##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920936_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5038559 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.97202692277693 31.0 30.0 34.0 26.0 34.0 2 30.126588971172115 31.0 31.0 34.0 26.0 34.0 3 30.225944362267068 31.0 31.0 34.0 26.0 34.0 4 33.71217941478903 37.0 35.0 37.0 28.0 37.0 5 33.74783127477519 37.0 35.0 37.0 28.0 37.0 6 33.73564286932037 37.0 35.0 37.0 29.0 37.0 7 33.63012023874286 37.0 35.0 37.0 28.0 37.0 8 33.55699377540285 37.0 35.0 37.0 28.0 37.0 9 35.072784302019684 39.0 35.0 39.0 28.0 39.0 10-11 35.043327566472875 39.0 35.0 39.0 27.5 39.0 12-13 34.99020682302222 39.0 35.0 39.0 27.0 39.0 14-15 36.15483692857422 40.0 36.0 41.0 27.0 41.0 16-17 36.111235275800084 40.0 36.0 41.0 27.0 41.0 18-19 36.060815602238655 40.0 36.0 41.0 27.0 41.0 20-21 36.05904862878454 40.0 36.0 41.0 27.0 41.0 22-23 35.95145467186154 40.0 36.0 41.0 27.0 41.0 24-25 35.925440984217914 40.0 36.0 41.0 27.0 41.0 26-27 35.841112409321795 40.0 36.0 41.0 27.0 41.0 28-29 35.731360196437116 40.0 36.0 41.0 26.5 41.0 30-31 35.590148889791706 40.0 35.5 41.0 25.5 41.0 32-33 35.471555359379536 40.0 35.0 41.0 25.0 41.0 34-35 35.41977299462009 40.0 35.0 41.0 25.0 41.0 36-37 35.29742323152314 40.0 35.0 41.0 25.0 41.0 38-39 35.17290568593124 39.0 35.0 41.0 24.5 41.0 40-41 35.04376737475933 39.0 35.0 41.0 24.0 41.0 42-43 34.8684011639042 39.0 34.5 41.0 24.0 41.0 44-45 34.7202225874501 39.0 34.0 41.0 23.0 41.0 46-47 34.52755281817679 39.0 34.0 41.0 23.0 41.0 48-49 34.413217548906346 39.0 34.0 41.0 22.5 41.0 50-51 34.20181027948665 38.0 33.0 40.0 22.0 41.0 52-53 33.990974403594365 38.0 33.0 40.0 20.5 41.0 54-55 33.73273945983365 38.0 33.0 40.0 19.5 41.0 56-57 33.440879922215856 38.0 33.0 40.0 18.5 41.0 58-59 33.15403392120644 37.0 32.0 40.0 18.0 41.0 60-61 32.86124574506322 37.0 32.0 40.0 16.0 41.0 62-63 32.52390137735809 36.0 32.0 39.5 13.0 41.0 64-65 32.21353734272041 36.0 31.0 39.0 13.0 41.0 66-67 31.841767160015394 35.0 31.0 39.0 10.5 40.0 68-69 31.500351290914722 35.0 31.0 38.0 8.0 40.0 70-71 31.141699739945487 35.0 30.5 37.0 8.0 40.0 72-73 31.20732207363256 35.0 31.0 37.0 8.0 39.0 74-75 31.200249912723063 35.0 31.0 37.0 7.5 39.0 76-77 30.94609510377868 35.0 31.0 36.0 7.0 39.0 78-79 30.66825663845556 35.0 31.0 36.0 4.0 37.5 80-81 30.418672680026177 35.0 31.0 35.0 2.0 37.0 82-83 30.2038045004534 35.0 31.0 35.0 2.0 37.0 84-85 29.994926426385003 35.0 31.0 35.0 2.0 36.0 86-87 29.81681181067841 35.0 31.0 35.0 2.0 36.0 88-89 29.636736614575717 35.0 30.5 35.0 2.0 36.0 90-91 29.47438186195696 34.5 30.0 35.0 2.0 35.0 92-93 29.316613837408674 34.0 30.0 35.0 2.0 35.0 94-95 29.204684216261036 34.0 30.0 35.0 2.0 35.0 96-97 29.10391830283222 34.0 30.0 35.0 2.0 35.0 98-99 28.95869493638955 34.0 30.0 35.0 2.0 35.0 100-101 28.81380351009088 34.0 29.5 35.0 2.0 35.0 102-103 27.420894346974997 32.5 26.0 34.5 2.0 35.0 104-105 28.435715846534695 34.0 29.0 35.0 2.0 35.0 106-107 28.567144098143935 34.0 29.0 35.0 2.0 35.0 108-109 28.470289719739313 34.0 29.0 35.0 2.0 35.0 110-111 28.333741849604223 34.0 29.0 35.0 2.0 35.0 112-113 28.178995621565612 34.0 29.0 35.0 2.0 35.0 114-115 28.027919391238648 34.0 29.0 35.0 2.0 35.0 116-117 27.768469814484657 34.0 29.0 35.0 2.0 35.0 118-119 27.527350180875125 34.0 27.5 35.0 2.0 35.0 120-121 27.295060055861207 34.0 27.0 35.0 2.0 35.0 122-123 26.94908802298435 34.0 27.0 35.0 2.0 35.0 124-125 25.57100542039897 32.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 172675.0 3 27027.0 4 18379.0 5 16439.0 6 18316.0 7 20355.0 8 21610.0 9 23435.0 10 24649.0 11 25608.0 12 25512.0 13 24205.0 14 22856.0 15 21169.0 16 19448.0 17 18722.0 18 19001.0 19 20037.0 20 21572.0 21 24105.0 22 28898.0 23 36404.0 24 46020.0 25 56958.0 26 66411.0 27 76889.0 28 89607.0 29 104818.0 30 123916.0 31 151782.0 32 188244.0 33 240674.0 34 322791.0 35 473017.0 36 847814.0 37 1253861.0 38 345114.0 39 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.7941290551788 17.05620942684003 10.964666298340125 26.184995219641046 2 17.191738548372186 19.910611260357374 36.50348302021434 26.3941671710561 3 19.538979101260765 23.294846992438597 28.720387156550665 28.445786749749974 4 13.576302487234667 15.12452074722983 34.94487885034592 36.354297915189576 5 14.63555215526531 36.63801913299491 33.89348652665918 14.832942185080592 6 34.836134789383145 34.01789159420129 16.82244587598038 14.323527740435186 7 29.789689312402274 30.992741946700097 20.812731098364207 18.404837642533426 8 27.799210234019377 33.424281380304485 19.801940662061256 18.974567723614886 9 27.33114697296639 14.320865701751545 18.413486745446896 39.934500579835166 10-11 26.369004300643155 24.689460072075676 27.361234259456346 21.580301367824823 12-13 26.402231509917932 22.151630967831125 27.245940009748505 24.200197512502438 14-15 23.94391878484742 23.610497858133282 24.05105681115999 28.394526545859307 16-17 24.443646199462286 26.27152679348897 24.963284482594243 24.3215425244545 18-19 24.428526994936835 25.918001444348487 25.850233058102056 23.80323850261262 20-21 25.293058724156086 25.228372600809163 25.364566979332814 24.114001695701937 22-23 24.931739526493047 25.054706681036663 25.203897693392758 24.80965609907753 24-25 24.560396589382155 25.18568778158509 25.39748108069892 24.856434548333834 26-27 24.46115060731286 25.260476976055863 25.490158302153688 24.78821411447759 28-29 24.597455468514305 25.40596059648467 25.439733960035614 24.55684997496541 30-31 24.762954051540646 25.40277578484465 25.19604917626626 24.638220987348447 32-33 24.46309773586056 25.475642875990477 25.21777446832641 24.843484919822547 34-35 24.710485675405316 25.30857644446329 25.385142708691976 24.595795171439413 36-37 24.765272518052885 25.156426229335626 25.373061846618526 24.705239405992963 38-39 24.66620006407179 25.367255898090495 25.339073048397243 24.627470989440468 40-41 24.76125536660873 25.26656643601169 25.413615313898756 24.55856288348082 42-43 24.58770281174353 25.228268884612998 25.381654770032895 24.802373533610577 44-45 24.616882416600514 25.20339824268685 25.375739338833814 24.80398000187882 46-47 24.555676679626657 25.194355146059543 25.486853558747235 24.763114615566565 48-49 24.411869517639122 25.29834101474917 25.471397519897426 24.81839194771428 50-51 24.345846774565917 25.427282000808088 25.492292435543895 24.7345787890821 52-53 24.48935940131309 25.23637748066395 25.396112324398175 24.878150793624787 54-55 24.33348427253264 25.174592960672392 25.49495148099159 24.996971285803372 56-57 24.244935784685246 25.284422681837466 25.527325471465772 24.943316062011515 58-59 24.332881219951506 25.150974757775124 25.52002637217735 24.996117650096018 60-61 24.387540921550283 25.0824736750067 25.645968896493738 24.884016506949276 62-63 24.25528644372038 25.1002396377501 25.634119538058098 25.010354380471423 64-65 24.143707130993793 25.061995884714417 25.621510295710564 25.172786688581226 66-67 24.167954471726198 25.131904237945268 25.45909923518132 25.24104205514722 68-69 24.24933284954126 24.989528761498253 25.521034727174836 25.24010366178565 70-71 23.981025844402946 24.802903635223725 25.753011479936077 25.463059040437248 72-73 23.97240483917154 24.823154024295942 25.743475931432492 25.460965205100027 74-75 23.833569950652926 24.86853986559977 25.827410949771796 25.470479233975507 76-77 23.81410599200938 24.726503642827982 25.853239617912937 25.606150747249696 78-79 23.745762111030977 24.788712506914823 25.836300091442975 25.629225290611224 80-81 23.730133858886948 24.80143788482146 25.90206400537628 25.566364250915306 82-83 23.700464448240812 24.611179010577885 26.058404177611234 25.62995236357007 84-85 23.64874627355662 24.622911883398434 25.927923005753872 25.80041883729108 86-87 23.51400467520882 24.62750945934433 26.12782809720669 25.730657768240157 88-89 23.563941303953666 24.405774122574893 26.05938316763317 25.970901405838266 90-91 23.483383768298708 24.45549848695104 26.130402726715307 25.93071501803495 92-93 23.50041925796553 24.38045885537859 26.20857252219535 25.91054936446053 94-95 23.4618365948208 24.240041611248227 26.250060325585377 26.0480614683456 96-97 23.457486116871166 24.115127082593435 26.282891852930923 26.144494947604475 98-99 23.46661053601463 24.089422483106198 26.18247691064305 26.26149007023613 100-101 23.42273799778495 24.063127513466796 26.325588922552086 26.18854556619617 102-103 23.230771796874784 23.95980314793333 26.377394577434053 26.43203047775784 104-105 23.096168434822467 24.00305628502717 26.35454117550814 26.54623410464222 106-107 23.075254179394875 23.943339057613667 26.451914307573077 26.529492455418378 108-109 22.97998016902599 24.00571575299416 26.48309809619869 26.531205981781163 110-111 23.00680835623622 24.166442329758077 26.3164720355727 26.510277278433 112-113 22.930912360243212 24.21329909981102 26.32129019970426 26.534498340241502 114-115 22.917034709807233 24.304233372468794 26.26965573803213 26.509076179691842 116-117 22.711003934257374 24.261329945233015 26.365621877716368 26.662044242793243 118-119 22.76409855238597 24.318569223262156 26.2225557375392 26.69477648681267 120-121 22.787794436184257 24.396570103385258 26.20269659837438 26.6129388620561 122-123 22.72658621296746 24.542340271027545 26.075609715651556 26.655463800353445 124-125 22.775548760752933 24.618785454355503 25.889707502715385 26.715958282176178 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1268.0 1 1017.0 2 567.5 3 322.0 4 269.5 5 252.0 6 225.5 7 224.0 8 260.0 9 283.0 10 269.5 11 271.0 12 298.5 13 339.5 14 378.5 15 406.5 16 462.0 17 554.5 18 641.5 19 739.5 20 864.5 21 1039.5 22 1218.0 23 1462.0 24 1922.0 25 2504.5 26 3227.0 27 4383.0 28 6001.5 29 8505.5 30 12099.5 31 16719.0 32 22840.5 33 29281.5 34 36984.5 35 46252.0 36 57862.0 37 70987.0 38 84577.5 39 97440.5 40 111062.0 41 126723.5 42 140916.5 43 156739.5 44 173225.0 45 188365.5 46 205469.0 47 219418.5 48 229462.5 49 238519.5 50 246156.5 51 250361.0 52 247243.5 53 244538.0 54 240937.0 55 229545.5 56 212370.0 57 190081.0 58 169373.5 59 149948.0 60 126907.5 61 104230.0 62 83341.5 63 63114.0 64 45589.5 65 33509.5 66 25232.0 67 19103.0 68 14448.5 69 10668.0 70 7761.0 71 5737.5 72 4083.0 73 2764.0 74 2038.5 75 1509.0 76 1033.0 77 662.5 78 452.5 79 322.5 80 232.5 81 156.0 82 105.5 83 78.5 84 60.5 85 50.5 86 47.0 87 40.5 88 29.0 89 21.5 90 26.0 91 27.0 92 21.0 93 18.5 94 14.5 95 9.0 96 10.0 97 12.5 98 10.5 99 9.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17072341516691578 2 0.15329779804106689 3 0.17999193817121126 4 0.1498444297268326 5 0.177094284298348 6 0.11076579633184805 7 0.1097337552264447 8 0.09756757834928598 9 0.1038392127590448 10-11 0.09019443852895243 12-13 0.07821680762297316 14-15 0.07803818512396105 16-17 0.07746262373825533 18-19 0.07468405153140015 20-21 0.06898797850734704 22-23 0.06089042521879767 24-25 0.0640063954793424 26-27 0.07456496986539207 28-29 0.06998032572408103 30-31 0.0676086158760868 32-33 0.07582525083064423 34-35 0.07352300528782138 36-37 0.0761626488843338 38-39 0.0710917546068231 40-41 0.07618249582866847 42-43 0.07683744499171291 44-45 0.06918644795069384 46-47 0.06585216130246763 48-49 0.0579630009294324 50-51 0.0637781556194936 52-53 0.06627887060566325 54-55 0.06785670268027029 56-57 0.07190547932454497 58-59 0.05852863884297078 60-61 0.06881927948050226 62-63 0.06451249255987675 64-65 0.05839963370479536 66-67 0.06316290034511851 68-69 0.06624910018916123 70-71 0.06108889466214447 72-73 0.055988229968131756 74-75 0.05958052689270881 76-77 0.07002994308491774 78-79 0.06312320645644916 80-81 0.06166445604785019 82-83 0.06622925324482655 84-85 0.06806509559578443 86-87 0.06077134355278959 88-89 0.060691955775450876 90-91 0.06344075756580403 92-93 0.057089735378706485 94-95 0.06712236573988714 96-97 0.05319973428910925 98-99 0.053368433315954034 100-101 0.05083794791328235 102-103 0.049478432226356776 104-105 0.04722580404437063 106-107 0.059372133977194666 108-109 0.05958052689270881 110-111 0.06647734004901004 112-113 0.06625902366132856 114-115 0.06913683058985713 116-117 0.07308637251245842 118-119 0.06565369185912083 120-121 0.06675519726969556 122-123 0.06431402311652994 124-125 0.07598402638532167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 5038559.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.69111272477766 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81041581947319 31.89715578571152 2 13.80258174864471 12.613106371405685 3 5.917428863447374 8.11121727721881 4 3.134952437147711 5.7295786077013 5 1.8159264055895434 4.148584904884609 6 1.2060899719377016 3.306455571841767 7 0.8196523077366503 2.6215578191543605 8 0.5849364081177167 2.138111628810652 9 0.41487535930230673 1.7060505127752896 >10 2.2993328508968793 18.827606467368568 >50 0.13922806499733797 4.352054381751006 >100 0.05296728552849558 3.9662028796823923 >500 0.0014381557071773971 0.42769835361721975 >1k 1.7432147309122493E-4 0.15461943807664896 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9846944334679816E-5 0.0 12-13 0.0 0.0 0.0 1.9846944334679816E-5 9.923472167339908E-6 14-15 0.0 0.0 0.0 1.9846944334679816E-5 2.9770416502019724E-5 16-17 0.0 0.0 0.0 1.9846944334679816E-5 4.961736083669954E-5 18-19 0.0 0.0 0.0 2.9770416502019724E-5 5.954083300403945E-5 20-21 0.0 0.0 0.0 5.954083300403945E-5 5.954083300403945E-5 22-23 0.0 0.0 0.0 1.5877555467743853E-4 5.954083300403945E-5 24-25 0.0 0.0 0.0 3.3739805368955685E-4 5.954083300403945E-5 26-27 0.0 0.0 0.0 8.53418606391232E-4 5.954083300403945E-5 28-29 0.0 0.0 0.0 0.004435792058800939 7.938777733871926E-5 30-31 0.0 0.0 0.0 0.020005719889357253 7.938777733871926E-5 32-33 0.0 9.923472167339908E-6 0.0 0.05268371373640757 9.923472167339908E-5 34-35 0.0 1.9846944334679816E-5 0.0 0.10504987636346026 1.190816660080789E-4 36-37 0.0 1.9846944334679816E-5 0.0 0.19579010586161638 1.190816660080789E-4 38-39 0.0 1.9846944334679816E-5 0.0 0.34177430491535377 1.190816660080789E-4 40-41 0.0 1.9846944334679816E-5 0.0 0.5074268258047588 1.190816660080789E-4 42-43 0.0 2.9770416502019724E-5 0.0 0.6696458253242643 1.190816660080789E-4 44-45 0.0 3.969388866935963E-5 0.0 0.8696037894961635 1.190816660080789E-4 46-47 0.0 3.969388866935963E-5 0.0 1.1107143927460212 1.6869902684477843E-4 48-49 0.0 3.969388866935963E-5 0.0 1.3724955885204482 1.9846944334679816E-4 50-51 0.0 3.969388866935963E-5 0.0 1.6503329622616307 1.9846944334679816E-4 52-53 0.0 3.969388866935963E-5 0.0 1.9215613035393653 1.9846944334679816E-4 54-55 0.0 3.969388866935963E-5 0.0 2.229704961279604 1.9846944334679816E-4 56-57 0.0 3.969388866935963E-5 0.0 2.591276593168801 2.1831638768147799E-4 58-59 0.0 3.969388866935963E-5 0.0 2.989406296522478 2.1831638768147799E-4 60-61 0.0 3.969388866935963E-5 0.0 3.4068073828251295 2.1831638768147799E-4 62-63 0.0 3.969388866935963E-5 0.0 3.8251214285671757 2.1831638768147799E-4 64-65 0.0 3.969388866935963E-5 0.0 4.271975380262491 2.1831638768147799E-4 66-67 0.0 3.969388866935963E-5 0.0 4.790268408090488 2.1831638768147799E-4 68-69 0.0 3.969388866935963E-5 0.0 5.368757218085568 2.1831638768147799E-4 70-71 0.0 3.969388866935963E-5 0.0 5.985669712312588 2.1831638768147799E-4 72-73 0.0 3.969388866935963E-5 0.0 6.603515012923338 2.480868041834977E-4 74-75 0.0 3.969388866935963E-5 1.9846944334679816E-5 7.2233946253283925 2.580102763508376E-4 76-77 0.0 3.969388866935963E-5 1.9846944334679816E-5 7.907350097517961 2.580102763508376E-4 78-79 0.0 3.969388866935963E-5 1.9846944334679816E-5 8.675883322989767 2.580102763508376E-4 80-81 0.0 3.969388866935963E-5 1.9846944334679816E-5 9.48296923783169 2.580102763508376E-4 82-83 0.0 3.969388866935963E-5 1.9846944334679816E-5 10.320381283617003 2.580102763508376E-4 84-85 0.0 4.961736083669954E-5 1.9846944334679816E-5 11.143801233646366 2.580102763508376E-4 86-87 0.0 5.954083300403945E-5 1.9846944334679816E-5 12.011549333847237 2.679337485181775E-4 88-89 0.0 5.954083300403945E-5 1.9846944334679816E-5 12.958069956112451 2.7785722068551745E-4 90-91 0.0 5.954083300403945E-5 1.9846944334679816E-5 13.96225587514208 2.7785722068551745E-4 92-93 1.9846944334679816E-5 5.954083300403945E-5 1.9846944334679816E-5 14.981515548393896 2.9770416502019725E-4 94-95 1.9846944334679816E-5 5.954083300403945E-5 1.9846944334679816E-5 15.98403432409941 2.9770416502019725E-4 96-97 1.9846944334679816E-5 7.938777733871926E-5 1.9846944334679816E-5 17.011808336470803 3.0762763718753715E-4 98-99 1.9846944334679816E-5 1.0915819384073899E-4 1.9846944334679816E-5 18.118305253545707 3.1755110935487705E-4 100-101 1.9846944334679816E-5 1.190816660080789E-4 1.9846944334679816E-5 19.293522215379436 3.1755110935487705E-4 102-103 1.9846944334679816E-5 1.3892861034275872E-4 1.9846944334679816E-5 20.476916118279057 3.2747458152221695E-4 104-105 1.9846944334679816E-5 1.3892861034275872E-4 1.9846944334679816E-5 21.651815529003432 3.3739805368955685E-4 106-107 1.9846944334679816E-5 1.3892861034275872E-4 1.9846944334679816E-5 22.805518403178368 3.3739805368955685E-4 108-109 1.9846944334679816E-5 1.3892861034275872E-4 1.9846944334679816E-5 24.008064607360954 3.3739805368955685E-4 110-111 3.969388866935963E-5 1.3892861034275872E-4 1.9846944334679816E-5 25.25767784003323 3.572449980242367E-4 112-113 3.969388866935963E-5 1.3892861034275872E-4 1.9846944334679816E-5 26.53899656628016 3.572449980242367E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 19765 0.0 32.08041 1 TACATGG 20055 0.0 31.00835 2 CATGGGG 13845 0.0 30.760422 4 ACATGGG 19985 0.0 30.639767 3 GTATCAA 9370 0.0 27.220703 1 TATACCG 455 0.0 26.1817 5 TCAACGC 10165 0.0 24.551943 4 CAACGCA 10395 0.0 23.951406 5 GGTATCA 7400 0.0 23.35401 1 AACGCAG 10900 0.0 22.989668 6 ATCAACG 11180 0.0 22.430387 3 TATCAAC 11395 0.0 21.850569 2 ATGGGGG 7420 0.0 20.790985 5 ATGGGGA 6825 0.0 20.508997 5 GTATTAC 1165 0.0 19.949583 1 ACGCAGA 12555 0.0 19.672176 7 GTATATA 1260 0.0 18.44545 1 GTATAGG 1110 0.0 18.25371 1 CGCAGAG 13415 0.0 18.231596 8 ATGGGGC 6100 0.0 18.0643 5 >>END_MODULE