FastQCFastQC Report
Mon 15 Aug 2016
SRR2920939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6384973
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129450.029.230491
GTACATG217550.027.1443211
TCAACGC135300.026.6328414
CATGGGG126650.026.4766564
AACGCAG139500.026.0703956
CAACGCA138850.025.9948125
TACATGG214200.025.888522
ATCAACG139500.025.8747043
ACATGGG207850.025.8483873
GGTATCA98550.025.6978451
TATCAAC151650.024.115872
ACGCAGA162000.022.2631767
CGCAGAG167650.021.333718
GTACCGT6850.018.247756
GCAGAGT188000.018.106049
ATGGGGA87950.017.2688835
GTATATA41700.017.1478481
GTATTAG19250.016.715811
ATGGGGG76850.016.275545
GTATAAT36600.016.1182751
Produced by FastQC (version 0.11.5)