##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920935_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2093168 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.844259992508963 31.0 30.0 34.0 25.0 34.0 2 29.976407053805524 31.0 30.0 34.0 25.0 34.0 3 30.06877995459514 31.0 31.0 34.0 25.0 34.0 4 33.467246298433764 37.0 35.0 37.0 27.0 37.0 5 33.583948350060766 37.0 35.0 37.0 28.0 37.0 6 33.54697234049058 37.0 35.0 37.0 28.0 37.0 7 33.45717639482354 37.0 35.0 37.0 27.0 37.0 8 33.37634437369576 37.0 35.0 37.0 27.0 37.0 9 34.82454490036156 39.0 35.0 39.0 27.0 39.0 10-11 34.82819343693387 39.0 35.0 39.0 27.0 39.0 12-13 34.77316393141879 39.0 35.0 39.0 27.0 39.0 14-15 35.93240126927222 40.0 36.0 41.0 27.0 41.0 16-17 35.913180404057385 40.0 36.0 41.0 27.0 41.0 18-19 35.89210971121286 40.0 36.0 41.0 27.0 41.0 20-21 35.90785999977068 40.0 36.0 41.0 27.0 41.0 22-23 35.806627322794924 40.0 36.0 41.0 27.0 41.0 24-25 35.7864089265649 40.0 36.0 41.0 27.0 41.0 26-27 35.69014121178998 40.0 36.0 41.0 26.5 41.0 28-29 35.558353414537194 40.0 35.5 41.0 26.0 41.0 30-31 35.39070323070102 40.0 35.0 41.0 25.0 41.0 32-33 35.23823362482132 40.0 35.0 41.0 25.0 41.0 34-35 35.154522475023505 40.0 35.0 41.0 24.5 41.0 36-37 35.011433148223176 39.5 35.0 41.0 24.0 41.0 38-39 34.86582515115843 39.0 35.0 41.0 23.5 41.0 40-41 34.71781935324828 39.0 34.5 41.0 22.5 41.0 42-43 34.529575743561914 39.0 34.0 41.0 21.5 41.0 44-45 34.3834178623025 39.0 34.0 41.0 21.0 41.0 46-47 34.17750605780329 39.0 33.5 41.0 19.0 41.0 48-49 34.04484685414644 39.0 33.0 41.0 18.5 41.0 50-51 33.83085136978971 38.0 33.0 40.0 17.0 41.0 52-53 33.620651806257314 38.0 33.0 40.0 14.5 41.0 54-55 33.36686352934881 38.0 33.0 40.0 13.0 41.0 56-57 33.094295345619656 38.0 32.5 40.0 10.0 41.0 58-59 32.81751990284583 37.0 32.0 40.0 8.0 41.0 60-61 32.533398895836356 37.0 32.0 40.0 7.5 41.0 62-63 32.206038884599806 36.0 31.0 39.5 7.0 41.0 64-65 31.902375251293734 36.0 31.0 39.0 4.0 41.0 66-67 31.544940492115302 35.0 31.0 39.0 2.0 40.5 68-69 31.2066489646316 35.0 30.5 38.5 2.0 40.0 70-71 30.84531461402047 35.0 30.0 37.5 2.0 40.0 72-73 30.913812221474817 35.0 31.0 37.0 2.0 39.5 74-75 30.90844929790633 35.0 31.0 37.0 2.0 39.0 76-77 30.650449939995262 35.0 31.0 36.5 2.0 39.0 78-79 30.355229250590494 35.0 31.0 36.0 2.0 38.5 80-81 30.091526098239605 35.0 31.0 35.5 2.0 37.0 82-83 29.863807157380585 35.0 31.0 35.0 2.0 37.0 84-85 29.6382968304503 35.0 30.5 35.0 2.0 36.5 86-87 29.446104899367846 35.0 30.0 35.0 2.0 36.0 88-89 29.248886377013214 35.0 30.0 35.0 2.0 36.0 90-91 29.07125371685407 34.5 30.0 35.0 2.0 35.5 92-93 28.897738021983905 34.0 30.0 35.0 2.0 35.0 94-95 28.773581719193107 34.0 29.0 35.0 2.0 35.0 96-97 28.67343686698822 34.0 29.0 35.0 2.0 35.0 98-99 28.520964394640085 34.0 29.0 35.0 2.0 35.0 100-101 28.373427742063704 34.0 29.0 35.0 2.0 35.0 102-103 27.01427047422854 32.5 26.0 34.5 2.0 35.0 104-105 28.016341736544796 34.0 29.0 35.0 2.0 35.0 106-107 28.155288299840244 34.0 29.0 35.0 2.0 35.0 108-109 28.058495543597076 34.0 29.0 35.0 2.0 35.0 110-111 27.92785720018651 34.0 29.0 35.0 2.0 35.0 112-113 27.756541758712153 34.0 28.5 35.0 2.0 35.0 114-115 27.591021360922774 34.0 27.5 35.0 2.0 35.0 116-117 27.314967551577322 34.0 27.0 35.0 2.0 35.0 118-119 27.036571598648557 34.0 26.5 35.0 2.0 35.0 120-121 26.779155089319154 34.0 25.0 35.0 2.0 35.0 122-123 26.405789215199164 34.0 25.0 35.0 2.0 35.0 124-125 25.034325242885426 32.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75189.0 3 11332.0 4 7968.0 5 8784.0 6 12791.0 7 12298.0 8 10436.0 9 10910.0 10 11583.0 11 12012.0 12 12119.0 13 11944.0 14 11925.0 15 10925.0 16 8904.0 17 8649.0 18 8735.0 19 9096.0 20 9623.0 21 10693.0 22 12367.0 23 15207.0 24 19003.0 25 22847.0 26 26232.0 27 29708.0 28 34693.0 29 41457.0 30 49074.0 31 59830.0 32 74676.0 33 95836.0 34 129849.0 35 190383.0 36 336544.0 37 510607.0 38 168784.0 39 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.921543741972044 18.198463608815587 11.18525707656879 25.694735572643584 2 17.053670113254128 21.260819429758037 35.95926296298068 25.726247494007147 3 19.211444488582792 24.34375583304218 29.214076835822894 27.23072284255213 4 13.287677918319773 16.03714634038978 35.19285130554774 35.482324435742704 5 14.037509230858323 37.676929769859086 33.87135138808658 14.414209611196007 6 33.68127844839914 35.41947354349696 16.647224784979482 14.252023223124407 7 28.33323369259797 32.512166133788014 21.0584237473366 18.096176426277413 8 26.814926469463018 34.002852081962445 20.136301207400493 19.04592024117404 9 26.498429200185754 15.161793098237041 18.848067382983903 39.491710318593306 10-11 25.32086279402212 25.899552689255913 27.521816231290426 21.257768285431546 12-13 25.54908848025218 23.691720122235612 27.241314929301907 23.517876468210304 14-15 23.209155765778668 25.220095580541464 24.238663048589444 27.332085605090423 16-17 23.348864850934962 27.761637462297763 25.486916782190843 23.402580904576432 18-19 23.457331974562003 26.901377441288243 26.737555134346003 22.90373544980375 20-21 24.192714452460457 26.379865531490527 26.117162626962116 23.310257389086907 22-23 23.848084383223743 26.187816251849412 26.08030556173021 23.88379380319664 24-25 23.530386146068626 26.460745999306823 26.2711985945287 23.73766926009585 26-27 23.304201776292686 26.49696751798321 26.237788376238612 23.961042329485487 28-29 23.520301497399476 26.833885278575774 26.15453128159821 23.491281942426543 30-31 23.555434126304586 26.763915242382826 26.002281340100865 23.678369291211727 32-33 23.18777662126297 26.88516031064296 25.931720895630146 23.99534217246393 34-35 23.619357065745902 26.70283677838667 26.016352090602496 23.66145406526493 36-37 23.69105892410274 26.486633281346073 26.0451877996001 23.777119994951086 38-39 23.432711980729056 26.582670637700968 26.161638689902905 23.822978691667068 40-41 23.432237749265138 26.486602267793995 26.43998607729777 23.641173905643093 42-43 23.30640655804955 26.539914555638884 26.218787001804657 23.934891884506918 44-45 23.19092924209317 26.489412739994506 26.34276088432862 23.976897133583705 46-47 23.22345266221554 26.64357281018705 26.26771599337207 23.865258534225344 48-49 22.999466043063602 26.69127393902948 26.19312127706055 24.116138740846367 50-51 22.901542118311617 26.701574435007586 26.323430847529256 24.073452599151544 52-53 23.27457993045971 26.346691768611446 26.167725674881687 24.211002626047158 54-55 22.93224509621595 26.389718224545476 26.274524896244735 24.403511782993842 56-57 22.887016546058167 26.480458172054526 26.21059506142619 24.421930220461118 58-59 22.922354762201255 26.01749935644799 26.38869203221656 24.6714538491342 60-61 22.875149550660772 26.06887039500498 26.506365091260264 24.549614963073985 62-63 22.89035153557186 25.90097304036631 26.668591649082078 24.540083774979752 64-65 22.90108630416373 25.968905442611163 26.40118991763267 24.728818335592432 66-67 22.975194956526934 26.16235919806388 26.22646628223132 24.635979563177866 68-69 23.38160149520629 25.71973774131392 26.366803131251487 24.531857632228302 70-71 23.149791871080776 25.698202908665884 26.405040508353245 24.746964711900098 72-73 23.19637697924813 25.672887001447446 26.444557151297637 24.686178868006785 74-75 23.128396600298437 25.705181846364255 26.485911421229467 24.680510132107838 76-77 23.22821103247516 25.453164676784247 26.65627323909927 24.662351051641327 78-79 23.333693465090562 25.677505835807647 26.557502126131844 24.431298572969943 80-81 23.53874200147667 25.52136951116238 26.44472972872843 24.495158758632527 82-83 23.33986844677994 25.379521813271683 26.63668259092628 24.643927149022097 84-85 23.384143936550885 25.35265841362875 26.545365016457474 24.717832633362892 86-87 23.426123715889 25.2475362302213 26.60233174647223 24.72400830741747 88-89 23.601921516868245 25.183458586951797 26.32881094551384 24.88580895066612 90-91 23.602722607798576 25.2633116265373 26.35312919192779 24.78083657373634 92-93 23.779291144795252 24.99203494537753 26.482725093160663 24.74594881666655 94-95 23.513329070580433 24.905456522908313 26.66966254534155 24.911551861169706 96-97 23.701729911447824 24.72042287839617 26.55284175496351 25.025005455192495 98-99 23.658735712619546 24.983724145051568 26.376946888611013 24.980593253717874 100-101 23.738867581096017 25.084580572136545 26.43516907510583 24.741382771661613 102-103 23.531807267672026 25.08706929288956 26.514950729044756 24.86617271039366 104-105 23.328122651219914 25.251669732453262 26.389623437601944 25.030584178724887 106-107 23.351143733635126 25.356624285801356 26.316688914728775 24.975543065834742 108-109 23.26364962875591 25.58442412052855 26.3489013254364 24.803024925279136 110-111 23.492203191962084 25.857933330162197 26.105831596977797 24.544031880897922 112-113 23.536614952893498 26.030397229183922 26.095651955669812 24.337335862252772 114-115 23.38125601324258 26.258361768625367 25.991000304770555 24.369381913361497 116-117 23.410588467307704 26.171406715949637 26.045215229512408 24.372789587230255 118-119 23.57356582132099 26.280698936374147 25.765235511689177 24.380499730615682 120-121 23.67826873055188 26.507673119056808 25.7003953086912 24.11366284170012 122-123 23.90028508891968 26.622003453001202 25.550870337486288 23.92684112059283 124-125 24.034075805183853 26.72585500283997 25.28803694063604 23.952032251340142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1147.0 1 888.0 2 538.5 3 386.5 4 325.5 5 277.0 6 246.0 7 264.5 8 272.5 9 287.5 10 284.0 11 290.0 12 323.5 13 359.5 14 407.0 15 450.0 16 506.5 17 604.5 18 709.0 19 817.5 20 1035.5 21 1312.5 22 1679.0 23 2190.0 24 2748.0 25 3487.0 26 4660.5 27 6350.5 28 8351.5 29 10908.5 30 13860.0 31 17617.5 32 22988.5 33 26352.5 34 27850.0 35 30845.5 36 35408.5 37 39913.0 38 44706.5 39 48684.0 40 52410.0 41 57496.5 42 61316.5 43 66643.0 44 71587.5 45 75894.0 46 80936.5 47 84084.5 48 87226.5 49 90707.0 50 94011.5 51 95333.0 52 93663.5 53 91721.5 54 88400.5 55 82389.5 56 75477.5 57 68105.5 58 61859.0 59 54598.5 60 44483.5 61 35484.0 62 28829.5 63 22154.0 64 15933.0 65 11842.0 66 9080.5 67 7122.0 68 5550.5 69 4155.5 70 3229.0 71 2542.5 72 2020.5 73 1719.0 74 1436.5 75 1129.5 76 815.5 77 547.5 78 376.0 79 259.5 80 177.0 81 138.5 82 96.5 83 63.5 84 52.0 85 43.0 86 40.0 87 36.5 88 25.0 89 24.5 90 20.5 91 11.5 92 9.5 93 11.5 94 10.0 95 7.5 96 7.0 97 8.0 98 7.0 99 8.5 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17179700817134602 2 0.1518272780780138 3 0.18049196242250978 4 0.1511584354433089 5 0.17719552372289277 6 0.11088455393929202 7 0.11098010288710701 8 0.09860651414506623 9 0.10486497022694787 10-11 0.09041319186993113 12-13 0.07803960312789035 14-15 0.07803960312789035 16-17 0.07713188812364799 18-19 0.07512536021953327 20-21 0.06934464887672658 22-23 0.06129465002331395 24-25 0.06416111845776354 26-27 0.07474316442827332 28-29 0.07046734901355266 30-31 0.06760088057910306 32-33 0.0781112648387516 34-35 0.07500592403476453 36-37 0.07787239246921412 38-39 0.0726888620502511 40-41 0.0773946477301392 42-43 0.07794405418007537 44-45 0.07068233414613638 46-47 0.06755310610519558 48-49 0.05919257317138424 50-51 0.06545102925326586 52-53 0.06836527216162296 54-55 0.07008515322229271 56-57 0.07414598350442965 58-59 0.06031527330821033 60-61 0.07096898098958133 62-63 0.06564212714889583 64-65 0.060124175412580354 66-67 0.06475829938160721 68-69 0.0676725422899643 70-71 0.06284732042530748 72-73 0.057902662375881916 74-75 0.06107966489073023 76-77 0.0698223936158015 78-79 0.06406556950994856 80-81 0.0619873798949726 82-83 0.06645429320532321 84-85 0.06750533163128808 86-87 0.06222625226451006 88-89 0.06110355212768397 90-91 0.06449553977511599 92-93 0.05816542198237313 94-95 0.06714702307698188 96-97 0.053722395908976255 98-99 0.053125214985132584 100-101 0.0515964318200928 102-103 0.048371654831337 104-105 0.04736839087927964 106-107 0.05854761777363308 108-109 0.05806987303455815 110-111 0.06604821017710953 112-113 0.06523604412068215 114-115 0.06834138492466921 116-117 0.0719244704677312 118-119 0.06502105898809843 120-121 0.06616764636187826 122-123 0.06518826964677465 124-125 0.07624806035635937 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2093168.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.65888577557756 #Duplication Level Percentage of deduplicated Percentage of total 1 70.23978564244821 34.177897064769404 2 13.619358001272985 13.25405570641281 3 5.983493884221694 8.734504363536308 4 3.1752533538301013 6.180171610101539 5 1.8503642853410143 4.50183322018083 6 1.259390681152003 3.6768328380601276 7 0.8221038723677996 2.800186089484333 8 0.6022771823152895 2.3444909295613097 9 0.44258550918941203 1.9382145963816086 >10 1.8893320544462526 15.325395527692043 >50 0.07852676261361789 2.6309494195622287 >100 0.03387652575463494 2.9238895024584015 >500 0.0026651517909958805 0.896928242102798 >1k 9.870932559243862E-4 0.6146508896963364 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8664684344495997E-4 2 0.0 0.0 0.0 0.0 2.8664684344495997E-4 3 0.0 0.0 0.0 0.0 3.344213173524533E-4 4 0.0 0.0 0.0 0.0 3.344213173524533E-4 5 0.0 0.0 0.0 0.0 3.344213173524533E-4 6 0.0 0.0 0.0 0.0 5.732936868899199E-4 7 0.0 0.0 0.0 0.0 5.732936868899199E-4 8 0.0 0.0 0.0 0.0 5.732936868899199E-4 9 0.0 0.0 0.0 0.0 5.732936868899199E-4 10-11 0.0 0.0 0.0 0.0 6.688426347049066E-4 12-13 0.0 0.0 0.0 0.0 0.0013137980324560666 14-15 0.0 0.0 0.0 0.0 0.0016004448759010265 16-17 0.0 0.0 0.0 4.777447390749333E-5 0.001982640667160973 18-19 0.0 0.0 0.0 7.166171086124E-5 0.0020543023780222134 20-21 0.0 0.0 0.0 2.3887236953746665E-4 0.0024603854062359067 22-23 0.0 0.0 0.0 0.0010510384259648532 0.0026275960649121332 24-25 0.0 0.0 0.0 0.0027948067235883597 0.0026275960649121332 26-27 0.0 0.0 0.0 0.005040206997240546 0.0028186939605421065 28-29 0.0 0.0 0.0 0.021044655756250814 0.0028186939605421065 30-31 0.0 0.0 0.0 0.07271274928720485 0.0028186939605421065 32-33 0.0 0.0 0.0 0.1619554665464024 0.0028186939605421065 34-35 0.0 0.0 0.0 0.3059716181405411 0.00300979185617208 36-37 0.0 0.0 0.0 0.5364356802702889 0.0030336790931258266 38-39 0.0 0.0 0.0 0.8748222789570641 0.0030575663300795733 40-41 0.0 0.0 0.0 1.2383860253930883 0.0030575663300795733 42-43 0.0 0.0 0.0 1.569869212600231 0.00308145356703332 44-45 0.0 0.0 0.0 1.9734679681707346 0.003200889751802053 46-47 0.0 0.0 0.0 2.477608104079558 0.0033203259365707865 48-49 0.0 0.0 0.0 2.988866636600598 0.00343976212133952 50-51 0.0 0.0 0.0 3.5156040986676653 0.003487536595247013 52-53 0.0 0.0 0.0 4.016352246929057 0.003630860016969493 54-55 0.0 0.0 0.0 4.569508993066968 0.0036786344908769863 56-57 0.0 0.0 0.0 5.229823884179387 0.00372640896478448 58-59 0.0 0.0 0.0 5.933422448651996 0.0037741834386919733 60-61 0.0 0.0 0.0 6.644067748025959 0.00386973238650696 62-63 0.0 0.0 0.0 7.32989420820498 0.00401305580822944 64-65 0.0 0.0 0.0 8.04734737011076 0.004060830282136933 66-67 0.0 0.0 0.0 8.871027074749852 0.004108604756044427 68-69 0.0 0.0 0.0 9.785167745732783 0.004204153703859413 70-71 0.0 0.0 0.0 10.745004701008233 0.004275815414720653 72-73 0.0 0.0 0.0 11.666980385711993 0.004538575021211867 74-75 0.0 0.0 0.0 12.573214381263234 0.004896883575518067 76-77 0.0 0.0 0.0 13.591431743653638 0.0050163197602868 78-79 0.0 0.0 0.0 14.693636631173419 0.005135755945055533 80-81 0.0 0.0 0.0 15.844810354448377 0.005159643182009279 82-83 0.0 0.0 0.0 17.0078799217263 0.00530296660373176 84-85 0.0 0.0 0.0 18.132084954480483 0.00530296660373176 86-87 0.0 0.0 0.0 19.307002591287464 0.00544629002545424 88-89 0.0 0.0 0.0 20.56793816836489 0.0055896134471767195 90-91 0.0 0.0 0.0 21.88820964203542 0.005613500684130466 92-93 0.0 0.0 0.0 23.198997882635318 0.00587626029062168 94-95 0.0 0.0 0.0 24.473596959250287 0.005900147527575427 96-97 0.0 0.0 0.0 25.770984459919127 0.006354005029696613 98-99 0.0 0.0 0.0 27.142422395144585 0.0066884263470490665 100-101 0.0 0.0 0.0 28.592592663369594 0.0066884263470490665 102-103 0.0 0.0 0.0 30.04782702582879 0.006783975294864053 104-105 0.0 0.0 0.0 31.431566888085428 0.00687952424267904 106-107 0.0 0.0 0.0 32.81623835258326 0.00687952424267904 108-109 0.0 0.0 0.0 34.23506856592495 0.00687952424267904 110-111 0.0 0.0 0.0 35.696895805783385 0.0070228476644015195 112-113 0.0 0.0 0.0 37.17553966045726 0.0070228476644015195 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 4880 0.0 33.565445 4 GTACATG 7980 0.0 32.484325 1 TACATGG 8120 0.0 31.249533 2 ACATGGG 8250 0.0 29.746319 3 ATGGGGA 2420 0.0 28.797184 5 GTATCAA 4950 0.0 25.522203 1 GGTATCA 3795 0.0 25.281427 1 GTACTAG 270 1.6825652E-9 24.278194 1 AACGCAG 5335 0.0 22.314346 6 TATCAAC 5925 0.0 21.31265 2 TCAACGC 5575 0.0 21.2612 4 CAACGCA 5780 0.0 20.610176 5 CCCTACG 290 9.519863E-8 20.548943 1 ATGGGGG 2725 0.0 20.546644 5 GTATTAG 615 0.0 20.348465 1 ATAGGAC 620 0.0 20.175186 3 GTATAGT 415 2.910383E-11 20.103304 1 ATCAACG 5920 0.0 20.022638 3 GTATAAT 735 0.0 19.45859 1 ACGCAGA 6110 0.0 19.38423 7 >>END_MODULE