FastQCFastQC Report
Mon 15 Aug 2016
SRR2920936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5038559
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG197650.032.080411
TACATGG200550.031.008352
CATGGGG138450.030.7604224
ACATGGG199850.030.6397673
GTATCAA93700.027.2207031
TATACCG4550.026.18175
TCAACGC101650.024.5519434
CAACGCA103950.023.9514065
GGTATCA74000.023.354011
AACGCAG109000.022.9896686
ATCAACG111800.022.4303873
TATCAAC113950.021.8505692
ATGGGGG74200.020.7909855
ATGGGGA68250.020.5089975
GTATTAC11650.019.9495831
ACGCAGA125550.019.6721767
GTATATA12600.018.445451
GTATAGG11100.018.253711
CGCAGAG134150.018.2315968
ATGGGGC61000.018.06435