##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920934_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1693518
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.993842994287633	31.0	31.0	34.0	26.0	34.0
2	30.1213928638491	31.0	31.0	34.0	26.0	34.0
3	30.222494239801406	31.0	31.0	34.0	26.0	34.0
4	33.63262687494316	37.0	35.0	37.0	28.0	37.0
5	33.76250798633377	37.0	35.0	37.0	28.0	37.0
6	33.72168999679956	37.0	35.0	37.0	28.0	37.0
7	33.646019705725	37.0	35.0	37.0	28.0	37.0
8	33.5622331737838	37.0	35.0	37.0	28.0	37.0
9	35.021471870980996	39.0	35.0	39.0	27.0	39.0
10-11	35.036577408684174	39.0	35.0	39.0	27.0	39.0
12-13	34.9835357522034	39.0	35.0	39.0	27.0	39.0
14-15	36.16131449444293	40.0	36.0	41.0	27.0	41.0
16-17	36.14757947656889	40.0	36.0	41.0	27.0	41.0
18-19	36.131587027713906	40.0	36.0	41.0	27.0	41.0
20-21	36.153455705814764	40.0	36.0	41.0	27.0	41.0
22-23	36.0693700923167	40.0	36.0	41.0	27.0	41.0
24-25	36.06019392767009	40.0	36.0	41.0	27.0	41.0
26-27	35.97635690910873	40.0	36.0	41.0	27.0	41.0
28-29	35.84943945089453	40.0	36.0	41.0	27.0	41.0
30-31	35.68769685353212	40.0	35.5	41.0	26.5	41.0
32-33	35.54281973973704	40.0	35.0	41.0	26.0	41.0
34-35	35.456288034730065	40.0	35.0	41.0	25.5	41.0
36-37	35.31054526730746	40.0	35.0	41.0	25.0	41.0
38-39	35.1631842708492	39.5	35.0	41.0	24.5	41.0
40-41	35.02770741143584	39.0	35.0	41.0	24.0	41.0
42-43	34.84365031844952	39.0	34.5	41.0	23.5	41.0
44-45	34.69453675721191	39.0	34.0	41.0	23.0	41.0
46-47	34.499085926456054	39.0	34.0	41.0	23.0	41.0
48-49	34.363729821590326	39.0	34.0	41.0	22.0	41.0
50-51	34.162049355247476	38.5	33.0	40.5	20.5	41.0
52-53	33.96148549941601	38.0	33.0	40.0	19.5	41.0
54-55	33.717708934891746	38.0	33.0	40.0	18.5	41.0
56-57	33.45137902283885	38.0	33.0	40.0	17.5	41.0
58-59	33.17601436772446	37.5	32.5	40.0	14.5	41.0
60-61	32.903538373964736	37.0	32.0	40.0	13.0	41.0
62-63	32.58623528064065	36.5	32.0	40.0	10.5	41.0
64-65	32.28323495823487	36.0	31.5	39.0	8.0	41.0
66-67	31.927704340904555	35.5	31.0	39.0	7.5	41.0
68-69	31.587817489982392	35.0	31.0	38.5	7.0	40.0
70-71	31.218977595750385	35.0	31.0	38.0	6.5	40.0
72-73	31.26939188127909	35.0	31.0	37.0	2.0	40.0
74-75	31.250679355046714	35.0	31.0	37.0	2.0	39.0
76-77	30.97601708396368	35.0	31.0	36.5	2.0	39.0
78-79	30.66976849375088	35.0	31.0	36.0	2.0	39.0
80-81	30.38827104288233	35.0	31.0	36.0	2.0	37.0
82-83	30.131298870162585	35.0	31.0	35.0	2.0	37.0
84-85	29.877855151229568	35.0	31.0	35.0	2.0	36.5
86-87	29.66279100665006	35.0	31.0	35.0	2.0	36.0
88-89	29.440825252521673	35.0	30.5	35.0	2.0	36.0
90-91	29.23398836032448	34.5	30.0	35.0	2.0	35.5
92-93	29.026173917253907	34.0	30.0	35.0	2.0	35.0
94-95	28.865891298468632	34.0	30.0	35.0	2.0	35.0
96-97	28.72753433975901	34.0	29.5	35.0	2.0	35.0
98-99	28.54347045617466	34.0	29.0	35.0	2.0	35.0
100-101	28.35711814105312	34.0	29.0	35.0	2.0	35.0
102-103	26.94239889980502	32.5	26.0	34.5	2.0	35.0
104-105	27.917790067776075	34.0	28.5	35.0	2.0	35.0
106-107	28.014817970638635	34.0	29.0	35.0	2.0	35.0
108-109	27.880986207409663	34.0	29.0	35.0	2.0	35.0
110-111	27.708659724904017	34.0	28.0	35.0	2.0	35.0
112-113	27.510127438858046	34.0	27.0	35.0	2.0	35.0
114-115	27.308729520442064	34.0	27.0	35.0	2.0	35.0
116-117	26.987311324709864	34.0	25.0	35.0	2.0	35.0
118-119	26.672608735189115	34.0	25.0	35.0	2.0	35.0
120-121	26.36967720449384	34.0	24.5	35.0	2.0	35.0
122-123	25.947109508136318	34.0	22.0	35.0	2.0	35.0
124-125	24.491165431958798	32.5	10.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53195.0
3	8084.0
4	5579.0
5	6715.0
6	9771.0
7	9507.0
8	7916.0
9	8550.0
10	9086.0
11	9444.0
12	9443.0
13	9328.0
14	9547.0
15	8910.0
16	7425.0
17	7308.0
18	7293.0
19	7611.0
20	8180.0
21	8975.0
22	10849.0
23	13190.0
24	16566.0
25	19828.0
26	22547.0
27	25425.0
28	29367.0
29	34577.0
30	40460.0
31	49845.0
32	62461.0
33	79415.0
34	106929.0
35	153214.0
36	267828.0
37	409891.0
38	139088.0
39	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52988877289383	18.07397593283196	10.798522492362563	25.59761280191165
2	17.09262256642742	21.541755939303872	35.91678439911507	25.448837095153642
3	19.423022433021256	24.796347114076966	29.207352385301615	26.57327806760016
4	13.051731591107599	16.288127203925214	35.84641668297835	34.81372452198884
5	13.625552258158397	38.383102867158144	33.97550750964622	14.015837365037246
6	33.00264655826241	35.86497291667036	17.099414706871652	14.032965818195574
7	27.95406220683711	32.875326820277365	21.199116380826386	17.97149459205913
8	26.826921371956807	34.04653966865458	20.37744770641117	18.74909125297744
9	26.726954823486015	15.133966676203064	18.871249181327894	39.267829318983026
10-11	25.162943455015906	26.14004271872662	27.715872004576838	20.98114182168064
12-13	25.424400385058366	23.904140795680917	27.3786188601776	23.292839959083118
14-15	23.09232628434498	25.27690203019138	24.55875617906814	27.072015506395502
16-17	23.159257642914906	27.85886693954543	25.705699281617562	23.276176135922107
18-19	23.249541884004614	27.1919986148676	26.89541245803681	22.663047043090973
20-21	24.10117279179755	26.575983294452993	26.35756204900577	22.965281864743687
22-23	23.774602267708477	26.391527570285756	26.121749375111335	23.712120786894427
24-25	23.334336814595954	26.59929456941348	26.427560420847634	23.638808195142936
26-27	23.235920410739816	26.61713143372767	26.37039089952599	23.776557256006527
28-29	23.3378467931046	26.970497454568232	26.433579904339865	23.258075847987307
30-31	23.432508895724734	26.96757713665795	26.20294660910691	23.396967358510413
32-33	23.059283761044885	27.196811331709252	26.06083347983519	23.68307142741067
34-35	23.30884182174773	27.04204428681423	26.28271682049203	23.366397070946004
36-37	23.492393195980462	26.678150493282555	26.267982874718935	23.561473436018048
38-39	23.25680131417226	26.853875153635247	26.37849224732911	23.510831284863382
40-41	23.234315085209193	26.929815801121233	26.538472220662822	23.297396893006752
42-43	23.10065833463586	26.899976923865598	26.364350592962104	23.63501414853644
44-45	23.0039503971226	26.826006145850045	26.590684082567467	23.57935937445989
46-47	22.949012174398074	26.855614853191085	26.55651369294802	23.638859279462825
48-49	22.745102209744	26.942026493767685	26.50304363418725	23.80982766230106
50-51	22.625038886083935	27.006780433142968	26.539142961050104	23.829037719722994
52-53	22.92787613286268	26.743447447816525	26.454212198814226	23.87446422050657
54-55	22.530533283265612	26.707357030019065	26.614706142168888	24.147403544546435
56-57	22.594168467224627	26.74674311491553	26.427266568358615	24.231821849501227
58-59	22.643020507084927	26.311743849617468	26.643287561808105	24.401948081489504
60-61	22.45596837085188	26.408341472840046	26.74506001372046	24.390630142587614
62-63	22.428545894740264	26.23044050501275	26.858087397872588	24.482926202374394
64-65	22.273007110015584	26.12469882578985	26.765066982089664	24.837227082104903
66-67	22.267317831774992	26.28512393398105	26.698370147244816	24.74918808699914
68-69	22.683529374922077	25.894270488350067	26.85269493911947	24.569505197608386
70-71	22.306361771174764	25.745786170396617	27.049248371853942	24.89860368657468
72-73	22.277089815749463	25.725329006159015	27.099815397922494	24.897765780169028
74-75	22.147464634573257	25.710521223687337	27.168624068135593	24.97339007360381
76-77	22.03774666363338	25.506770231662173	27.433516413712418	25.02196669099203
78-79	22.19787225682524	25.670588297855684	27.45400825607603	24.677531189243055
80-81	22.21762016826186	25.498750059823415	27.32323139816636	24.96039837374836
82-83	22.19144734023774	25.416635276287042	27.371199732923262	25.020717650551955
84-85	22.11784191272707	25.3593040288495	27.331834842165037	25.191019216258393
86-87	22.061098718131653	25.22507511859003	27.43628398505043	25.277542178227886
88-89	22.17357934503756	25.05403268785591	27.37215021146308	25.400237755643445
90-91	22.168053945065253	25.117100083458276	27.39384181333412	25.32100415814235
92-93	22.369594155067258	24.926072779244013	27.370737405194433	25.333595660494296
94-95	22.110899574444954	24.802230943678065	27.583997192116655	25.502872289760326
96-97	22.165003270076408	24.556294645995767	27.54854748936708	25.730154594560744
98-99	22.183376934583947	24.71425819975281	27.420253430792172	25.682111434871075
100-101	22.03088228039584	24.857125217890136	27.576021908795862	25.53597059291816
102-103	21.87522338716681	24.907381095995778	27.59464378866998	25.62275172816743
104-105	21.59382934705167	25.045429787053042	27.502360104021804	25.858380761873477
106-107	21.603011129551717	25.136438484367403	27.446306426921733	25.81424395915915
108-109	21.57298976058601	25.23815699410675	27.51548939943995	25.673363845867293
110-111	21.553469766397523	25.587167374239616	27.45329870805156	25.4060641513113
112-113	21.488006906121825	25.90755853407992	27.34884512425022	25.255589435548032
114-115	21.326902060471337	26.042075674998543	27.280320240044126	25.350702024485987
116-117	21.145973951994876	25.99505938225437	27.34095078380085	25.518015881949907
118-119	21.338195423281604	26.02097537038082	27.15101835337522	25.489810852962353
120-121	21.3641740634864	26.24276616438016	27.197153854244345	25.195905917889093
122-123	21.444052109626856	26.518938427068267	26.955145022074745	25.081864441230135
124-125	21.48043618992409	26.63317859800992	26.707133632285405	25.179251579780583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	874.0
1	694.0
2	440.0
3	341.0
4	277.5
5	224.5
6	201.5
7	203.5
8	218.0
9	252.0
10	253.5
11	222.5
12	260.0
13	315.5
14	351.5
15	400.5
16	471.5
17	554.5
18	649.0
19	799.5
20	969.0
21	1242.0
22	1584.5
23	2017.0
24	2607.5
25	3318.5
26	4417.5
27	6016.5
28	7513.5
29	9226.5
30	11359.0
31	14115.0
32	18582.5
33	21611.0
34	23483.5
35	26755.0
36	30931.5
37	34749.5
38	38168.0
39	41621.5
40	45273.0
41	49369.0
42	52425.0
43	56985.5
44	61950.0
45	65616.5
46	69358.5
47	71770.5
48	73706.0
49	76176.5
50	78698.5
51	78776.5
52	75251.5
53	71453.5
54	67592.5
55	61980.5
56	55732.0
57	49584.5
58	44640.0
59	38946.5
60	31767.0
61	26078.5
62	20866.5
63	15409.5
64	11076.0
65	8298.5
66	6307.5
67	4836.0
68	3684.5
69	2794.5
70	2222.5
71	1682.5
72	1331.5
73	1083.0
74	816.0
75	598.5
76	426.5
77	299.5
78	200.5
79	127.0
80	84.5
81	68.0
82	50.0
83	40.0
84	37.0
85	35.0
86	27.5
87	17.0
88	12.5
89	10.5
90	7.5
91	9.0
92	8.0
93	6.0
94	6.5
95	5.5
96	7.0
97	7.0
98	4.5
99	6.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16728490633108123
2	0.14980649748039288
3	0.17826796054131103
4	0.14768074505260648
5	0.17472503982833368
6	0.1114248564231381
7	0.10859051985275621
8	0.09613124867878582
9	0.10357138217603828
10-11	0.08975399139542656
12-13	0.07717662286435692
14-15	0.07753091493565466
16-17	0.07756043927492946
18-19	0.07446038365107428
20-21	0.06766978561786766
22-23	0.06002298174569152
24-25	0.06356590245866889
26-27	0.07407656724050173
28-29	0.06923457559943266
30-31	0.06684310411817294
32-33	0.0753165894900438
34-35	0.0726889232945856
36-37	0.07575945457916597
38-39	0.07074031690244804
40-41	0.0750508704365705
42-43	0.07637946570393701
44-45	0.0682897967426387
46-47	0.06578022790427973
48-49	0.05721816951458444
50-51	0.06344780510156962
52-53	0.06637071468977596
54-55	0.0675221639214936
56-57	0.0719508148127153
58-59	0.05792675365717991
60-61	0.06820122372481427
62-63	0.06409734056561547
64-65	0.05763151026443179
66-67	0.06285731831607341
68-69	0.06524878979733312
70-71	0.06055441985263812
72-73	0.0565981583898134
74-75	0.05966868967439378
76-77	0.06985458672420371
78-79	0.06303446435172227
80-81	0.06123347965595878
82-83	0.06616404431485227
84-85	0.06784693165351653
86-87	0.06067251720973736
88-89	0.06046584683481369
90-91	0.0623258802091268
92-93	0.057572461585882174
94-95	0.06731549354656992
96-97	0.0529666646590116
98-99	0.0534981027659582
100-101	0.051047582606148854
102-103	0.04895135451763725
104-105	0.0470027481254997
106-107	0.05887153251397387
108-109	0.0584286674248517
110-111	0.0657211792257301
112-113	0.06533736281515756
114-115	0.06864408881393644
116-117	0.07200986349126494
118-119	0.06521926545805831
120-121	0.06637071468977596
122-123	0.0641268649048903
124-125	0.07519849213294456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1693518.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.07161605627291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.45605510622576	37.20832266387267
2	14.019918904893352	14.600796687113773
3	5.808100702306484	9.073115693600167
4	2.9031809587964745	6.046932969133291
5	1.6235859611726082	4.227137240226743
6	1.0260914010228963	3.2058142483624446
7	0.7118260337443106	2.594615234959531
8	0.45655588338169	1.9018882134147097
9	0.3350927982515478	1.570394118039904
>10	1.5663662832225047	13.880282781186631
>50	0.06491506860736218	2.289260105788628
>100	0.025923528702047782	2.4111294721369947
>500	0.0018189483222728888	0.6325816709994893
>1k	5.684213507102778E-4	0.35772890116506767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.952433927481137E-5	0.0
18-19	0.0	0.0	0.0	8.857301782443412E-5	0.0
20-21	0.0	0.0	0.0	1.771460356488682E-4	0.0
22-23	0.0	0.0	0.0	3.838164105725478E-4	0.0
24-25	0.0	0.0	0.0	0.0011809735709924548	0.0
26-27	0.0	0.0	0.0	0.0028933852489315143	0.0
28-29	0.0	0.0	0.0	0.01836413902893267	0.0
30-31	0.0	0.0	0.0	0.06784693165351653	0.0
32-33	0.0	0.0	0.0	0.15550469496043148	0.0
34-35	0.0	0.0	0.0	0.29604054990853357	0.0
36-37	0.0	0.0	0.0	0.5281904296263754	2.952433927481137E-5
38-39	0.0	0.0	0.0	0.8695803646610193	5.904867854962274E-5
40-41	0.0	0.0	0.0	1.2533672508942924	5.904867854962274E-5
42-43	0.0	0.0	0.0	1.6035849633721049	5.904867854962274E-5
44-45	0.0	0.0	0.0	2.0229191540922504	8.857301782443412E-5
46-47	0.0	0.0	0.0	2.5299406324585862	1.1809735709924548E-4
48-49	0.0	0.0	0.0	3.056654845121221	1.1809735709924548E-4
50-51	0.0	0.0	0.0	3.622429758644431	1.1809735709924548E-4
52-53	0.0	0.0	0.0	4.161544193802486	1.1809735709924548E-4
54-55	0.0	0.0	0.0	4.7489604480141345	1.1809735709924548E-4
56-57	0.0	0.0	0.0	5.421849664426359	1.4762169637405685E-4
58-59	0.0	0.0	0.0	6.145343598355613	1.771460356488682E-4
60-61	0.0	0.0	0.0	6.896678984221012	1.771460356488682E-4
62-63	0.0	0.0	0.0	7.628469257486486	1.771460356488682E-4
64-65	0.0	0.0	0.0	8.378210329031045	1.771460356488682E-4
66-67	0.0	0.0	0.0	9.252573636654585	1.771460356488682E-4
68-69	0.0	0.0	0.0	10.208601266712252	1.771460356488682E-4
70-71	0.0	0.0	0.0	11.233568228976605	1.771460356488682E-4
72-73	5.904867854962274E-5	0.0	0.0	12.219858306790952	1.771460356488682E-4
74-75	5.904867854962274E-5	2.952433927481137E-5	0.0	13.177864067580032	1.771460356488682E-4
76-77	5.904867854962274E-5	5.904867854962274E-5	0.0	14.2432498503116	1.771460356488682E-4
78-79	5.904867854962274E-5	5.904867854962274E-5	0.0	15.413299415772375	1.771460356488682E-4
80-81	5.904867854962274E-5	8.857301782443412E-5	0.0	16.625244018664105	1.771460356488682E-4
82-83	5.904867854962274E-5	1.1809735709924548E-4	0.0	17.843270635446448	1.771460356488682E-4
84-85	5.904867854962274E-5	1.4762169637405685E-4	0.0	19.002219049339892	1.771460356488682E-4
86-87	5.904867854962274E-5	1.771460356488682E-4	0.0	20.204656814985135	1.771460356488682E-4
88-89	5.904867854962274E-5	1.771460356488682E-4	0.0	21.50626683625447	1.771460356488682E-4
90-91	5.904867854962274E-5	1.771460356488682E-4	0.0	22.862408312164384	1.771460356488682E-4
92-93	5.904867854962274E-5	1.771460356488682E-4	0.0	24.226344213642843	1.771460356488682E-4
94-95	5.904867854962274E-5	1.771460356488682E-4	0.0	25.541210663246567	1.771460356488682E-4
96-97	5.904867854962274E-5	1.771460356488682E-4	0.0	26.87405743546865	1.771460356488682E-4
98-99	5.904867854962274E-5	1.771460356488682E-4	0.0	28.27498733405845	1.771460356488682E-4
100-101	5.904867854962274E-5	2.3619471419849097E-4	0.0	29.753271001548256	1.771460356488682E-4
102-103	5.904867854962274E-5	2.3619471419849097E-4	0.0	31.260665667563025	1.771460356488682E-4
104-105	5.904867854962274E-5	2.3619471419849097E-4	0.0	32.678158720485996	1.771460356488682E-4
106-107	5.904867854962274E-5	2.3619471419849097E-4	0.0	34.066747445258926	1.771460356488682E-4
108-109	5.904867854962274E-5	2.3619471419849097E-4	0.0	35.49667024501659	1.771460356488682E-4
110-111	5.904867854962274E-5	2.3619471419849097E-4	0.0	36.98829891385861	1.771460356488682E-4
112-113	5.904867854962274E-5	2.3619471419849097E-4	0.0	38.487338191858605	1.771460356488682E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	4645	0.0	34.108112	4
GTACATG	7970	0.0	32.601242	1
TACATGG	8080	0.0	31.256615	2
ACATGGG	7910	0.0	30.874138	3
GTATCAA	4585	0.0	28.075039	1
GTATAGT	250	6.89397E-10	26.221567	1
ATGGGGC	1920	0.0	24.81707	5
TCAACGC	5040	0.0	24.344362	4
AACGCAG	5115	0.0	24.20526	6
ATGGGGA	2295	0.0	23.61677	5
CAACGCA	5240	0.0	23.41519	5
TATCAAC	5420	0.0	23.188393	2
ATCAACG	5425	0.0	22.947428	3
GTGTAGC	1035	0.0	22.455885	1
GTATACG	135	0.0025323404	22.072025	1
TACGATA	135	0.002539749	22.060925	2
ATGGGGG	2215	0.0	22.04966	5
GGTATCA	3545	0.0	22.022217	1
TAGTACG	165	3.193958E-4	21.658533	4
ATAATGG	970	0.0	21.492344	3
>>END_MODULE