##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5038559
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.186213955220133	31.0	31.0	34.0	28.0	34.0
2	31.277493823134748	33.0	31.0	34.0	28.0	34.0
3	31.38353942069548	33.0	31.0	34.0	28.0	34.0
4	34.94179526328857	37.0	35.0	37.0	32.0	37.0
5	34.64232075083372	37.0	35.0	37.0	32.0	37.0
6	34.40616136478704	37.0	35.0	37.0	30.0	37.0
7	34.304064912210016	37.0	35.0	37.0	30.0	37.0
8	34.28828579758618	37.0	35.0	37.0	30.0	37.0
9	35.86836295853636	39.0	37.0	39.0	30.0	39.0
10-11	35.82700668980952	39.0	36.0	39.0	30.0	39.0
12-13	35.71064941385027	39.0	35.0	39.0	30.0	39.0
14-15	36.94614988134504	40.0	37.0	41.0	30.0	41.0
16-17	36.92958512542971	40.0	37.0	41.0	30.0	41.0
18-19	36.931646528303034	40.0	37.0	41.0	30.0	41.0
20-21	36.915011911143644	40.0	37.0	41.0	30.0	41.0
22-23	36.87289282511131	40.0	37.0	41.0	30.0	41.0
24-25	36.85987273345415	40.0	37.0	41.0	30.0	41.0
26-27	36.74574387240479	40.0	37.0	41.0	30.0	41.0
28-29	36.659754604441474	40.0	36.5	41.0	30.0	41.0
30-31	36.55601720650686	40.0	36.0	41.0	30.0	41.0
32-33	36.45954299632098	40.0	36.0	41.0	29.5	41.0
34-35	36.37029793637427	40.0	36.0	41.0	29.0	41.0
36-37	36.27266009984204	40.0	36.0	41.0	29.0	41.0
38-39	36.172063480848394	40.0	36.0	41.0	28.0	41.0
40-41	36.05757410005519	40.0	35.0	41.0	28.0	41.0
42-43	35.9441451414978	40.0	35.0	41.0	28.0	41.0
44-45	35.793756905496195	40.0	35.0	41.0	27.0	41.0
46-47	35.659705780958404	39.5	35.0	41.0	27.0	41.0
48-49	35.47599244148972	39.0	35.0	41.0	26.0	41.0
50-51	35.30898348118976	39.0	35.0	41.0	26.0	41.0
52-53	35.10673617198886	39.0	34.5	41.0	26.0	41.0
54-55	34.893201806310095	39.0	34.0	41.0	26.0	41.0
56-57	34.63211882603736	38.0	34.0	40.5	25.0	41.0
58-59	34.38610275279103	38.0	34.0	40.0	24.0	41.0
60-61	34.100404302103044	37.0	34.0	40.0	24.0	41.0
62-63	33.78408846656356	37.0	33.0	40.0	23.0	41.0
64-65	33.45461946560515	36.0	33.0	39.5	23.0	41.0
66-67	33.112379253671534	36.0	33.0	39.0	22.0	41.0
68-69	32.732808130261056	35.0	32.5	39.0	22.0	40.5
70-71	32.38317731319609	35.0	32.0	37.5	21.5	40.0
72-73	32.01037925724398	35.0	32.0	37.0	20.0	39.0
74-75	31.6795715798902	35.0	32.0	36.5	20.0	39.0
76-77	31.473043582500473	35.0	32.0	36.0	20.0	39.0
78-79	31.333464687026588	35.0	32.0	36.0	20.0	37.5
80-81	31.100833393039558	35.0	32.0	35.0	19.5	37.0
82-83	30.899570492277654	35.0	32.0	35.0	19.0	37.0
84-85	30.67704704857083	35.0	31.0	35.0	18.0	36.0
86-87	30.531046475788017	35.0	31.0	35.0	18.0	36.0
88-89	30.398878627798148	35.0	31.0	35.0	17.0	36.0
90-91	30.254886367312558	35.0	31.0	35.0	15.0	35.0
92-93	30.145337684842033	35.0	31.0	35.0	12.5	35.0
94-95	30.064811288306835	35.0	31.0	35.0	10.5	35.0
96-97	30.006328892844166	35.0	31.0	35.0	10.0	35.0
98-99	29.908137723503884	34.0	31.0	35.0	7.0	35.0
100-101	29.806766875211743	34.0	31.0	35.0	7.0	35.0
102-103	28.340786720965262	32.5	28.0	34.0	7.0	35.0
104-105	29.452765264830678	34.0	30.0	35.0	6.0	35.0
106-107	29.638493565322943	34.0	31.0	35.0	3.5	35.0
108-109	29.741655997280176	34.5	31.0	35.0	2.0	35.0
110-111	29.931037028642514	35.0	31.0	35.0	2.0	35.0
112-113	29.905592650597125	35.0	31.0	35.0	2.0	35.0
114-115	29.811988606266198	35.0	31.0	35.0	2.0	35.0
116-117	29.68158485392351	35.0	31.0	35.0	2.0	35.0
118-119	29.566727312312906	35.0	31.0	35.0	2.0	35.0
120-121	29.365579623062864	35.0	31.0	35.0	2.0	35.0
122-123	29.15400623471909	35.0	31.0	35.0	2.0	35.0
124-125	28.368068032943548	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89552.0
3	34139.0
4	15397.0
5	12821.0
6	13350.0
7	15746.0
8	17697.0
9	19415.0
10	21494.0
11	22658.0
12	23136.0
13	23890.0
14	24660.0
15	22700.0
16	18753.0
17	17618.0
18	17566.0
19	17022.0
20	17638.0
21	18670.0
22	20170.0
23	23672.0
24	31401.0
25	41556.0
26	52980.0
27	64752.0
28	78458.0
29	94908.0
30	114176.0
31	140264.0
32	173990.0
33	223104.0
34	301571.0
35	449140.0
36	862168.0
37	1448857.0
38	453060.0
39	410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54130812716068	17.545521090676182	11.31972375053209	26.59344703163104
2	17.407533555954643	20.21568622780734	35.77115180328025	26.605628412957767
3	19.499024914786723	23.387794941941443	29.0385060416383	28.07467410163353
4	13.974490145272997	15.522844845979266	34.51975758393711	35.98290742481063
5	14.620067395607128	36.7801673209968	33.94171734050355	14.658047942892512
6	34.958506203467046	34.281316641424134	16.467946685073493	14.292230470035324
7	29.807350238941133	30.883955130841905	20.78043382625033	18.528260803966628
8	27.959226330903746	33.5102637858366	19.623142106185664	18.907367777073983
9	27.371957855856454	14.065892752329217	18.388011718484044	40.174137673330286
10-11	26.471316531709206	24.59807276597176	27.372120242269393	21.558490460049637
12-13	26.584116215483654	21.961527822839454	27.2904594094086	24.163896552268294
14-15	24.04619730041635	23.41726186334617	24.137701376710044	28.398839459527437
16-17	24.49145374692458	26.173576866052606	25.03200855930564	24.302960827717175
18-19	24.469551488680242	25.7633232615984	26.018694894919754	23.748430354801602
20-21	25.294809898692765	25.03101864731602	25.561330572679513	24.112840881311705
22-23	24.946018403168985	24.87160141235469	25.42567947933117	24.756700705145153
24-25	24.595623843953685	25.02785626870007	25.676755728982698	24.699764158363546
26-27	24.496475442229627	25.124242323792455	25.714523623459364	24.664758610518554
28-29	24.607512180588017	25.237376438652227	25.676533643493237	24.478577737266523
30-31	24.773280680190215	25.27430266261281	25.457718609706927	24.49469804749005
32-33	24.474086954450446	25.332465892917753	25.458681450868532	24.734765701763266
34-35	24.691513397693928	25.186048755195372	25.635096705651712	24.487341141458987
36-37	24.72815449566897	25.080306034376658	25.59917373417998	24.592365735774386
38-39	24.67862981310851	25.208465055986057	25.56015337231497	24.552751758590468
40-41	24.73501533638506	25.104454370705966	25.669779470108097	24.49075082280088
42-43	24.58537751261843	24.99099747214576	25.63082211441687	24.79280290081894
44-45	24.587954475367688	25.046736225852868	25.57391390569342	24.791395393086027
46-47	24.54628710701946	25.011987415769237	25.719560077970062	24.72216539924124
48-49	24.46107308670065	25.159129999018386	25.582464281542112	24.79733263273885
50-51	24.311599992457033	25.32575198920959	25.642209255816766	24.72043876251661
52-53	24.508476924567162	25.104358710513402	25.551170802507073	24.835993562412366
54-55	24.247024768083023	25.068303414351877	25.66625294719295	25.018418870372145
56-57	24.228760242642245	25.2160241550425	25.579818787553045	24.97539681476221
58-59	24.224253850890474	25.11942038237549	25.6496957122604	25.00663005447364
60-61	24.331329549419088	25.115996309715875	25.75636680709531	24.796307333769725
62-63	24.098415476758035	25.202270323141178	25.67275460362203	25.02655959647876
64-65	24.0093449011124	25.096317929114647	25.66455326515224	25.22978390462071
66-67	24.044289039661702	25.239813604253257	25.518625083226564	25.197272272858473
68-69	24.143908643623845	25.067264444921065	25.537224787457852	25.251602123997234
70-71	23.893393550763992	24.93497089173249	25.70697320070254	25.464662356800982
72-73	23.99021947422031	25.010198800680595	25.57883032709415	25.42075139800495
74-75	23.859069541615042	25.051172948288325	25.584002836148507	25.50575467394813
76-77	23.894312353026063	24.963769503976174	25.606936485027543	25.534981657970224
78-79	23.848623223100667	25.034142510840248	25.554468420956518	25.562765845102575
80-81	23.78415117770622	25.14192065623937	25.54207556900268	25.531852597051735
82-83	23.747233227118635	24.922155203676763	25.697536418383105	25.6330751508215
84-85	23.707306305614228	24.978368177489923	25.485859963947956	25.828465552947893
86-87	23.564636896349114	25.0242571977577	25.63808141843071	25.773024487462482
88-89	23.587592701054056	24.84863174291331	25.564841334662923	25.99893422136971
90-91	23.492617579952523	24.94785257411326	25.586504086391095	25.973025759543127
92-93	23.456527668108937	24.917118145002654	25.612378902771678	26.013975284116736
94-95	23.385684642999387	24.80182291850173	25.688708221186573	26.123784217312306
96-97	23.346440211258336	24.695765801549204	25.742092848623006	26.215701138569454
98-99	23.381332378433935	24.741647177641063	25.597642499480934	26.27937794444407
100-101	23.31312486873861	24.725775350371144	25.711769238801047	26.2493305420892
102-103	23.106836397925505	24.67646343581727	25.73621181667039	26.48048834958684
104-105	23.068960719346407	24.84088835359317	25.561463392461764	26.528687534598657
106-107	23.03251164745562	24.834920979909658	25.630446292687697	26.50212107994702
108-109	22.887502068106226	24.997382301060092	25.618553575191182	26.496562055642503
110-111	22.84519946331148	25.163283780257828	25.487930909739443	26.503585846691248
112-113	22.746913200422544	25.28898986237973	25.420498659604696	26.543598277593027
114-115	22.75411847578436	25.419310544953355	25.34677487703473	26.479796102227553
116-117	22.597672064325327	25.38541319225739	25.421804243929014	26.59511049948827
118-119	22.64754905599487	25.476587331043465	25.288046306525093	26.587817306436573
120-121	22.676412808890376	25.619874651628145	25.183935973807753	26.519776565673723
122-123	22.52795066127628	25.741888445842708	25.065086638788532	26.66507425409248
124-125	22.40651181022271	25.910455166801878	25.065983928868246	26.61704909410717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	61.0
2	155.5
3	240.0
4	188.5
5	132.0
6	178.0
7	309.0
8	454.5
9	502.0
10	446.0
11	378.0
12	395.5
13	452.0
14	484.5
15	531.5
16	599.5
17	681.5
18	794.0
19	884.0
20	989.5
21	1178.0
22	1407.0
23	1675.5
24	2050.0
25	2644.0
26	3405.0
27	4540.5
28	6242.5
29	8821.5
30	12375.5
31	16891.0
32	22824.0
33	29219.0
34	36730.0
35	45667.0
36	57062.0
37	69947.5
38	83549.5
39	96487.5
40	109962.0
41	125334.5
42	139531.5
43	155539.5
44	172397.0
45	188234.0
46	205245.5
47	218984.0
48	229989.0
49	240043.5
50	248237.5
51	252699.5
52	249144.0
53	246193.0
54	243023.5
55	231493.0
56	214306.5
57	191956.0
58	170021.5
59	150274.5
60	127289.5
61	104379.5
62	83094.5
63	62438.5
64	44720.0
65	32372.0
66	24335.5
67	18506.5
68	13726.5
69	9888.0
70	7201.0
71	5274.5
72	3668.0
73	2367.5
74	1599.5
75	1115.5
76	841.5
77	543.5
78	326.0
79	209.5
80	130.5
81	78.0
82	42.0
83	31.5
84	27.5
85	23.5
86	15.5
87	9.5
88	11.5
89	14.0
90	10.5
91	6.0
92	4.5
93	6.0
94	7.5
95	9.5
96	13.5
97	11.0
98	5.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22266286849077285
2	0.008077706344214685
3	4.961736083669954E-4
4	0.002262551654153499
5	0.08738609590559523
6	0.054499709143030775
7	0.026495670686797555
8	0.01583786157907449
9	0.014011942700283948
10-11	0.012176100349326067
12-13	0.014101253949790008
14-15	0.02140492946495218
16-17	0.024501052781162234
18-19	0.023707175007775038
20-21	0.01713783643299602
22-23	0.014508116308650947
24-25	0.012791355623701144
26-27	0.012344799376170845
28-29	0.014438652003479567
30-31	0.011044824522249317
32-33	0.014865361306675182
34-35	0.017882096845546515
36-37	0.018953831839619226
38-39	0.02270490431887371
40-41	0.02365755764693834
42-43	0.0215339346031276
44-45	0.0194202350314842
46-47	0.01953931669749228
48-49	0.01850727559208893
50-51	0.014964596028348582
52-53	0.01284097298453784
54-55	0.01838819392608085
56-57	0.021861409184649818
58-59	0.017723321290869076
60-61	0.019975949472855234
62-63	0.02585064499592046
64-65	0.018765285868439768
66-67	0.021930873489821198
68-69	0.01574855032956843
70-71	0.01617525963276405
72-73	0.02385602709028514
74-75	0.014706585751997743
76-77	0.013416534370243555
78-79	0.016889749628812522
80-81	0.017564545736191636
82-83	0.01290051381754188
84-85	0.016711127129800406
86-87	0.017445464070183558
88-89	0.018408040870415532
90-91	0.013466151731080254
92-93	0.013198217982562079
94-95	0.013962325339447253
96-97	0.014805820473671143
98-99	0.013515769091916954
100-101	0.017882096845546515
102-103	0.023498782092260903
104-105	0.00853418606391232
106-107	0.008762425923761138
108-109	0.014637121446826364
110-111	0.012453957570011585
112-113	0.00978454355699715
114-115	0.006906736628468576
116-117	0.004614414557813058
118-119	0.0026396435965124155
120-121	0.0024014802644962575
122-123	0.0016373729076110848
124-125	4.26709303195616E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	5038559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.225622611746644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.51248824070932	30.300074491253405
2	14.347580788846997	12.690613867181872
3	6.145405398914446	8.15353139905742
4	3.248243399163136	5.746223468899438
5	1.9105907952211707	4.224853373746421
6	1.2523152688118422	3.323065348164035
7	0.8489167156720749	2.6280709207281636
8	0.6217885753626171	2.1999189502626155
9	0.46432258822191824	1.8481460001130796
>10	2.43537366810059	19.316264140409075
>50	0.15227042706077468	4.596187016181901
>100	0.058862798698795696	4.2395477628622755
>500	0.0013922296556226213	0.40012004362212905
>1k	4.4910556075116235E-4	0.3333832175180602
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.9846944334679816E-5	0.0
10-11	0.0	0.0	0.0	3.969388866935963E-5	0.0
12-13	0.0	0.0	0.0	3.969388866935963E-5	4.961736083669954E-5
14-15	0.0	1.9846944334679816E-5	0.0	3.969388866935963E-5	7.938777733871926E-5
16-17	0.0	1.9846944334679816E-5	0.0	3.969388866935963E-5	9.923472167339908E-5
18-19	0.0	1.9846944334679816E-5	0.0	8.931124950605916E-5	9.923472167339908E-5
20-21	0.0	1.9846944334679816E-5	0.0	1.3892861034275872E-4	1.190816660080789E-4
22-23	0.0	1.9846944334679816E-5	0.0	2.1831638768147799E-4	1.3892861034275872E-4
24-25	0.0	1.9846944334679816E-5	0.0	3.969388866935963E-4	1.3892861034275872E-4
26-27	0.0	1.9846944334679816E-5	0.0	9.129594393952715E-4	1.3892861034275872E-4
28-29	0.0	1.9846944334679816E-5	0.0	0.004564797196976358	1.3892861034275872E-4
30-31	0.0	1.9846944334679816E-5	0.0	0.020055337250193955	1.3892861034275872E-4
32-33	0.0	1.9846944334679816E-5	0.0	0.052505091237395454	1.3892861034275872E-4
34-35	0.0	1.9846944334679816E-5	0.0	0.10496056511395421	1.3892861034275872E-4
36-37	0.0	1.9846944334679816E-5	0.0	0.19565117725127362	1.3892861034275872E-4
38-39	0.0	1.9846944334679816E-5	0.0	0.3404346361727629	1.3892861034275872E-4
40-41	0.0	1.9846944334679816E-5	0.0	0.5048467230412504	1.3892861034275872E-4
42-43	0.0	1.9846944334679816E-5	0.0	0.665517660902651	1.3892861034275872E-4
44-45	0.0	1.9846944334679816E-5	0.0	0.8649992984105177	1.3892861034275872E-4
46-47	0.0	1.9846944334679816E-5	0.0	1.105772503606686	1.3892861034275872E-4
48-49	0.0	1.9846944334679816E-5	0.0	1.3676033167419495	1.3892861034275872E-4
50-51	0.0	1.9846944334679816E-5	0.0	1.6439918635467006	1.3892861034275872E-4
52-53	0.0	1.9846944334679816E-5	0.0	1.9149721180202515	1.3892861034275872E-4
54-55	0.0	1.9846944334679816E-5	0.0	2.223403556453343	1.3892861034275872E-4
56-57	0.0	1.9846944334679816E-5	0.0	2.5850446526477118	1.3892861034275872E-4
58-59	0.0	1.9846944334679816E-5	0.0	2.9845735655769836	1.3892861034275872E-4
60-61	0.0	1.9846944334679816E-5	0.0	3.4025204428488385	1.3892861034275872E-4
62-63	0.0	1.9846944334679816E-5	0.0	3.8211222692837374	1.3892861034275872E-4
64-65	0.0	1.9846944334679816E-5	0.0	4.268899103890616	1.3892861034275872E-4
66-67	0.0	1.9846944334679816E-5	0.0	4.786914274497927	1.3892861034275872E-4
68-69	0.0	1.9846944334679816E-5	0.0	5.3663755847654055	1.3892861034275872E-4
70-71	0.0	1.9846944334679816E-5	0.0	5.98069805275675	1.3892861034275872E-4
72-73	0.0	1.9846944334679816E-5	0.0	6.594266336863377	1.4885208251009862E-4
74-75	0.0	1.9846944334679816E-5	0.0	7.2117246220596005	1.6869902684477843E-4
76-77	0.0	1.9846944334679816E-5	0.0	7.895253384945973	1.7862249901211835E-4
78-79	0.0	1.9846944334679816E-5	0.0	8.662575946813364	1.7862249901211835E-4
80-81	0.0	1.9846944334679816E-5	0.0	9.467032141530941	1.7862249901211835E-4
82-83	0.0	1.9846944334679816E-5	0.0	10.301943472330084	1.7862249901211835E-4
84-85	0.0	1.9846944334679816E-5	0.0	11.123438268758985	1.7862249901211835E-4
86-87	0.0	1.9846944334679816E-5	0.0	11.988507031474672	1.7862249901211835E-4
88-89	0.0	1.9846944334679816E-5	0.0	12.931891836535009	1.7862249901211835E-4
90-91	0.0	1.9846944334679816E-5	0.0	13.933884668215654	1.7862249901211835E-4
92-93	0.0	1.9846944334679816E-5	0.0	14.95206268300123	1.7862249901211835E-4
94-95	0.0	1.9846944334679816E-5	0.0	15.954998244537773	1.7862249901211835E-4
96-97	0.0	1.9846944334679816E-5	0.0	16.98142266469441	2.0839291551413809E-4
98-99	0.0	1.9846944334679816E-5	0.0	18.087939428713646	2.381633320161578E-4
100-101	0.0	1.9846944334679816E-5	0.0	19.264426594984798	2.381633320161578E-4
102-103	0.0	1.9846944334679816E-5	0.0	20.44992427398389	2.381633320161578E-4
104-105	0.0	1.9846944334679816E-5	0.0	21.626024424840516	2.381633320161578E-4
106-107	0.0	1.9846944334679816E-5	0.0	22.785016509680645	2.381633320161578E-4
108-109	0.0	1.9846944334679816E-5	0.0	23.996781619506688	2.381633320161578E-4
110-111	0.0	1.9846944334679816E-5	0.0	25.255901538515275	2.381633320161578E-4
112-113	0.0	1.9846944334679816E-5	0.0	26.54799715553594	2.381633320161578E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	19810	0.0	32.110737	1
CATGGGG	14135	0.0	30.964659	4
TACATGG	20360	0.0	30.74154	2
GTATCAA	9520	0.0	30.212564	1
ACATGGG	19955	0.0	30.169922	3
GGTATCA	7465	0.0	29.416807	1
TCAACGC	11165	0.0	25.334343	4
CAACGCA	11480	0.0	24.691065	5
TATCAAC	11955	0.0	24.010328	2
ATGGGGG	7105	0.0	23.886665	5
AACGCAG	12085	0.0	23.539732	6
ATCAACG	12050	0.0	23.523108	3
ATGGGGA	7450	0.0	21.181875	5
GTATATA	1185	0.0	21.149906	1
ACGCAGA	14205	0.0	19.771477	7
TATACCG	500	1.8189894E-12	19.055693	5
CGCAGAG	15485	0.0	17.86622	8
GTATAAT	1585	0.0	17.694818	1
ACCGTCG	445	1.6043487E-9	17.38098	8
GAGTACA	14615	0.0	17.148573	1
>>END_MODULE