FastQCFastQC Report
Mon 15 Aug 2016
SRR2920935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093168
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG48800.033.5654454
GTACATG79800.032.4843251
TACATGG81200.031.2495332
ACATGGG82500.029.7463193
ATGGGGA24200.028.7971845
GTATCAA49500.025.5222031
GGTATCA37950.025.2814271
GTACTAG2701.6825652E-924.2781941
AACGCAG53350.022.3143466
TATCAAC59250.021.312652
TCAACGC55750.021.26124
CAACGCA57800.020.6101765
CCCTACG2909.519863E-820.5489431
ATGGGGG27250.020.5466445
GTATTAG6150.020.3484651
ATAGGAC6200.020.1751863
GTATAGT4152.910383E-1120.1033041
ATCAACG59200.020.0226383
GTATAAT7350.019.458591
ACGCAGA61100.019.384237
Produced by FastQC (version 0.11.5)