##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920938_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4617153
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.29172240989198	31.0	30.0	34.0	23.0	34.0
2	29.465995820368093	31.0	30.0	34.0	23.0	34.0
3	29.561190413226505	31.0	30.0	34.0	23.0	34.0
4	32.957944646841895	37.0	35.0	37.0	26.0	37.0
5	32.99155908413691	37.0	35.0	37.0	26.0	37.0
6	33.011760927134105	37.0	35.0	37.0	26.0	37.0
7	32.913476118291946	37.0	35.0	37.0	25.0	37.0
8	32.86360361027672	37.0	35.0	37.0	25.0	37.0
9	34.22585822908619	39.0	35.0	39.0	25.0	39.0
10-11	34.21603215228085	39.0	35.0	39.0	25.0	39.0
12-13	34.14652481734957	39.0	35.0	39.0	24.0	39.0
14-15	35.25365923546393	40.0	36.0	41.0	24.5	41.0
16-17	35.23403025630729	40.0	35.5	41.0	24.0	41.0
18-19	35.213700953812875	40.0	35.0	41.0	24.0	41.0
20-21	35.24213449283573	40.0	35.0	41.0	24.0	41.0
22-23	35.16021149829776	40.0	35.0	41.0	24.0	41.0
24-25	35.16446953349824	40.0	35.0	41.0	24.0	41.0
26-27	35.10171581925052	40.0	35.0	41.0	24.0	41.0
28-29	35.00101599405521	40.0	35.0	41.0	24.0	41.0
30-31	34.854317368300336	39.0	34.0	41.0	23.0	41.0
32-33	34.7220528537824	39.0	34.0	41.0	22.5	41.0
34-35	34.652462892176196	39.0	34.0	41.0	22.0	41.0
36-37	34.511245241385765	39.0	34.0	41.0	20.5	41.0
38-39	34.37097200374343	39.0	34.0	41.0	19.0	41.0
40-41	34.23928695886838	39.0	33.5	41.0	18.0	41.0
42-43	34.04561079089214	39.0	33.0	41.0	16.0	41.0
44-45	33.88492616553967	39.0	33.0	41.0	15.0	41.0
46-47	33.685416749239195	38.0	33.0	40.5	13.5	41.0
48-49	33.56062274739433	38.0	33.0	40.0	10.5	41.0
50-51	33.36702920609302	38.0	33.0	40.0	9.0	41.0
52-53	33.17584375046701	38.0	32.5	40.0	8.0	41.0
54-55	32.94232593115281	38.0	32.0	40.0	7.0	41.0
56-57	32.68127339509867	38.0	32.0	40.0	4.0	41.0
58-59	32.42433042613057	37.0	31.0	40.0	2.0	41.0
60-61	32.16244761652906	37.0	31.0	40.0	2.0	41.0
62-63	31.857760290811242	36.0	31.0	40.0	2.0	41.0
64-65	31.578411848167043	36.0	31.0	39.0	2.0	41.0
66-67	31.23779101537246	35.0	30.0	39.0	2.0	40.5
68-69	30.917138656657038	35.0	30.0	39.0	2.0	40.0
70-71	30.579081308330046	35.0	30.0	38.0	2.0	40.0
72-73	30.638487505179057	35.0	30.0	37.5	2.0	40.0
74-75	30.640930135951745	35.0	31.0	37.0	2.0	39.5
76-77	30.378283543993454	35.0	30.0	37.0	2.0	39.0
78-79	30.08840750999588	35.0	30.0	36.0	2.0	39.0
80-81	29.818051188687054	35.0	30.0	36.0	2.0	37.5
82-83	29.57526434579924	35.0	30.0	35.5	2.0	37.0
84-85	29.341851352987433	35.0	30.0	35.0	2.0	37.0
86-87	29.14384676011386	35.0	30.0	35.0	2.0	36.0
88-89	28.951695341263328	34.0	29.5	35.0	2.0	36.0
90-91	28.784384771308208	34.0	29.0	35.0	2.0	36.0
92-93	28.629842675778775	34.0	29.0	35.0	2.0	35.5
94-95	28.527356035201777	34.0	29.0	35.0	2.0	35.0
96-97	28.44349916496161	34.0	29.0	35.0	2.0	35.0
98-99	28.324532780265244	34.0	29.0	35.0	2.0	35.0
100-101	28.219124425809582	34.0	29.0	35.0	2.0	35.0
102-103	26.892367439415587	32.5	26.0	34.5	2.0	35.0
104-105	27.915267698514647	34.0	28.5	35.0	2.0	35.0
106-107	28.086529946051172	34.0	29.0	35.0	2.0	35.0
108-109	28.040609332201036	34.0	29.0	35.0	2.0	35.0
110-111	27.961091824334176	34.0	29.0	35.0	2.0	35.0
112-113	27.86631177264431	34.0	29.0	35.0	2.0	35.0
114-115	27.781377290291225	34.0	29.0	35.0	2.0	35.0
116-117	27.596414392158977	34.0	28.0	35.0	2.0	35.0
118-119	27.430498512828144	34.0	27.0	35.0	2.0	35.0
120-121	27.288897508919455	34.0	27.0	35.0	2.0	35.0
122-123	27.03938455147577	34.0	27.0	35.0	2.0	35.0
124-125	25.84494795818982	33.0	22.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	214790.0
3	30611.0
4	19227.0
5	17206.0
6	20616.0
7	22226.0
8	22679.0
9	24552.0
10	26653.0
11	28417.0
12	29448.0
13	28664.0
14	28959.0
15	25668.0
16	21287.0
17	19293.0
18	18350.0
19	18031.0
20	18338.0
21	19568.0
22	23231.0
23	30192.0
24	41293.0
25	53657.0
26	60064.0
27	65709.0
28	77033.0
29	89972.0
30	107063.0
31	129089.0
32	160705.0
33	204519.0
34	274236.0
35	399956.0
36	703543.0
37	1123091.0
38	418449.0
39	768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.955071799128184	18.600805351190303	12.313972422296974	25.130150427384546
2	15.738616214973492	20.37947215284729	39.32976403364844	24.552147598530784
3	19.004605971513744	24.773752882935515	29.229316029175713	26.99232511637503
4	12.545044786148013	16.48658150658177	36.90851844485716	34.05985526241306
5	13.434862093150443	38.07868670515015	34.64109769248809	13.845353509211325
6	32.4007984731272	36.742273870558805	17.357706117746382	13.49922153856761
7	27.624006690037856	32.64285765392063	21.699650342751696	18.033485313289816
8	25.428839502618583	36.3357987928368	20.037166162489665	18.19819554205495
9	25.802312129705427	16.402835398426554	19.46791185081378	38.32694062105424
10-11	24.65603778067577	26.730346192798333	28.09083680745833	20.52277921906757
12-13	25.12184694313809	24.227094329848587	27.905201436381283	22.745857290632042
14-15	22.475655566279695	25.667487341458006	25.170433252566333	26.686423839695966
16-17	22.687503765962806	28.331527838647297	26.064504928155184	22.916463467234717
18-19	22.757948346934135	27.570705495510122	27.354778176266482	22.31656798128926
20-21	23.608351597146783	26.871542856523632	27.041858222237874	22.478247324091708
22-23	23.19706747124114	26.679282839386513	26.794692105609606	23.328957583762737
24-25	22.93758147306553	26.750981469381298	27.10991265112136	23.20152440643181
26-27	22.832976504802772	26.928391254572702	27.043014924376774	23.19561731624776
28-29	22.83758082261758	27.169739227416674	26.98604985934776	23.006630090617993
30-31	23.128963106168253	26.971308891173862	26.872190940161993	23.027537062495888
32-33	22.80621541008418	27.034766749812327	26.85107830535435	23.30793953474914
34-35	23.035995829567092	27.091634545497694	26.942455199869002	22.929914425066205
36-37	23.18444526709298	26.83895181090566	26.887273983576314	23.089328938425048
38-39	22.99238338349598	27.041914472741713	26.866664889463372	23.099037254298935
40-41	23.14243678655192	26.919746593514677	26.92459078502562	23.013225834907786
42-43	23.03009022950763	26.99992847528821	26.852836821788596	23.117144473415564
44-45	22.998463730571913	27.042925710639746	26.849637724544085	23.108972834244256
46-47	23.010653136342636	27.08920105378408	26.72467637056513	23.17546943930816
48-49	22.871062099286217	27.1154263726824	26.7388263888919	23.274685139139486
50-51	22.85729765458653	27.23636718263235	26.72789627952084	23.178438883260284
52-53	23.094433311599815	27.113570221821675	26.61093971478848	23.181056751790035
54-55	22.961637412857865	27.048310233050156	26.625621971684097	23.364430382407882
56-57	22.88532661812532	27.218157242802864	26.52685969422006	23.369656444851756
58-59	22.952374977503272	26.984926730276204	26.670118783818907	23.392579508401614
60-61	22.96154307533813	26.983337648020967	26.819133893191598	23.235985383449307
62-63	22.859847466942213	26.987248891716785	26.864371186890274	23.28853245445073
64-65	22.832970320652414	26.873907142576975	26.763605607652025	23.52951692911859
66-67	22.897771058697273	27.097840493690605	26.550465630545155	23.453922817066967
68-69	23.115224275481356	26.857772337609088	26.635016067665372	23.39198731924418
70-71	22.906546829738392	26.72473443994822	26.74149684483214	23.627221885481244
72-73	23.007313388752042	26.76018980268868	26.775716316187857	23.456780492371422
74-75	22.951122703364295	26.851004295952652	26.712941774065484	23.48493122661757
76-77	22.968296554631983	26.68607651916067	26.838228003566854	23.50739892264049
78-79	23.056035450486256	26.738186421915916	26.765860443183087	23.439917684414738
80-81	23.035772738812422	26.684549406214813	26.71125866032666	23.5684191946461
82-83	23.065529579695145	26.672984699430934	26.79126902195791	23.470216698916012
84-85	23.087483164936646	26.736134024712772	26.632606986157132	23.543775824193446
86-87	23.015855558829053	26.601649170555042	26.78667457362404	23.595820696991865
88-89	23.105869921697828	26.51130909800915	26.74451664828643	23.63830433200659
90-91	23.03723144373848	26.60466825783828	26.762813827394755	23.59528647102848
92-93	23.145040401112755	26.535981775481954	26.764232487207895	23.554745336197392
94-95	23.01910410737821	26.419219770323345	26.87871828899461	23.682957833303842
96-97	23.090282085266445	26.32440807837545	26.864738252821564	23.720571583536547
98-99	23.03968810399163	26.48488695730748	26.715543433874593	23.7598815048263
100-101	22.961499612295135	26.430962477731484	26.880271082460926	23.72726682751245
102-103	22.820291112132157	26.507387876889627	26.904313660316166	23.768007350662053
104-105	22.7953464159635	26.62115715569529	26.74839022112487	23.835106207216338
106-107	22.688204093710386	26.678342488386754	26.7235678780115	23.909885539891363
108-109	22.752979428098897	26.62649599839121	26.73816225043579	23.882362323074112
110-111	22.72547596086123	26.829174206551304	26.657644088549848	23.78770574403762
112-113	22.770052023821016	26.890055616976554	26.607988572985214	23.731903786217217
114-115	22.69168674671379	26.961566047543467	26.567774263175053	23.778972942567687
116-117	22.720623620867016	26.934492219530824	26.561352189940994	23.78353196966116
118-119	22.846822981076723	26.823421004241833	26.474036203867612	23.85571981081383
120-121	22.764072064957546	26.910745790782077	26.569017390797423	23.756164753462958
122-123	22.863514192203024	26.90086018441114	26.489963251049563	23.74566237233627
124-125	22.972509626333956	26.89922594868643	26.350829427387556	23.77743499759206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2309.0
1	1709.0
2	884.5
3	608.0
4	512.0
5	438.5
6	422.5
7	460.5
8	550.0
9	643.5
10	684.0
11	739.0
12	824.5
13	942.0
14	1109.0
15	1271.0
16	1509.5
17	1774.5
18	2046.0
19	2503.5
20	3183.5
21	4234.0
22	5728.0
23	7663.5
24	10449.0
25	14173.0
26	19589.0
27	26648.0
28	32863.0
29	39602.0
30	47710.0
31	56645.0
32	67007.0
33	76278.5
34	83790.0
35	90726.5
36	98590.0
37	106874.0
38	114663.5
39	121372.0
40	129559.0
41	138351.0
42	145077.5
43	152967.0
44	160075.5
45	166415.0
46	172417.0
47	176300.0
48	181895.5
49	186421.5
50	187925.0
51	186712.5
52	180557.0
53	175119.5
54	168466.0
55	156628.5
56	142086.5
57	127995.0
58	116498.5
59	103697.5
60	89131.0
61	74660.0
62	60509.0
63	46287.5
64	33837.0
65	25282.5
66	19026.5
67	14538.0
68	11276.0
69	8675.0
70	6929.5
71	5405.5
72	4136.5
73	3534.5
74	2827.0
75	2177.5
76	1581.5
77	1007.5
78	671.0
79	476.0
80	341.5
81	242.0
82	194.0
83	146.0
84	105.5
85	79.5
86	54.0
87	42.0
88	34.0
89	26.5
90	22.0
91	19.5
92	17.0
93	17.0
94	17.0
95	12.5
96	13.0
97	14.0
98	11.5
99	9.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16215620318408336
2	0.1416024117026228
3	0.1668127523605997
4	0.13999969245117067
5	0.16365063059422114
6	0.10504308607490373
7	0.10660248858983014
8	0.0943221937847847
9	0.09982341932355285
10-11	0.08665513141972986
12-13	0.07527365889759338
14-15	0.07515453787214763
16-17	0.07491629582125608
18-19	0.07216568305187201
20-21	0.06639372790981801
22-23	0.05859671533518599
24-25	0.06276595122578783
26-27	0.07275045899496942
28-29	0.06743332958643562
30-31	0.06396799066437694
32-33	0.07282626328388944
34-35	0.0702489174606083
36-37	0.07210070794708341
38-39	0.06848376044718466
40-41	0.07320528472848961
42-43	0.07283709246802088
44-45	0.06613382749066363
46-47	0.06335072716888525
48-49	0.055770298276881874
50-51	0.06124986544738716
52-53	0.06342653145780527
54-55	0.06488847131554878
56-57	0.06819137247563596
58-59	0.05617097808974491
60-61	0.06645870301460662
62-63	0.0608383564503927
64-65	0.05479567170505287
66-67	0.059885388246826564
68-69	0.06317746022278231
70-71	0.057611259579225554
72-73	0.053409536136229396
74-75	0.05581361501340762
76-77	0.06500759234099454
78-79	0.05884578657020896
80-81	0.057340529975939726
82-83	0.06167220362851307
84-85	0.06279843877818214
86-87	0.05742716344899119
88-89	0.056181807273876345
90-91	0.059203149646546264
92-93	0.05394016615866964
94-95	0.062451904885976274
96-97	0.04958683413783342
98-99	0.04941356719173049
100-101	0.04707446341934088
102-103	0.04458375106911121
104-105	0.04332756570986493
106-107	0.05389684942214391
108-109	0.05434084597153268
110-111	0.0607084062408155
112-113	0.06117406115846713
114-115	0.06307999756559941
116-117	0.06643704464634376
118-119	0.05970129211659219
120-121	0.06098996502823277
122-123	0.05924646638307199
124-125	0.07032472174952833
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4617153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.466027324456284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.985195275115	35.11355609110704
2	13.577298732226279	13.432300601612216
3	5.799808082643027	8.606803952778671
4	3.0648303388253595	6.064199251406314
5	1.7607329981166346	4.354823329795464
6	1.1206246598298326	3.3259710026161216
7	0.7425279918022683	2.571093695216526
8	0.5572087475566522	2.2050322505650737
9	0.41147658521412556	1.831870080681831
>10	1.8571218772272629	15.738127574773117
>50	0.08648034527944498	2.947632642880408
>100	0.03485080628577554	2.9468537687026157
>500	0.0012729344808372605	0.4205978706118019
>1k	5.706253976655793E-4	0.4411378872526908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.165836826286675E-5	0.0
5	0.0	0.0	0.0	2.165836826286675E-5	2.165836826286675E-5
6	0.0	0.0	0.0	2.165836826286675E-5	2.165836826286675E-5
7	0.0	0.0	0.0	2.165836826286675E-5	2.165836826286675E-5
8	2.165836826286675E-5	0.0	0.0	2.165836826286675E-5	2.165836826286675E-5
9	2.165836826286675E-5	0.0	0.0	2.165836826286675E-5	2.165836826286675E-5
10-11	2.165836826286675E-5	0.0	0.0	5.4145920657166874E-5	6.497510478860025E-5
12-13	2.165836826286675E-5	0.0	0.0	7.580428892003363E-5	1.5160857784006723E-4
14-15	2.165836826286675E-5	0.0	0.0	1.0829184131433375E-4	2.1658368262866747E-4
16-17	2.165836826286675E-5	0.0	0.0	1.0829184131433375E-4	2.59900419154401E-4
18-19	2.165836826286675E-5	0.0	0.0	1.0829184131433375E-4	2.923879715487011E-4
20-21	2.165836826286675E-5	0.0	0.0	2.165836826286675E-4	3.140463398115679E-4
22-23	6.497510478860025E-5	0.0	0.0	4.0067981286303483E-4	3.2487552394300126E-4
24-25	6.497510478860025E-5	0.0	0.0	8.12188809857503E-4	3.2487552394300126E-4
26-27	6.497510478860025E-5	0.0	0.0	0.0016352068038464397	3.46533892205868E-4
28-29	6.497510478860025E-5	0.0	0.0	0.004407477941493383	4.33167365257335E-4
30-31	6.497510478860025E-5	0.0	0.0	0.015247491257058191	4.6565491765163515E-4
32-33	6.497510478860025E-5	0.0	0.0	0.04070690315005805	4.981424700459353E-4
34-35	6.497510478860025E-5	0.0	0.0	0.07691969488557127	5.414592065716687E-4
36-37	6.497510478860025E-5	0.0	0.0	0.1383319980949299	5.414592065716687E-4
38-39	6.497510478860025E-5	0.0	0.0	0.23255672922253173	5.847759430974022E-4
40-41	6.497510478860025E-5	0.0	0.0	0.33579134154748613	5.847759430974022E-4
42-43	6.497510478860025E-5	0.0	0.0	0.4336763369115123	6.172634954917024E-4
44-45	6.497510478860025E-5	0.0	0.0	0.5526349245953079	6.280926796231358E-4
46-47	6.497510478860025E-5	0.0	0.0	0.6963923439400861	6.280926796231358E-4
48-49	1.73266946102934E-4	0.0	0.0	0.8445897287787518	6.280926796231358E-4
50-51	1.73266946102934E-4	0.0	0.0	0.9996744747250091	6.280926796231358E-4
52-53	1.73266946102934E-4	0.0	0.0	1.1568384240245018	7.472137050689028E-4
54-55	1.73266946102934E-4	0.0	0.0	1.3299754199178584	8.121888098575031E-4
56-57	2.165836826286675E-4	0.0	0.0	1.5304236181906903	8.446763622518032E-4
58-59	2.165836826286675E-4	0.0	0.0	1.7499744972713704	8.6633473051467E-4
60-61	2.165836826286675E-4	0.0	0.0	1.9771707803488425	8.6633473051467E-4
62-63	2.165836826286675E-4	0.0	0.0	2.205049302026595	8.879930987775367E-4
64-65	2.165836826286675E-4	0.0	0.0	2.449778034212858	8.879930987775367E-4
66-67	2.165836826286675E-4	0.0	0.0	2.7271242690029984	9.204806511718368E-4
68-69	2.3824205089153423E-4	0.0	0.0	3.0341857850497913	9.529682035661369E-4
70-71	2.3824205089153423E-4	0.0	0.0	3.372392034658587	9.854557559604371E-4
72-73	2.3824205089153423E-4	0.0	0.0	3.7077610380249473	0.001039601676617604
74-75	2.3824205089153423E-4	0.0	0.0	4.052497285664998	0.0010829184131433375
76-77	2.3824205089153423E-4	0.0	0.0	4.434128563640841	0.0010937475972747708
78-79	2.59900419154401E-4	0.0	0.0	4.8532504770797065	0.0011045767814062042
80-81	2.59900419154401E-4	0.0	0.0	5.299510325951944	0.0011045767814062042
82-83	2.7072960328583436E-4	0.0	0.0	5.768446486395405	0.0011695518861948044
84-85	2.8155878741726773E-4	0.0	0.0	6.2346861799901365	0.0011695518861948044
86-87	2.8155878741726773E-4	0.0	0.0	6.726471918950921	0.001212868622720538
88-89	2.8155878741726773E-4	0.0	0.0	7.257047795470498	0.0012561853592462716
90-91	2.923879715487011E-4	0.0	0.0	7.8352829113525155	0.0012561853592462716
92-93	3.0321715568013447E-4	0.0	0.0	8.425863297144366	0.0013428188322977385
94-95	3.0321715568013447E-4	0.0	0.0	9.017873135241565	0.001386135568823472
96-97	3.357047080744346E-4	2.165836826286675E-5	0.0	9.628769070463985	0.0014511106736120722
98-99	3.46533892205868E-4	2.165836826286675E-5	0.0	10.279462257369422	0.001472769041874939
100-101	3.46533892205868E-4	2.165836826286675E-5	0.0	10.984539606982917	0.001472769041874939
102-103	3.46533892205868E-4	2.165836826286675E-5	0.0	11.715704461169036	0.0014944274101378057
104-105	3.6819226046873473E-4	2.165836826286675E-5	0.0	12.43791358007846	0.0015160857784006724
106-107	3.6819226046873473E-4	3.2487552394300124E-5	0.0	13.159624556517837	0.0015269149625321058
108-109	3.6819226046873473E-4	4.33167365257335E-5	0.0	13.915631559101463	0.0015810608831892726
110-111	3.6819226046873473E-4	4.33167365257335E-5	0.0	14.703931188765026	0.0016027192514521396
112-113	3.6819226046873473E-4	4.33167365257335E-5	0.0	15.530468667596677	0.0016027192514521396
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9400	0.0	34.22656	1
GTACATG	16125	0.0	30.002237	1
GGTATCA	6650	0.0	29.02824	1
TCAACGC	10750	0.0	28.587332	4
CAACGCA	10865	0.0	28.339565	5
ATCAACG	11060	0.0	27.840813	3
AACGCAG	11085	0.0	27.814856	6
TACATGG	16760	0.0	27.75387	2
ACATGGG	16360	0.0	27.704344	3
CATGGGG	10625	0.0	27.466259	4
TATCAAC	11575	0.0	27.013746	2
ACGCAGA	12680	0.0	24.032583	7
CGCAGAG	12685	0.0	23.880798	8
ATGGGGA	6040	0.0	19.326426	5
AGAGTAC	12745	0.0	19.167135	10-11
GCAGAGT	15805	0.0	18.526445	9
ATGGGGG	5795	0.0	17.882498	5
GTATAAT	2510	0.0	17.327919	1
CAGAGTA	14165	0.0	16.657524	10-11
GAGTACT	5530	0.0	16.4096	12-13
>>END_MODULE