FastQCFastQC Report
Mon 15 Aug 2016
SRR2920940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671282
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96600.206795479271001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83660.17909430430447143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.11305247681471595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159250.039.2264751
GGTATCA109700.038.3981321
ATCAACG192700.032.075293
TCAACGC193900.031.7539464
CAACGCA194450.031.6641275
TATCAAC198100.031.3819732
AACGCAG201400.030.8207046
ACGCAGA224700.027.3002327
CGCAGAG229800.026.6664628
GTACATG166950.025.1592861
TACATGG164200.024.7329272
CATGGGG95100.024.1691724
GCAGAGT256950.023.3396729
GAGTACT145650.023.26401712-13
ACATGGG168150.023.2306793
AGTACTT154100.021.67946412-13
CTATACG3901.2732926E-1121.3756054
AGAGTAC232100.021.05836110-11
GTACTTT163000.020.65999814-15
CAGAGTA244200.020.50220710-11
Produced by FastQC (version 0.11.5)