FastQCFastQC Report
Mon 15 Aug 2016
SRR2920938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617153
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81400.17629911765973535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69730.15102380189696984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.046.235881
GTATCAA132350.041.1002731
TCAACGC162100.032.762584
CAACGCA163500.032.4816935
ATCAACG164650.032.3274963
TATCAAC170200.031.483912
AACGCAG170150.031.4068326
ACGCAGA194750.027.2833237
CGCAGAG195500.027.1455758
GTACATG158800.026.967941
TACATGG162700.025.3967192
ACATGGG159200.025.281333
GAGTACT130700.023.60535612-13
CATGGGG101650.023.194454
GCAGAGT226250.022.9322419
AGAGTAC200100.022.49532110-11
AGTACTT140400.021.72021912-13
CAGAGTA213950.021.1364310-11
GTACTTT149850.020.64828714-15
TACTTTT178250.017.00795414-15
Produced by FastQC (version 0.11.5)