FastQCFastQC Report
Mon 15 Aug 2016
SRR2920937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774649
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.20817829684296477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64710.1714331584208227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.11587832405079254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133400.040.3434831
GGTATCA91200.037.4217341
TATCAAC163650.032.6396832
TCAACGC163400.032.542124
CAACGCA165700.032.1263545
ATCAACG165800.032.0710643
AACGCAG170750.031.333126
ACGCAGA191150.027.8598377
CATGGGG99700.027.712094
GTACATG156400.027.3530081
CGCAGAG199350.026.5916468
TACATGG157750.026.310732
ACATGGG158150.025.7156753
GAGTACT117000.023.72379112-13
GCAGAGT218850.023.5160759
AGTACTT122650.023.35861412-13
TACCGTA3103.3833203E-1023.032947
ATGGGGA53350.022.4340695
AGAGTAC196350.021.89386410-11
GTACTTT127350.021.72562814-15
Produced by FastQC (version 0.11.5)