FastQCFastQC Report
Mon 15 Aug 2016
SRR2920940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671282
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG174450.026.882841
GTATCAA128150.026.737491
TACATGG170850.026.0429782
CATGGGG98050.025.9990794
ACATGGG183750.023.5012553
AACGCAG142300.022.8388826
TATCAAC148150.022.4747682
TCAACGC144550.022.4564024
CAACGCA145250.022.307175
GGTATCA100750.022.2389451
ATCAACG147700.022.220383
ACGCAGA157400.020.3420547
CGCAGAG160150.019.879998
ATGGGGA59350.017.9636295
GTGTAGC34450.017.8163761
GTATAGA17750.017.792991
GCAGAGT178100.017.1076649
AGAGTAC152100.016.4308710-11
TATACTG24000.015.8829265
GAGTACT60700.015.87991712-13
Produced by FastQC (version 0.11.5)