FastQCFastQC Report
Mon 15 Aug 2016
SRR2920933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549407
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70900.037.1426241
GGTATCA46900.031.6317351
TCAACGC79400.031.5763554
CAACGCA81200.030.9497265
AACGCAG82650.030.5314736
ATCAACG82750.030.44243
TATCAAC85250.030.318522
CATGGGG85000.029.7062154
GTACATG134400.029.4794181
ACATGGG129300.028.7403873
TACATGG136000.027.8503632
ACGCAGA97650.025.8400827
CGCAGAG98050.025.4911678
GCGTTAG2052.2392069E-623.2505191
GCAGAGT110600.021.1455659
ATGGGGA51150.020.8404835
AGAGTAC97350.019.1617210-11
ATGGGGC34050.018.7140185
GTATATA15450.018.1244951
CAGAGTA103500.017.5344610-11
Produced by FastQC (version 0.11.5)