FastQCFastQC Report
Mon 15 Aug 2016
SRR2920935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093168
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.15727356810346804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.13099760745434672No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26660.12736674743737722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.042.575861
GTATCAA61900.039.229891
TCAACGC75050.031.8166624
CAACGCA76300.031.3734655
ATCAACG76550.031.1932143
AACGCAG78150.030.5370566
TATCAAC79550.030.3918512
GTACATG76900.027.776021
TACATGG76900.027.489912
TATACCG1107.7175786E-427.0668855
CATGGGG50250.026.8999024
TAATGCG900.00949398926.4653975
ACATGGG77900.026.2955283
ACGCAGA90250.026.1728657
CGCAGAG92050.025.4665478
ATGGGGA26100.023.9557485
GCAGAGT100700.022.864879
CGTACAC1852.6754295E-522.5313513
GAGTACT54700.022.02703712-13
AGAGTAC90650.021.59466410-11
Produced by FastQC (version 0.11.5)