FastQCFastQC Report
Mon 15 Aug 2016
SRR2920941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102697
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67480.3209211788479272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25410.12084480074875266No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23340.11100030104194757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA2351.0913936E-1130.3884687
CATGGGG63900.028.1470224
GTACATG117650.027.9570161
GTACCGT3650.027.7212946
GTATCAA82850.027.5454541
TACATGG116550.027.186072
ACCGTAT3250.025.6335688
ACATGGG122850.025.1616553
AACGCAG87450.024.7061026
TCAACGC91900.023.5891464
TATCAAC95900.023.4136852
CAACGCA92450.023.3843925
GGTATCA62950.022.3388061
ATCAACG98650.021.9157623
GTACTAG2755.4371412E-821.6676081
CGCAGAG98400.021.5287748
GTATATA7750.021.5278191
GTATAGG2505.209695E-721.4509331
ACGCAGA99450.021.4226727
CCGTATG3358.894858E-1021.314249
Produced by FastQC (version 0.11.5)