FastQCFastQC Report
Mon 15 Aug 2016
SRR2920942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052765
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.24912739646281967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42140.20528409243142787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.1459982024245347No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20590.10030373666737302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72400.039.0612341
GGTATCA49050.035.8830341
TCAACGC85450.032.0579454
ATCAACG86100.031.954263
TATCAAC87050.031.7423552
CAACGCA86300.031.6739655
AACGCAG89000.030.8957276
ACGCAGA99400.027.4149827
CGCAGAG102100.026.7469738
GTACATG74900.025.7292351
CATGGGG42350.024.4672854
TACATGG76600.023.6338652
GAGTACT60950.023.47741112-13
ACATGGG78100.023.40873
GCAGAGT113450.023.3369039
AGTACTT67100.022.7443612-13
GTACTTT67450.021.25905414-15
AGAGTAC104600.020.56195310-11
GTTCGCG1754.7857786E-420.399627
CAGAGTA108750.019.31226310-11
Produced by FastQC (version 0.11.5)