FastQCFastQC Report
Mon 15 Aug 2016
SRR2920936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5038559
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG198100.032.1107371
CATGGGG141350.030.9646594
TACATGG203600.030.741542
GTATCAA95200.030.2125641
ACATGGG199550.030.1699223
GGTATCA74650.029.4168071
TCAACGC111650.025.3343434
CAACGCA114800.024.6910655
TATCAAC119550.024.0103282
ATGGGGG71050.023.8866655
AACGCAG120850.023.5397326
ATCAACG120500.023.5231083
ATGGGGA74500.021.1818755
GTATATA11850.021.1499061
ACGCAGA142050.019.7714777
TATACCG5001.8189894E-1219.0556935
CGCAGAG154850.017.866228
GTATAAT15850.017.6948181
ACCGTCG4451.6043487E-917.380988
GAGTACA146150.017.1485731