FastQCFastQC Report
Mon 15 Aug 2016
SRR2920934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693518
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29710.17543362397092915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.14089014701939984No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT18670.11024388285214566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.10020560749870978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57050.036.2869341
GGTATCA40250.035.2766651
CATGGGG44000.031.8012164
TACATGG76600.029.304962
TCAACGC70100.029.2192444
TATCAAC70800.029.1826522
GTACATG77900.029.0253981
CAACGCA70650.028.9074975
ACATGGG76250.028.8147643
AACGCAG71600.028.59196
ATCAACG72400.028.455493
TATACCG1807.248127E-726.4634235
ATGGGGA23250.026.3780575
ACGCAGA84050.024.3523757
CGCAGAG85800.023.8535658
GTATAAT8100.023.5690141
AGTACTT51850.021.97494512-13
GCAGAGT91550.021.9004849
GAGTACT48450.021.85918812-13
ATGGGGG21100.021.4466615
Produced by FastQC (version 0.11.5)