##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920934_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1693518 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.243656695706807 31.0 31.0 34.0 28.0 34.0 2 31.335943875412013 33.0 31.0 34.0 28.0 34.0 3 31.423728002891025 33.0 31.0 34.0 28.0 34.0 4 34.98374153684815 37.0 35.0 37.0 32.0 37.0 5 34.71937351714006 37.0 35.0 37.0 32.0 37.0 6 34.45298662311236 37.0 35.0 37.0 30.0 37.0 7 34.35922027400949 37.0 35.0 37.0 30.0 37.0 8 34.34811498903466 37.0 35.0 37.0 30.0 37.0 9 35.91378715785719 39.0 37.0 39.0 30.0 39.0 10-11 35.8972535278633 39.0 36.0 39.0 30.0 39.0 12-13 35.79706947313226 39.0 35.0 39.0 30.0 39.0 14-15 37.027816946734546 40.0 37.0 41.0 31.0 41.0 16-17 37.03938635432277 40.0 37.0 41.0 31.0 41.0 18-19 37.08068765729092 40.0 37.0 41.0 31.0 41.0 20-21 37.07442141152323 40.0 37.0 41.0 30.0 41.0 22-23 37.02138949807442 40.0 37.0 41.0 30.0 41.0 24-25 36.99749338359557 40.0 37.0 41.0 30.0 41.0 26-27 36.867008204223396 40.0 37.0 41.0 30.0 41.0 28-29 36.766938703928744 40.0 36.5 41.0 30.0 41.0 30-31 36.61269381252517 40.0 36.0 41.0 30.0 41.0 32-33 36.454851675624354 40.0 36.0 41.0 30.0 41.0 34-35 36.32898469340155 40.0 36.0 41.0 29.0 41.0 36-37 36.185290324637826 40.0 35.5 41.0 29.0 41.0 38-39 36.05160529737505 40.0 35.0 41.0 28.0 41.0 40-41 35.90465321301575 40.0 35.0 41.0 27.5 41.0 42-43 35.746875143931156 40.0 35.0 41.0 27.0 41.0 44-45 35.54970776808986 40.0 35.0 41.0 26.5 41.0 46-47 35.332457641430445 39.0 35.0 41.0 25.5 41.0 48-49 35.07833988183178 39.0 35.0 41.0 24.0 41.0 50-51 34.8958366548215 39.0 35.0 41.0 24.0 41.0 52-53 34.700761964148 39.0 34.0 41.0 23.5 41.0 54-55 34.50492672649479 39.0 34.0 41.0 23.0 41.0 56-57 34.25906101972344 38.0 34.0 41.0 22.0 41.0 58-59 34.028817526592576 38.0 34.0 40.0 22.0 41.0 60-61 33.76510051856549 37.5 33.0 40.0 21.5 41.0 62-63 33.46517190841786 37.0 33.0 40.0 20.0 41.0 64-65 33.14953487355906 36.5 33.0 40.0 19.5 41.0 66-67 32.81767391902537 36.0 32.5 39.0 18.5 41.0 68-69 32.4328403949648 35.5 32.0 39.0 17.5 41.0 70-71 32.06964142099464 35.0 32.0 38.5 14.0 40.0 72-73 31.694392678436248 35.0 32.0 37.0 12.5 40.0 74-75 31.356070912739042 35.0 31.0 37.0 10.5 39.0 76-77 31.134061167345138 35.0 31.5 36.5 8.0 39.0 78-79 30.979268599448012 35.0 31.5 36.0 7.5 38.5 80-81 30.722517564029435 35.0 31.0 36.0 7.0 37.0 82-83 30.4949841100006 35.0 31.0 35.0 6.0 37.0 84-85 30.249240929237246 35.0 31.0 35.0 2.0 36.5 86-87 30.081466804604382 35.0 31.0 35.0 2.0 36.0 88-89 29.921258291910686 35.0 31.0 35.0 2.0 36.0 90-91 29.750351930124154 35.0 31.0 35.0 2.0 35.5 92-93 29.623755991964657 35.0 31.0 35.0 2.0 35.0 94-95 29.524041374228084 34.5 31.0 35.0 2.0 35.0 96-97 29.45824786037113 34.0 30.5 35.0 2.0 35.0 98-99 29.34139200173839 34.0 30.0 35.0 2.0 35.0 100-101 29.23137486581188 34.0 30.0 35.0 2.0 35.0 102-103 27.77022771532396 32.5 27.5 34.0 2.0 35.0 104-105 28.84969424594247 34.0 29.5 35.0 2.0 35.0 106-107 29.019458901529244 34.0 30.0 35.0 2.0 35.0 108-109 29.096569685117018 34.5 30.0 35.0 2.0 35.0 110-111 29.237537776392102 35.0 31.0 35.0 2.0 35.0 112-113 29.158587626467508 35.0 31.0 35.0 2.0 35.0 114-115 29.007155518866647 35.0 31.0 35.0 2.0 35.0 116-117 28.819262328478352 35.0 30.0 35.0 2.0 35.0 118-119 28.645013811485914 35.0 30.0 35.0 2.0 35.0 120-121 28.37692247735188 35.0 30.0 35.0 2.0 35.0 122-123 28.093162576364705 35.0 29.5 35.0 2.0 35.0 124-125 27.256196863570388 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25995.0 3 11059.0 4 4639.0 5 4146.0 6 5198.0 7 6239.0 8 6801.0 9 7542.0 10 8971.0 11 10336.0 12 10696.0 13 12201.0 14 14571.0 15 12729.0 16 7992.0 17 7091.0 18 7031.0 19 7152.0 20 7290.0 21 7547.0 22 7872.0 23 8387.0 24 10677.0 25 13815.0 26 17763.0 27 21502.0 28 25685.0 29 31465.0 30 38012.0 31 46542.0 32 58514.0 33 74003.0 34 99584.0 35 144464.0 36 265729.0 37 464929.0 38 179011.0 39 338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.412407180213755 18.573544755604402 11.210221465479947 25.8038265987019 2 17.212988902694253 21.598054546399396 35.4852768184889 25.703679732417456 3 19.489073895505967 24.599102575533816 29.896729461185124 26.01509406777509 4 13.47618282782713 16.215269822448867 35.62496014134175 34.68358720838224 5 13.457096996860699 37.968294943076515 34.48385907182571 14.090748988237072 6 33.132345052332575 35.85868432433199 16.782757781354054 14.226212841981384 7 27.904689734277333 32.325742241511186 21.41722693716367 18.35234108704781 8 27.262977427659074 33.266755922131594 20.64393861098102 18.826328039228308 9 26.995072960694124 13.833151539382357 19.37177443691612 39.8000010630074 10-11 25.43440773855221 25.27918197172452 28.130765233207743 21.155645056515525 12-13 25.974905296179575 22.936853391192553 27.96928847157232 23.118952841055556 14-15 23.328329926035604 24.301646694871984 25.37802400749596 26.991999371596453 16-17 23.111696873354575 27.087090527927245 26.51805919237063 23.283153406347555 18-19 23.069928804026357 26.07442901325257 28.244564031341703 22.61107815137937 20-21 23.813811118086484 25.441707382036654 27.921274890610352 22.823206609266506 22-23 23.48845628245877 25.391217868992065 27.735985665876495 23.384340182672673 24-25 23.209609262725483 25.601442605950602 28.01193746384701 23.177010667476907 26-27 23.005175870034257 25.655354791732428 28.03385147151618 23.30561786671714 28-29 23.195587552238955 25.81039218969108 28.033033711382004 22.960986546687963 30-31 23.263681891717685 25.77797226284657 27.86090144419927 23.097444401236476 32-33 22.986546017945532 25.967605676639653 27.682491574836092 23.363356730578715 34-35 23.24386016199664 25.75315444604886 27.90656402351697 23.09642136843753 36-37 23.330055574257486 25.52579980274387 27.80331083196022 23.340833791038428 38-39 23.16139611786555 25.594559070239104 27.9248378703453 23.31920694155005 40-41 23.168214380002162 25.648926286793262 28.025157945902972 23.157701387301604 42-43 23.156116090056287 25.539936409802706 27.76101614383067 23.54293135631034 44-45 23.007544507693485 25.589786551469878 27.878386634684183 23.524282306152454 46-47 23.130778198672587 25.606950788380466 27.636902040965783 23.62536897198116 48-49 22.91801024783314 25.82563800911391 27.376750789612313 23.87960095344063 50-51 22.803388669395083 25.957573717437647 27.406911987905204 23.832125625262062 52-53 22.984056230164775 25.80164717248776 27.334016013455177 23.880280583892294 54-55 22.537989522982702 25.824459760339707 27.380598028619858 24.256952688057737 56-57 22.582165488855757 26.028749984865783 27.02264698421642 24.366437542062037 58-59 22.405785110056527 25.712840474509424 27.390331042168476 24.49104337326557 60-61 22.408101518207836 25.955557287961618 27.27181185361444 24.364529340216105 62-63 22.237644112644112 25.931475618975618 27.30408949158949 24.526790776790776 64-65 21.92125870784717 25.87016459975721 27.262445677001068 24.946131015394553 66-67 22.054595049361392 26.023882344151566 27.102860867051287 24.81866173943575 68-69 22.51137882342936 25.67831488882209 27.09837197339833 24.71193431435022 70-71 22.196476455419155 25.542376229613 27.156298196415037 25.104849118552806 72-73 22.3317167064991 25.643444156385687 26.952495504639472 25.07234363247574 74-75 22.28771916977181 25.67366496121316 26.859873967507703 25.17874190150733 76-77 22.184652962203767 25.643987510903155 26.978144080523613 25.193215446369464 78-79 22.33343757003865 25.850407630481175 26.784020070142738 25.032134729337436 80-81 22.233564563546572 25.7933503497982 26.619920602954533 25.35316448370069 82-83 22.217957106850193 25.813547791208297 26.594526505264206 25.373968596677305 84-85 22.18564254327231 25.639306910724553 26.525999115906334 25.649051430096804 86-87 22.090060401505387 25.677357629768743 26.51524211539626 25.717339853329612 88-89 22.149888689500845 25.598550468289577 26.389107487347456 25.862453354862126 90-91 22.107119677840174 25.52026461610055 26.359532539902357 26.01308316615692 92-93 22.14982376436482 25.486356908091423 26.367520320173952 25.9962990073698 94-95 21.945884336516926 25.36848523682062 26.53161619129313 26.154014235369328 96-97 22.039921076767076 25.12022446230447 26.55373470950947 26.286119751418983 98-99 22.10884564653733 25.258271524447913 26.298119468536598 26.33476336047816 100-101 22.04908813499163 25.237374875277606 26.42747527624958 26.28606171348118 102-103 21.835941041376344 25.256894900550954 26.438683793288675 26.468480264784027 104-105 21.77326156471724 25.506007127651092 26.2578355443618 26.462895763269863 106-107 21.772463159187684 25.768003493562215 26.0949196601749 26.364613687075206 108-109 21.65177556394552 25.965205076735092 26.089607646166375 26.29341171315301 110-111 21.62604036223075 26.139515354413362 26.047832671574323 26.18661161178156 112-113 21.396136308407947 26.48796298292085 25.949542940880683 26.166357767790522 114-115 21.34960413009748 26.651322986437233 25.854652338680985 26.144420544784303 116-117 21.24320618476343 26.682893875940977 25.904070502145913 26.169829437149676 118-119 21.315474334627705 26.908162325330913 25.665401821565904 26.110961518475477 120-121 21.3155242143826 27.089975240289654 25.59559415333069 25.998906391997057 122-123 21.16113024148559 27.297694444600268 25.405012646911285 26.136162667002854 124-125 21.021445978207403 27.510774952155607 25.380644117457756 26.08713495217923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 40.0 2 100.5 3 192.5 4 151.0 5 97.0 6 178.0 7 348.0 8 547.5 9 609.5 10 483.5 11 378.5 12 384.0 13 407.0 14 464.5 15 512.0 16 538.0 17 623.0 18 710.0 19 775.5 20 920.5 21 1111.0 22 1348.5 23 1760.5 24 2344.0 25 2987.0 26 4007.0 27 5605.5 28 7035.5 29 8889.0 30 11014.0 31 13905.0 32 18818.0 33 21889.5 34 23503.5 35 26713.5 36 31014.5 37 34662.0 38 37878.5 39 41132.5 40 44703.0 41 48670.0 42 51890.5 43 56513.0 44 60952.5 45 64508.0 46 68655.0 47 71630.0 48 73447.5 49 75754.5 50 79382.0 51 79917.5 52 75899.5 53 72288.5 54 68918.5 55 63425.0 56 57212.5 57 50982.5 58 45598.5 59 39887.5 60 32645.0 61 26442.5 62 20909.0 63 15267.5 64 10925.0 65 8128.0 66 6114.5 67 4695.5 68 3462.0 69 2614.0 70 2102.5 71 1576.0 72 1179.5 73 950.5 74 712.0 75 495.0 76 364.5 77 223.0 78 118.0 79 72.0 80 50.0 81 31.5 82 21.0 83 16.5 84 10.0 85 9.0 86 10.5 87 7.0 88 3.0 89 3.0 90 4.5 91 3.5 92 2.0 93 2.5 94 3.0 95 5.0 96 4.5 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22550690338100926 2 0.007912522925649448 3 2.952433927481137E-4 4 0.0020076550706871732 5 0.08396722089756353 6 0.052080934480767255 7 0.025804272526185137 8 0.014821218315955306 9 0.012341173816871151 10-11 0.010510664781832848 12-13 0.012547844191794832 14-15 0.019987977689047295 16-17 0.022290876152482583 18-19 0.022113730116833714 20-21 0.015293607744352289 22-23 0.012754514566718512 24-25 0.011425919299352 26-27 0.010599237799657282 28-29 0.012843087584542945 30-31 0.00930016687156558 32-33 0.013433574370039173 34-35 0.016120289244047008 36-37 0.01700601942229135 38-39 0.020755610510192393 40-41 0.022143254456108527 42-43 0.01963368561774956 44-45 0.01768507922561201 46-47 0.01771460356488682 48-49 0.01665172735099361 50-51 0.013463098709313984 52-53 0.011337346281527567 54-55 0.01700601942229135 56-57 0.01975178297484881 58-59 0.016208862261871442 60-61 0.017980322618360123 62-63 0.02350137406274985 64-65 0.017094592440115783 66-67 0.02022417240324579 68-69 0.014230731530459081 70-71 0.014850742655230119 72-73 0.022645168223780317 74-75 0.013256428334390306 76-77 0.01186878438847417 78-79 0.015145986047978233 80-81 0.015116461708703422 82-83 0.01145544363862681 84-85 0.01526408340507748 86-87 0.015677424154924836 88-89 0.0166222030117188 90-91 0.01163258967427568 92-93 0.011130675906603885 94-95 0.011839260049199359 96-97 0.01340405003076436 98-99 0.011425919299352 100-101 0.015205034726527856 102-103 0.02152324333133749 104-105 0.006672500676107369 106-107 0.006938219729580672 108-109 0.013285952673665115 110-111 0.010481140442558035 112-113 0.008119193300573127 114-115 0.005639148801488971 116-117 0.0038972127842751008 118-119 0.0017714603564886822 120-121 0.0024209958205345323 122-123 0.0012695465888168888 124-125 4.133407498473591E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1693518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.68280173970422 #Duplication Level Percentage of deduplicated Percentage of total 1 70.78655988832558 35.87661180655705 2 14.274685332182607 14.469620931753498 3 5.853234039885816 8.899749011388625 4 2.9939131316321563 6.069596227056382 5 1.7050716652690712 4.320890458140983 6 1.0613562548079516 3.2275505182575848 7 0.7097075163463586 2.517897574091227 8 0.4903840831145289 1.9883231408640245 9 0.36355231740850646 1.6583265022702816 >10 1.657989096923614 14.310428257333987 >50 0.0704380121565273 2.40991956364519 >100 0.03007798856681013 2.692547077846753 >500 0.0018650297726395958 0.6002924102877765 >1k 0.0011656436078997475 0.9582465205066169 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2971 0.17543362397092915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.14089014701939984 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 1867 0.11024388285214566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1697 0.10020560749870978 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.952433927481137E-5 0.0 18-19 0.0 0.0 0.0 8.857301782443412E-5 0.0 20-21 0.0 0.0 0.0 1.771460356488682E-4 0.0 22-23 0.0 0.0 0.0 3.838164105725478E-4 0.0 24-25 0.0 0.0 0.0 0.0011219248924428321 0.0 26-27 0.0 0.0 0.0 0.0028638609096567026 0.0 28-29 0.0 0.0 0.0 0.018393663368207483 0.0 30-31 0.0 0.0 0.0 0.0680240776891654 0.0 32-33 0.0 0.0 0.0 0.15470753780001156 0.0 34-35 0.0 0.0 0.0 0.29453480860551823 0.0 36-37 0.0 0.0 0.0 0.5265075422877111 0.0 38-39 0.0 0.0 0.0 0.8679565260009046 0.0 40-41 0.0 0.0 0.0 1.2502376709311622 0.0 42-43 0.0 0.0 0.0 1.5987429717310357 0.0 44-45 0.0 0.0 0.0 2.0160695073804944 0.0 46-47 0.0 0.0 0.0 2.5205518925691965 0.0 48-49 0.0 0.0 0.0 3.0472956295711056 5.904867854962274E-5 50-51 0.0 0.0 0.0 3.6132772134692397 5.904867854962274E-5 52-53 0.0 0.0 0.0 4.152332599948746 5.904867854962274E-5 54-55 0.0 0.0 0.0 4.739453610767645 5.904867854962274E-5 56-57 0.0 0.0 0.0 5.415236212428802 5.904867854962274E-5 58-59 0.0 0.0 0.0 6.143158797249276 5.904867854962274E-5 60-61 0.0 0.0 0.0 6.896767557238837 5.904867854962274E-5 62-63 0.0 0.0 0.0 7.630742631610647 5.904867854962274E-5 64-65 0.0 0.0 0.0 8.380985616922878 5.904867854962274E-5 66-67 0.0 0.0 0.0 9.255614643599891 5.904867854962274E-5 68-69 0.0 0.0 0.0 10.211878468371756 5.904867854962274E-5 70-71 0.0 0.0 0.0 11.233450131619504 5.904867854962274E-5 72-73 2.952433927481137E-5 0.0 0.0 12.214071536293089 5.904867854962274E-5 74-75 5.904867854962274E-5 0.0 0.0 13.166556245637778 5.904867854962274E-5 76-77 5.904867854962274E-5 0.0 0.0 14.228605778031294 5.904867854962274E-5 78-79 5.904867854962274E-5 0.0 0.0 15.397474369921076 5.904867854962274E-5 80-81 5.904867854962274E-5 0.0 0.0 16.606702733599526 5.904867854962274E-5 82-83 5.904867854962274E-5 0.0 0.0 17.822692170971905 5.904867854962274E-5 84-85 5.904867854962274E-5 0.0 0.0 18.97862910225932 5.904867854962274E-5 86-87 5.904867854962274E-5 0.0 0.0 20.182041171100632 8.857301782443412E-5 88-89 5.904867854962274E-5 0.0 0.0 21.482470218798973 1.4762169637405685E-4 90-91 5.904867854962274E-5 0.0 0.0 22.836781185673846 1.771460356488682E-4 92-93 5.904867854962274E-5 0.0 0.0 24.199240870188568 2.3619471419849097E-4 94-95 5.904867854962274E-5 0.0 0.0 25.512070140382328 2.3619471419849097E-4 96-97 5.904867854962274E-5 0.0 0.0 26.847160762389297 2.3619471419849097E-4 98-99 5.904867854962274E-5 0.0 0.0 28.249744023978486 2.3619471419849097E-4 100-101 5.904867854962274E-5 0.0 0.0 29.735084008554974 2.3619471419849097E-4 102-103 5.904867854962274E-5 0.0 0.0 31.246582557728942 2.3619471419849097E-4 104-105 5.904867854962274E-5 0.0 0.0 32.67074811132802 2.3619471419849097E-4 106-107 8.857301782443412E-5 0.0 0.0 34.06556647168793 2.3619471419849097E-4 108-109 1.1809735709924548E-4 0.0 0.0 35.50945428392258 2.3619471419849097E-4 110-111 1.1809735709924548E-4 0.0 0.0 37.01552035466998 2.3619471419849097E-4 112-113 1.1809735709924548E-4 0.0 0.0 38.5308275436104 2.3619471419849097E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5705 0.0 36.286934 1 GGTATCA 4025 0.0 35.276665 1 CATGGGG 4400 0.0 31.801216 4 TACATGG 7660 0.0 29.30496 2 TCAACGC 7010 0.0 29.219244 4 TATCAAC 7080 0.0 29.182652 2 GTACATG 7790 0.0 29.025398 1 CAACGCA 7065 0.0 28.907497 5 ACATGGG 7625 0.0 28.814764 3 AACGCAG 7160 0.0 28.5919 6 ATCAACG 7240 0.0 28.45549 3 TATACCG 180 7.248127E-7 26.463423 5 ATGGGGA 2325 0.0 26.378057 5 ACGCAGA 8405 0.0 24.352375 7 CGCAGAG 8580 0.0 23.853565 8 GTATAAT 810 0.0 23.569014 1 AGTACTT 5185 0.0 21.974945 12-13 GCAGAGT 9155 0.0 21.900484 9 GAGTACT 4845 0.0 21.859188 12-13 ATGGGGG 2110 0.0 21.446661 5 >>END_MODULE