FastQCFastQC Report
Mon 15 Aug 2016
SRR2920941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102697
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98260.46730460927085543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82420.3919727854274772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60900.2896280348523825No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT27030.12854919182364363No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25710.12227153983669545No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22830.10857484459244485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74450.044.8006061
GTATCAA114050.039.6561621
TATCAAC141900.031.8138522
TCAACGC142450.031.7298054
ATCAACG144100.031.4078143
CAACGCA144850.031.245195
AACGCAG147100.030.7908956
ACGCAGA165950.027.2504087
CGCAGAG168950.026.6577018
CATGGGG61550.026.0262954
GTACATG111150.025.6062341
GAGTACT106950.025.31498112-13
TACATGG110200.025.1845262
TATAGCG1901.160317E-625.0740495
GCAGAGT177050.024.6333839
AGTACTT108700.024.00419212-13
ACATGGG114450.023.7266883
GTACTTT117050.023.18144214-15
ATTATAC8300.022.2418883
AGAGTAC166050.022.05569310-11
Produced by FastQC (version 0.11.5)