FastQCFastQC Report
Mon 15 Aug 2016
SRR2920938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617153
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94000.034.226561
GTACATG161250.030.0022371
GGTATCA66500.029.028241
TCAACGC107500.028.5873324
CAACGCA108650.028.3395655
ATCAACG110600.027.8408133
AACGCAG110850.027.8148566
TACATGG167600.027.753872
ACATGGG163600.027.7043443
CATGGGG106250.027.4662594
TATCAAC115750.027.0137462
ACGCAGA126800.024.0325837
CGCAGAG126850.023.8807988
ATGGGGA60400.019.3264265
AGAGTAC127450.019.16713510-11
GCAGAGT158050.018.5264459
ATGGGGG57950.017.8824985
GTATAAT25100.017.3279191
CAGAGTA141650.016.65752410-11
GAGTACT55300.016.409612-13
Produced by FastQC (version 0.11.5)