FastQCFastQC Report
Mon 15 Aug 2016
SRR2920937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774649
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG146800.030.3984261
GTATCAA91500.030.3431151
TACATGG146900.029.472162
CATGGGG101550.029.203664
ACATGGG154400.027.5005633
TATCAAC105550.025.840842
GGTATCA68750.025.8249171
TCAACGC102700.025.629474
AACGCAG106200.024.603536
CAACGCA106800.024.4782925
ATCAACG108250.024.2610763
ACGCAGA116400.022.2914877
CGCAGAG119300.021.4488878
ATGGGGG51000.020.3173435
ATGGGGA56300.018.9335675
GCAGAGT135800.017.8346929
ATGGGGC42700.017.711845
GTATAAT14550.017.6076241
GTATAGA11200.017.0226551
GTATAAG13100.016.827741
Produced by FastQC (version 0.11.5)