FastQCFastQC Report
Mon 15 Aug 2016
SRR2920934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693518
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG46450.034.1081124
GTACATG79700.032.6012421
TACATGG80800.031.2566152
ACATGGG79100.030.8741383
GTATCAA45850.028.0750391
GTATAGT2506.89397E-1026.2215671
ATGGGGC19200.024.817075
TCAACGC50400.024.3443624
AACGCAG51150.024.205266
ATGGGGA22950.023.616775
CAACGCA52400.023.415195
TATCAAC54200.023.1883932
ATCAACG54250.022.9474283
GTGTAGC10350.022.4558851
GTATACG1350.002532340422.0720251
TACGATA1350.00253974922.0609252
ATGGGGG22150.022.049665
GGTATCA35450.022.0222171
TAGTACG1653.193958E-421.6585334
ATAATGG9700.021.4923443
Produced by FastQC (version 0.11.5)