##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2093168
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131302886342617	31.0	31.0	34.0	28.0	34.0
2	31.238408479395826	33.0	31.0	34.0	28.0	34.0
3	31.323305630508397	33.0	31.0	34.0	28.0	34.0
4	34.862555227291836	37.0	35.0	37.0	32.0	37.0
5	34.56914590706527	37.0	35.0	37.0	31.0	37.0
6	34.29045208029169	37.0	35.0	37.0	30.0	37.0
7	34.185684569991516	37.0	35.0	37.0	30.0	37.0
8	34.17826471644894	37.0	35.0	37.0	30.0	37.0
9	35.741710651032314	39.0	35.0	39.0	30.0	39.0
10-11	35.712976215955905	39.0	35.0	39.0	30.0	39.0
12-13	35.603084176712045	39.0	35.0	39.0	30.0	39.0
14-15	36.828401733640106	40.0	37.0	41.0	30.0	41.0
16-17	36.8325499912095	40.0	37.0	41.0	30.0	41.0
18-19	36.86007549322367	40.0	37.0	41.0	30.0	41.0
20-21	36.84919246806754	40.0	37.0	41.0	30.0	41.0
22-23	36.7904668903786	40.0	37.0	41.0	30.0	41.0
24-25	36.757357507854124	40.0	37.0	41.0	30.0	41.0
26-27	36.6180602799202	40.0	36.5	41.0	30.0	41.0
28-29	36.5142613970785	40.0	36.0	41.0	29.5	41.0
30-31	36.35923681233422	40.0	36.0	41.0	29.0	41.0
32-33	36.20187223385796	40.0	36.0	41.0	28.5	41.0
34-35	36.06861107182988	40.0	35.5	41.0	28.0	41.0
36-37	35.9238871414048	40.0	35.0	41.0	27.0	41.0
38-39	35.7853048584729	40.0	35.0	41.0	27.0	41.0
40-41	35.63893557516644	40.0	35.0	41.0	26.5	41.0
42-43	35.48356223676265	40.0	35.0	41.0	26.0	41.0
44-45	35.281308045985796	39.5	35.0	41.0	25.0	41.0
46-47	35.06860366678642	39.0	35.0	41.0	24.0	41.0
48-49	34.820504613103196	39.0	34.5	41.0	23.5	41.0
50-51	34.626095229814325	39.0	34.0	41.0	23.0	41.0
52-53	34.42303723351398	39.0	34.0	41.0	22.0	41.0
54-55	34.21522973789013	38.5	34.0	41.0	22.0	41.0
56-57	33.96457451098048	38.0	34.0	40.5	20.5	41.0
58-59	33.727764804353974	38.0	33.0	40.0	20.0	41.0
60-61	33.453545295934205	37.0	33.0	40.0	18.5	41.0
62-63	33.15029395633795	37.0	33.0	40.0	18.0	41.0
64-65	32.82778830939513	36.0	32.5	39.5	16.0	41.0
66-67	32.48553245606659	36.0	32.0	39.0	13.0	41.0
68-69	32.09637114651093	35.0	32.0	39.0	9.5	41.0
70-71	31.736377825382387	35.0	32.0	38.0	7.5	40.0
72-73	31.3661218306414	35.0	31.0	37.0	7.0	39.5
74-75	31.03614425597945	35.0	31.0	37.0	6.0	39.0
76-77	30.827310325783692	35.0	31.0	36.0	2.0	39.0
78-79	30.67431090098836	35.0	31.0	36.0	2.0	38.5
80-81	30.424431292662604	35.0	31.0	35.5	2.0	37.0
82-83	30.20675812930448	35.0	31.0	35.0	2.0	37.0
84-85	29.96756853726027	35.0	31.0	35.0	2.0	36.0
86-87	29.805753527667154	35.0	31.0	35.0	2.0	36.0
88-89	29.653257645826805	35.0	31.0	35.0	2.0	36.0
90-91	29.489059167730446	35.0	30.0	35.0	2.0	35.5
92-93	29.36359575533354	34.5	30.0	35.0	2.0	35.0
94-95	29.26614824992547	34.0	30.0	35.0	2.0	35.0
96-97	29.19620761448675	34.0	30.0	35.0	2.0	35.0
98-99	29.081972636692324	34.0	30.0	35.0	2.0	35.0
100-101	28.969210068183727	34.0	30.0	35.0	2.0	35.0
102-103	27.518061378733098	32.5	26.0	34.0	2.0	35.0
104-105	28.592889581724926	34.0	29.0	35.0	2.0	35.0
106-107	28.76505469221773	34.0	29.0	35.0	2.0	35.0
108-109	28.84201459223531	34.0	29.5	35.0	2.0	35.0
110-111	28.984935991759862	35.0	30.0	35.0	2.0	35.0
112-113	28.89965377838759	35.0	30.0	35.0	2.0	35.0
114-115	28.75091870313324	35.0	30.0	35.0	2.0	35.0
116-117	28.57251424634812	35.0	30.0	35.0	2.0	35.0
118-119	28.406287980706757	35.0	30.0	35.0	2.0	35.0
120-121	28.14322022885884	35.0	29.5	35.0	2.0	35.0
122-123	27.861844343120094	34.5	29.0	35.0	2.0	35.0
124-125	27.036237655075944	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39152.0
3	15697.0
4	6716.0
5	5882.0
6	7213.0
7	8389.0
8	8997.0
9	9775.0
10	11618.0
11	13210.0
12	13410.0
13	14687.0
14	17612.0
15	15200.0
16	9992.0
17	9258.0
18	9086.0
19	8845.0
20	9473.0
21	9970.0
22	10038.0
23	10543.0
24	13439.0
25	17169.0
26	22262.0
27	26867.0
28	32633.0
29	39143.0
30	46992.0
31	57657.0
32	72540.0
33	91909.0
34	122938.0
35	179903.0
36	333112.0
37	561447.0
38	210092.0
39	302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.084564972465586	18.573391040456755	11.54592557160074	25.796118415476926
2	17.33766395095279	21.251989962752102	35.5895640906563	25.820781995638804
3	19.207800645913355	24.3477326148025	29.89508685432552	26.54937988495863
4	13.592918104716805	16.12364746845845	34.994496233359705	35.28893819346504
5	13.898478681651607	37.44835856750491	34.21660903227781	14.43655371856567
6	33.86171506965872	35.45197503806193	16.271005767249765	14.415304125029577
7	28.24078587444589	32.010544083098694	21.355400210652277	18.393269831803142
8	27.236729564556743	33.42114190968124	20.28607470338922	19.05605382237279
9	26.675181318605183	14.076650024392423	19.206465019449436	40.04170363755296
10-11	25.575366673443533	25.190470945998733	27.856072744838574	21.378089635719164
12-13	26.046324613453876	22.830942229096383	27.76155995033594	23.3611732071138
14-15	23.351812778365407	24.334951429634312	25.059451121537375	27.253784670462906
16-17	23.23591956494729	27.064708356032153	26.17935888981277	23.520013189207788
18-19	23.38785076316517	25.892923916204154	27.886345124456813	22.832880196173864
20-21	24.031210922437147	25.270392246151435	27.492351130761616	23.206045700649803
22-23	23.69511109539616	25.292628536998272	27.411494709822676	23.60076565778289
24-25	23.44553653104125	25.52422266971732	27.665573518271074	23.364667280970355
26-27	23.23334979379037	25.565550485225124	27.638755799683167	23.562343921301338
28-29	23.429186314007207	25.760252632725717	27.5920931384881	23.21846791477898
30-31	23.38558804044968	25.67466099421311	27.52334475994441	23.416406205392796
32-33	23.15631490902523	25.837608638625255	27.380588895530604	23.625487556818914
34-35	23.54081233807377	25.597341136692258	27.453274935563993	23.40857158966998
36-37	23.611135335027917	25.41209660906843	27.40665542623833	23.570112629665317
38-39	23.358859935483824	25.43304469660171	27.50610648155559	23.701988886358873
40-41	23.325612590083967	25.392938363237477	27.719626632693483	23.561822413985073
42-43	23.512792276538054	25.29454335893756	27.42300155995505	23.76966280456934
44-45	23.269865336477228	25.441832205662017	27.408206822222315	23.880095635638444
46-47	23.35926920198822	25.502860861861514	27.2317063963462	23.906163539804066
48-49	23.199727441208605	25.659709106564797	27.00587430137099	24.134689150855603
50-51	23.01637928027496	25.762138963230118	27.11886840144933	24.10261335504559
52-53	23.26513459811451	25.530387932218712	27.082866970060614	24.121610499606163
54-55	22.86709006227652	25.620937487904744	27.01003410324055	24.501938346578182
56-57	22.80177121270441	25.750367768191236	26.769271423557377	24.678589595546978
58-59	22.648390720619428	25.493783594844917	27.057166844501236	24.800658840034423
60-61	22.659507569567293	25.728453182027998	27.01765929731708	24.594379951087628
62-63	22.395168660808686	25.76719014139194	27.079321653216414	24.758319544582964
64-65	22.245702728822447	25.69575236441003	26.887364846058006	25.17118006070952
66-67	22.350831863726928	25.9265329800703	26.758528117407316	24.964107038795458
68-69	22.76232119785017	25.470036544063117	26.80737341457634	24.960268843510374
70-71	22.53276895292973	25.409302815634486	26.803848343591934	25.25407988784385
72-73	22.59944012661664	25.497606823229873	26.658880559070557	25.24407249108293
74-75	22.534094785941797	25.549324793749363	26.554274044576704	25.362306375732143
76-77	22.644530917771448	25.41400007836114	26.606068975007574	25.335400028859834
78-79	22.60321477096447	25.651773681156207	26.494803514033894	25.25020803384543
80-81	22.66407936226546	25.685391175291468	26.3046383690999	25.34589109334317
82-83	22.45173577755634	25.487695069068682	26.46109034892341	25.59947880445157
84-85	22.47314360965559	25.46466500828078	26.274442158297166	25.78774922376646
86-87	22.485087501549962	25.466225374099615	26.279158721736017	25.769528402614405
88-89	22.461900221310454	25.444323206442782	26.056074712765888	26.037701859480883
90-91	22.364243967141803	25.43328020060472	26.071709115512082	26.130766716741398
92-93	22.459155506988704	25.231845923100117	26.218215403963065	26.090783165948118
94-95	22.278017773772625	25.10262277582962	26.395304222596938	26.224055227800825
96-97	22.383066845498536	24.930728134626566	26.320633053347102	26.365571966527796
98-99	22.324224848658456	25.186544476938437	26.106574932789517	26.382655741613593
100-101	22.33539479327065	25.104520438795902	26.187820224128515	26.37226454380493
102-103	22.07506731878881	25.023690001457478	26.329136909810742	26.572105769942965
104-105	21.924821212202293	25.350289347649095	26.078030219055666	26.646859221092946
106-107	21.94484571904174	25.635056368098457	25.936038196192186	26.48405971666762
108-109	21.84819722921168	25.801807501216057	25.92630375844429	26.423691511127974
110-111	21.811073377761268	25.939693798444985	25.89339446056374	26.35583836323001
112-113	21.667999314842852	26.24796907523613	25.842990553288285	26.24104105663273
114-115	21.55000948393536	26.443170682647455	25.701632049108152	26.30518778430903
116-117	21.507606184514444	26.557278519339928	25.759668887730037	26.175446408415592
118-119	21.547414009330634	26.70800079308014	25.498720799959866	26.245864397629358
120-121	21.42910890668114	26.930495958522933	25.460624712001312	26.17977042279461
122-123	21.40406095469055	27.064272451500234	25.29190157221594	26.239765021593275
124-125	21.207201857292425	27.333672693535043	25.24945005254032	26.209675396632214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	50.5
2	156.0
3	257.5
4	194.0
5	140.0
6	213.0
7	426.5
8	675.5
9	734.5
10	572.5
11	435.0
12	467.0
13	501.0
14	507.5
15	556.0
16	645.0
17	752.5
18	834.0
19	927.5
20	1090.0
21	1263.0
22	1576.5
23	1960.0
24	2334.5
25	3099.5
26	4370.0
27	6066.0
28	8053.5
29	10674.5
30	13666.5
31	17501.0
32	23284.5
33	26567.5
34	27814.0
35	31142.5
36	35663.0
37	39941.5
38	44431.5
39	48334.5
40	52152.0
41	57102.0
42	61155.5
43	66301.0
44	71750.0
45	76906.0
46	82101.0
47	85235.5
48	88060.5
49	91489.0
50	95514.0
51	97165.0
52	94272.0
53	91822.0
54	88503.5
55	82428.5
56	75839.0
57	68128.0
58	61424.5
59	54214.5
60	44454.0
61	35521.0
62	28614.5
63	21538.5
64	15165.5
65	11326.0
66	8602.0
67	6621.5
68	5224.5
69	3923.5
70	2965.5
71	2286.0
72	1847.0
73	1605.0
74	1233.0
75	926.5
76	700.5
77	431.5
78	260.5
79	152.0
80	82.5
81	58.0
82	45.0
83	31.5
84	18.5
85	11.0
86	7.0
87	6.0
88	7.5
89	5.0
90	3.0
91	3.5
92	4.0
93	4.0
94	4.5
95	5.5
96	4.5
97	4.5
98	3.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22411005710005122
2	0.007835013720828907
3	3.821957912599466E-4
4	0.0026753705388196263
5	0.08714064040726784
6	0.05651720263256461
7	0.029620173822645864
8	0.01710326165888261
9	0.014571214541785466
10-11	0.012588573874624493
12-13	0.014642876252646706
14-15	0.023051183660365532
16-17	0.026180411701306344
18-19	0.025535456303555183
20-21	0.01791542771531
22-23	0.015622252967750319
24-25	0.01354406335277436
26-27	0.013066318613699427
28-29	0.015646140204704066
30-31	0.011776407818197106
32-33	0.016004448759010265
34-35	0.01882314271955237
36-37	0.020280264173730918
38-39	0.024579966825405315
40-41	0.025654892488323918
42-43	0.022907860238643052
44-45	0.02071023443889836
46-47	0.021116317467112053
48-49	0.019515872591211025
50-51	0.016291095602455223
52-53	0.01383071019621932
54-55	0.0193486619325348
56-57	0.022812311290828065
58-59	0.01906201508908984
60-61	0.021307415362742024
62-63	0.02773308210329988
64-65	0.01985029390856348
66-67	0.023290056029903
68-69	0.016816614815437652
70-71	0.01774821705663377
72-73	0.026371509596936318
74-75	0.015574478493842825
76-77	0.014045695328803039
78-79	0.017174923369743852
80-81	0.018393172454384932
82-83	0.013735161248404333
84-85	0.017676555345772532
86-87	0.01798708942617124
88-89	0.019420323643396038
90-91	0.014141244276618026
92-93	0.013902371907080558
94-95	0.014762312437415439
96-97	0.015622252967750319
98-99	0.01445177835701673
100-101	0.018894804430413612
102-103	0.02450830511454408
104-105	0.008790503198978773
106-107	0.00881439043593252
108-109	0.015956674285102772
110-111	0.01268412282243948
112-113	0.0103909480748798
114-115	0.007405043455661466
116-117	0.004849109101610573
118-119	0.00300979185617208
120-121	0.0028425811974958533
122-123	0.0018870917193459865
124-125	6.449553977511599E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2093168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.37644609525415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4089996688207	32.88351731335397
2	13.990316219414032	13.256229244492577
3	6.127299768255167	8.708690615406125
4	3.287003932977645	6.229062585824151
5	1.9446622673628997	4.606559354159658
6	1.25684771396991	3.572698678250331
7	0.8467060079056119	2.807974507944764
8	0.5880067034531644	2.228613431183755
9	0.4388588784951573	1.8712416600404558
>10	1.9841603725589796	15.76942871687517
>50	0.08383842731083331	2.725412250247618
>100	0.03946650238365163	3.3672294642604883
>500	0.0022194162112967562	0.7196906247756182
>1k	0.001614120880943092	1.2536515531853125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3292	0.15727356810346804	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2742	0.13099760745434672	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2666	0.12736674743737722	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4332342172247999E-4
2	0.0	0.0	0.0	0.0	1.4332342172247999E-4
3	0.0	0.0	0.0	0.0	1.4332342172247999E-4
4	0.0	0.0	0.0	0.0	1.4332342172247999E-4
5	0.0	0.0	0.0	0.0	1.9109789562997333E-4
6	0.0	0.0	0.0	0.0	2.8664684344495997E-4
7	0.0	0.0	0.0	0.0	2.8664684344495997E-4
8	0.0	0.0	0.0	0.0	2.8664684344495997E-4
9	0.0	0.0	0.0	0.0	2.8664684344495997E-4
10-11	0.0	0.0	0.0	0.0	2.8664684344495997E-4
12-13	0.0	0.0	0.0	2.3887236953746667E-5	7.405043455661467E-4
14-15	0.0	0.0	0.0	4.777447390749333E-5	8.360532933811333E-4
16-17	0.0	0.0	0.0	9.554894781498667E-5	9.554894781498666E-4
18-19	0.0	0.0	0.0	1.1943618476873333E-4	9.554894781498666E-4
20-21	0.0	0.0	0.0	3.3442131735245335E-4	0.0011704746107335864
22-23	0.0	0.0	0.0	0.0010988128998723466	0.0014332342172248
24-25	0.0	0.0	0.0	0.0028186939605421065	0.0014332342172248
26-27	0.0	0.0	0.0	0.0050163197602868005	0.0017915427715309998
28-29	0.0	0.0	0.0	0.021116317467112053	0.00186320448239224
30-31	0.0	0.0	0.0	0.0719722449416387	0.00186320448239224
32-33	0.0	0.0	0.0	0.16023558548573263	0.00186320448239224
34-35	0.0	0.0	0.0	0.3032723603647677	0.0020543023780222134
36-37	0.0	0.0	0.0	0.532924256438088	0.0020543023780222134
38-39	0.0	0.0	0.0	0.8682055143208762	0.00207818961497596
40-41	0.0	0.0	0.0	1.2285683710050985	0.0021020768519297065
42-43	0.0	0.0	0.0	1.5574478493842827	0.0021259640888834533
44-45	0.0	0.0	0.0	1.9603538750831275	0.002197625799744693
46-47	0.0	0.0	0.0	2.461627542557501	0.00229317474755968
48-49	0.0	0.0	0.0	2.969326876772433	0.002484272643189653
50-51	0.0	0.0	0.0	3.493006772509421	0.0025320471170971466
52-53	0.0	0.0	0.0	3.9933727249795523	0.002770919486634613
54-55	0.0	0.0	0.0	4.544714041108979	0.002770919486634613
56-57	0.0	0.0	0.0	5.20548278972352	0.0027948067235883597
58-59	0.0	0.0	0.0	5.908675271167914	0.0028186939605421065
60-61	0.0	0.0	0.0	6.620132736598304	0.002890355671403347
62-63	0.0	0.0	0.0	7.304167654005794	0.0032725514626632934
64-65	0.0	0.0	0.0	8.019924822087859	0.0032964386996170397
66-67	0.0	0.0	0.0	8.84334176712046	0.0032964386996170397
68-69	0.0	0.0	0.0	9.755905880464445	0.0032964386996170397
70-71	0.0	0.0	0.0	10.70807503267774	0.003344213173524533
72-73	0.0	0.0	0.0	11.621594635499875	0.003487536595247013
74-75	0.0	0.0	0.0	12.523505041162487	0.003630860016969493
76-77	0.0	0.0	0.0	13.537398813664264	0.0036786344908769863
78-79	0.0	0.0	0.0	14.638385452099401	0.0037502962017382266
80-81	0.0	0.0	0.0	15.785546119566131	0.0038219579125994664
82-83	0.0	0.0	0.0	16.945343135381393	0.003989168571275693
84-85	0.0	0.0	0.0	18.064770720744825	0.0040369430451831865
86-87	0.0	0.0	0.0	19.23417040581549	0.004299702651674399
88-89	0.0	0.0	0.0	20.4914990101129	0.004538575021211866
90-91	0.0	0.0	0.0	21.80613309586235	0.004562462258165613
92-93	0.0	0.0	0.0	23.11448483829296	0.004968545286379306
94-95	0.0	0.0	0.0	24.387985102008056	0.005040206997240546
96-97	0.0	0.0	0.0	25.68167008094907	0.005494064499361733
98-99	0.0	0.0	0.0	27.053729084335323	0.00587626029062168
100-101	0.0	0.0	0.0	28.501653952286677	0.00587626029062168
102-103	0.0	0.0	0.0	29.95996976831291	0.00587626029062168
104-105	0.0	0.0	0.0	31.345931143606247	0.00587626029062168
106-107	0.0	0.0	0.0	32.735332281020916	0.005924034764529173
108-109	0.0	0.0	0.0	34.162785786902916	0.005924034764529173
110-111	0.0	0.0	0.0	35.63402459812113	0.005971809238436666
112-113	0.0	0.0	0.0	37.12597364377824	0.006067358186251653
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4120	0.0	42.57586	1
GTATCAA	6190	0.0	39.22989	1
TCAACGC	7505	0.0	31.816662	4
CAACGCA	7630	0.0	31.373465	5
ATCAACG	7655	0.0	31.193214	3
AACGCAG	7815	0.0	30.537056	6
TATCAAC	7955	0.0	30.391851	2
GTACATG	7690	0.0	27.77602	1
TACATGG	7690	0.0	27.48991	2
TATACCG	110	7.7175786E-4	27.066885	5
CATGGGG	5025	0.0	26.899902	4
TAATGCG	90	0.009493989	26.465397	5
ACATGGG	7790	0.0	26.295528	3
ACGCAGA	9025	0.0	26.172865	7
CGCAGAG	9205	0.0	25.466547	8
ATGGGGA	2610	0.0	23.955748	5
GCAGAGT	10070	0.0	22.86487	9
CGTACAC	185	2.6754295E-5	22.531351	3
GAGTACT	5470	0.0	22.027037	12-13
AGAGTAC	9065	0.0	21.594664	10-11
>>END_MODULE