
56-57	22.782010142237507	27.267360446736188	26.46209945047646	23.48852996054984
58-59	22.715751752052306	27.078749499169913	26.68327651572387	23.522222233053906
60-61	22.773501234052738	27.082570632030965	26.738264312013772	23.40566382190252
62-63	22.687973942644668	26.90391715812212	26.830067836573157	23.578041062660052
64-65	22.633792495176326	26.87730871580505	26.84962301383725	23.639275775181375
66-67	22.681647574945163	27.011162563977575	26.694296855959053	23.612893005118206
68-69	22.850718104980842	26.83386946054018	26.746443109989514	23.56896932448947
70-71	22.609609914074642	26.721992368689403	26.87709475966441	23.79130295757155
72-73	22.751343253682478	26.61948980852649	26.961752333436422	23.66741460435461
74-75	22.584061901905788	26.760744279316047	26.974068864870954	23.68112495390722
76-77	22.599965779084	26.571005719572753	27.09952875556273	23.729499745780515
78-79	22.641142928449497	26.555908792679705	27.130682787538106	23.67226549133269
80-81	22.57626920363192	26.54817800839129	27.1240158271926	23.751536960784193
82-83	22.60942959537031	26.482239931053847	27.16923979846509	23.73909067511075
84-85	22.672110070500203	26.45621005039211	27.025427601713453	23.846252277394235
86-87	22.569700686015214	26.291213799463627	27.164184488081737	23.97490102643942
88-89	22.695076944298826	26.239635130264137	27.111986533315886	23.95330139212115
90-91	22.637442450505606	26.29331292545193	27.1554646733236	23.913779950718865
92-93	22.68192387432276	26.236016846195483	27.1469796680234	23.935079611458356
94-95	22.559350934610876	26.173637860587824	27.257412103291244	24.00959910151006
96-97	22.670182152807158	26.05886189101246	27.204189339099006	24.066766617081385
98-99	22.67709224369238	26.168685738795183	27.04191920299656	24.112302814515875
100-101	22.53361247980668	26.30306465688354	27.174987163275098	23.98833570003468
102-103	22.4580224960382	26.306771645253995	27.152255586420303	24.0829502722875
104-105	22.331726709745524	26.39897242373046	27.146090163433072	24.12321070309094
106-107	22.278068177830118	26.52012404122489	27.025613479588372	24.176194301356617
108-109	22.303381931673115	26.542458272384344	27.11226328598606	24.04189650995648
110-111	22.283883651228614	26.7036667738274	27.07635701926773	23.936092555676257
112-113	22.285007051106753	26.849264252473006	26.960526963597957	23.90520173282228
114-115	22.265734967973973	26.87232024709238	26.957506866724014	23.904437918209634
116-117	22.179170428552762	26.894284338138796	26.97265019431807	23.953895038990364
118-119	22.41003081496228	26.886791877991957	26.78752007432833	23.915657232717432
120-121	22.34679477336375	26.99526911912022	26.90750043262821	23.75043567488782
122-123	22.347643716190607	27.066475097886528	26.81465720521623	23.771223980706637
124-125	22.51400123242046	27.106400591249812	26.58571757291171	23.793880603418017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2060.0
1	1454.5
2	682.0
3	476.0
4	396.5
5	331.0
6	310.5
7	337.5
8	371.0
9	390.5
10	379.5
11	383.5
12	440.5
13	506.0
14	566.5
15	666.5
16	828.5
17	975.5
18	1120.5
19	1416.0
20	1782.5
21	2244.0
22	2917.5
23	3638.0
24	4702.5
25	6111.0
26	7931.0
27	10343.5
28	12614.0
29	15127.5
30	17917.0
31	20935.0
32	25127.5
33	29042.5
34	32470.0
35	36186.0
36	40439.0
37	45348.5
38	49429.0
39	53453.0
40	58229.0
41	62539.5
42	66411.0
43	71001.0
44	75107.5
45	78740.5
46	82086.0
47	84237.5
48	86526.0
49	88229.5
50	89577.0
51	89090.0
52	85374.0
53	81397.0
54	77313.5
55	71290.5
56	64467.0
57	57473.5
58	50926.0
59	44545.0
60	37022.0
61	29832.5
62	23477.0
63	17620.5
64	12858.5
65	9416.0
66	6875.0
67	5193.0
68	3864.5
69	2762.0
70	2161.0
71	1663.5
72	1219.5
73	944.0
74	690.0
75	506.5
76	350.0
77	227.5
78	142.5
79	114.0
80	100.5
81	71.0
82	45.5
83	37.5
84	28.5
85	15.5
86	16.5
87	15.5
88	11.5
89	12.5
90	12.5
91	9.5
92	6.5
93	6.5
94	7.5
95	7.0
96	4.0
97	4.0
98	5.0
99	6.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16923515356117239
2	0.14838522675513272
3	0.1746912091739678
4	0.14760579023901907
5	0.17113503006919933
6	0.11009540790105053
7	0.10892625312688008
8	0.0971372758206614
9	0.10439577837696959
10-11	0.08951341239742494
12-13	0.07767572030894915
14-15	0.07711550031299247
16-17	0.07660399509929292
18-19	0.07450925946223752
20-21	0.06866348559138528
22-23	0.06067426130122055
24-25	0.06449837170840306
26-27	0.07485026293803723
28-29	0.06973521080104152
30-31	0.06673925169222975
32-33	0.07567841423640796
34-35	0.07363239338160968
36-37	0.07494769250255144
38-39	0.07122101165988313
40-41	0.07470411859126593
42-43	0.0752643385872226
44-45	0.06829812472445701
46-47	0.06569188387370205
48-49	0.05733729871660906
50-51	0.06371893519228942
52-53	0.06518037866000248
54-55	0.0669097534301296
56-57	0.07027107340586965
58-59	0.05704501002306645
60-61	0.0679084064664002
62-63	0.06286642650279013
64-65	0.05672836393839528
66-67	0.06162419955523404
68-69	0.06449837170840306
70-71	0.05928589000689314
72-73	0.05473105786585411
74-75	0.05828723697062255
76-77	0.06761611777285759
78-79	0.061526769990719835
80-81	0.06004096913187823
82-83	0.06376764997454652
84-85	0.06588674300273047
86-87	0.05879874218432213
88-89	0.05765394480128022
90-91	0.06186777346651955
92-93	0.05575406829325325
94-95	0.06444965692614595
96-97	0.05266067961992727
98-99	0.052611964837670167
100-101	0.05039544224497202
102-103	0.04781355878534562
104-105	0.04610854140634705
106-107	0.05792187610369428
108-109	0.057775731756922986
110-111	0.06476630301081712
112-113	0.06401122388583204
114-115	0.06754304559947193
116-117	0.07253631078082488
118-119	0.06462015866404582
120-121	0.06576495604708771
122-123	0.06247670824473333
124-125	0.07390032468402374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2052765.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.48177614218089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92951507525919	39.35295477278975
2	14.658196877910523	16.265255956564932
3	6.010356188676925	10.003957097848339
4	2.9885863313234373	6.632483112642744
5	1.6956282349729075	4.703823307656407
6	1.0517412248334774	3.501148271742848
7	0.6385811399088961	2.4800731097130853
8	0.45275656886381743	2.009579088048338
9	0.3026551335611034	1.5112659931667098
>10	1.228203700686316	10.781399798728593
>50	0.031528551065396836	1.1683888838490444
>100	0.011370306505120102	1.0629496968754204
>500	5.283998597016001E-4	0.1972516062318121
>1k	3.522665731343857E-4	0.3294693041419534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2250	0.10960826007847951	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	2.4357391128551002E-4
14-15	0.0	0.0	0.0	0.0	3.4100347579971407E-4
16-17	0.0	0.0	0.0	0.0	3.8971825805681604E-4
18-19	0.0	0.0	0.0	0.0	4.3843304031391807E-4
20-21	0.0	0.0	0.0	0.0	6.089347782137751E-4
22-23	0.0	0.0	0.0	0.0	6.332921693423261E-4
24-25	0.0	0.0	0.0	2.19216520156959E-4	6.332921693423261E-4
26-27	0.0	0.0	0.0	7.550791249850812E-4	6.332921693423261E-4
28-29	0.0	0.0	0.0	0.0034343921491256915	6.332921693423261E-4
30-31	0.0	0.0	0.0	0.014370860765845091	6.576495604708771E-4
32-33	0.0	0.0	0.0	0.04457402576524834	7.307217338565301E-4
34-35	0.0	0.0	0.0	0.09516432713924877	7.307217338565301E-4
36-37	0.0	0.0	0.0	0.18594432387535836	7.307217338565301E-4
38-39	0.0	0.0	0.0	0.33389111759017714	7.550791249850811E-4
40-41	0.0	0.0	0.0	0.5048069311392195	7.794365161136321E-4
42-43	0.0	0.0	0.0	0.6675873760513259	7.794365161136321E-4
44-45	2.4357391128551002E-5	0.0	0.0	0.8698511519828134	7.794365161136321E-4
46-47	4.8714782257102005E-5	0.0	0.0	1.1229000884173297	7.794365161136321E-4
48-49	4.8714782257102005E-5	0.0	0.0	1.386739349121794	8.768660806278361E-4
50-51	4.8714782257102005E-5	0.0	0.0	1.6546706515358554	9.742956451420401E-4
52-53	4.8714782257102005E-5	0.0	0.0	1.9268644973974127	0.001023010427399142
54-55	4.8714782257102005E-5	0.0	0.0	2.2390775368831797	0.001023010427399142
56-57	4.8714782257102005E-5	0.0	0.0	2.611209758545182	0.001023010427399142
58-59	4.8714782257102005E-5	0.0	0.0	3.0071878661220355	0.001023010427399142
60-61	4.8714782257102005E-5	0.0	0.0	3.4111308405979255	0.001023010427399142
62-63	4.8714782257102005E-5	0.0	0.0	3.8152199594205864	0.0010717252096562441
64-65	4.8714782257102005E-5	0.0	0.0	4.256722031016702	0.0010960826007847951
66-67	4.8714782257102005E-5	0.0	0.0	4.775899822921767	0.0011204399919133461
68-69	4.8714782257102005E-5	0.0	0.0	5.345740988374217	0.0011204399919133461
70-71	4.8714782257102005E-5	0.0	0.0	5.953165608338022	0.0011204399919133461
72-73	4.8714782257102005E-5	0.0	0.0	6.559615932656685	0.0012178695564275502
74-75	4.8714782257102005E-5	0.0	0.0	7.174981062128397	0.0013152991209417542
76-77	4.8714782257102005E-5	0.0	0.0	7.863369650203506	0.0013152991209417542
78-79	4.8714782257102005E-5	0.0	0.0	8.616621970853945	0.0013152991209417542
80-81	4.8714782257102005E-5	0.0	0.0	9.406069374721412	0.0013152991209417542
82-83	4.8714782257102005E-5	0.0	0.0	10.21531933757639	0.0013640139031988563
84-85	4.8714782257102005E-5	0.0	0.0	11.024398798693468	0.0013640139031988563
86-87	4.8714782257102005E-5	0.0	0.0	11.900996948018891	0.0014370860765845093
88-89	4.8714782257102005E-5	0.0	0.0	12.839584657766476	0.0015101582499701621
90-91	4.8714782257102005E-5	0.0	0.0	13.818824853307612	0.0015101582499701621
92-93	4.8714782257102005E-5	0.0	0.0	14.803618534026057	0.0015588730322272642
94-95	4.8714782257102005E-5	0.0	0.0	15.792723472974256	0.0015588730322272642
96-97	4.8714782257102005E-5	0.0	0.0	16.81517368037744	0.001826804334641325
98-99	4.8714782257102005E-5	0.0	0.0	17.90114796384389	0.0019485912902840801
100-101	4.8714782257102005E-5	0.0	0.0	19.040294432143963	0.0019485912902840801
102-103	4.8714782257102005E-5	2.4357391128551002E-5	0.0	20.203773934181456	0.0019485912902840801
104-105	4.8714782257102005E-5	4.8714782257102005E-5	0.0	21.34077695206222	0.0019485912902840801
106-107	4.8714782257102005E-5	7.307217338565301E-5	0.0	22.4897394489871	0.0019485912902840801
108-109	4.8714782257102005E-5	9.742956451420401E-5	0.0	23.680060796048256	0.0019485912902840801
110-111	4.8714782257102005E-5	9.742956451420401E-5	0.0	24.917123976685104	0.0019485912902840801
112-113	4.8714782257102005E-5	9.742956451420401E-5	0.0	26.184633896232643	0.0019485912902840801
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8155	0.0	26.308971	1
CATGGGG	4590	0.0	25.69539	4
TACATGG	8245	0.0	25.216213	2
ACATGGG	8450	0.0	23.617462	3
GTATCAA	6335	0.0	23.61307	1
GCGTACA	205	2.240744E-6	23.247768	2
AACGCAG	6440	0.0	22.554396	6
TCAACGC	6645	0.0	21.603533	4
CAACGCA	6830	0.0	21.105583	5
ATCAACG	6805	0.0	21.097647	3
TATCAAC	7025	0.0	20.86094	2
GGTATCA	4960	0.0	20.786901	1
ACGCAGA	7205	0.0	19.82675	7
CGCAGAG	7260	0.0	19.26427	8
CGTACAC	310	1.9293293E-7	19.216904	3
GTATTAC	595	0.0	19.031015	1
ATACCGT	220	1.0181771E-4	18.940987	6
ATTATCG	160	0.0067866463	18.616377	3
ATGGGGA	2990	0.0	18.328182	5
GAGTACT	2245	0.0	17.757393	12-13
>>END_MODULE