FastQCFastQC Report
Mon 15 Aug 2016
SRR2920939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6384973
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79660.12476168654119602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68350.10704822087736314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110050.040.0140041
GTATCAA161700.037.4174041
ATCAACG190850.031.201663
TCAACGC189900.031.2009624
CAACGCA192250.030.7578625
AACGCAG199750.029.6759176
TATCAAC203800.029.4240172
GTACATG221050.027.1282161
TACATGG214400.026.3583432
CATGGGG130650.025.8430354
ACATGGG214000.025.7115383
ACGCAGA232350.025.42947
CGCAGAG243250.024.2127368
GAGTACT137600.021.4052212-13
GCAGAGT265350.021.256999
AGAGTAC241800.019.7723110-11
AGTACTT156000.019.73870712-13
GTACTTT150600.019.47847414-15
CAGAGTA254200.019.05358510-11
ATGGGGA89900.018.613155
Produced by FastQC (version 0.11.5)