##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920933_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3549407 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.640681105322663 31.0 30.0 34.0 25.0 34.0 2 29.794885173776915 31.0 30.0 34.0 25.0 34.0 3 29.876322157475883 31.0 30.0 34.0 25.0 34.0 4 33.32837288031494 37.0 35.0 37.0 27.0 37.0 5 33.36737094393514 37.0 35.0 37.0 27.0 37.0 6 33.44128498084328 37.0 35.0 37.0 27.0 37.0 7 33.397828425987775 37.0 35.0 37.0 27.0 37.0 8 33.34084566802286 37.0 35.0 37.0 27.0 37.0 9 34.76931498698233 39.0 35.0 39.0 27.0 39.0 10-11 34.76111530743023 39.0 35.0 39.0 27.0 39.0 12-13 34.69871361610545 39.0 35.0 39.0 27.0 39.0 14-15 35.86259549834662 40.0 36.0 41.0 27.0 41.0 16-17 35.84736126344485 40.0 36.0 41.0 27.0 41.0 18-19 35.827660226060296 40.0 36.0 41.0 27.0 41.0 20-21 35.84530401838955 40.0 36.0 41.0 27.0 41.0 22-23 35.77632348164073 40.0 36.0 41.0 27.0 41.0 24-25 35.77795319049069 40.0 36.0 41.0 27.0 41.0 26-27 35.70795896328598 40.0 36.0 41.0 27.0 41.0 28-29 35.60675797393762 40.0 35.5 41.0 26.0 41.0 30-31 35.460541859527524 40.0 35.0 41.0 25.5 41.0 32-33 35.33112362149508 40.0 35.0 41.0 25.0 41.0 34-35 35.26567705535037 40.0 35.0 41.0 25.0 41.0 36-37 35.13379079942086 40.0 35.0 41.0 24.5 41.0 38-39 34.992798797094835 39.0 35.0 41.0 24.0 41.0 40-41 34.85523342349863 39.0 34.5 41.0 24.0 41.0 42-43 34.670975461534844 39.0 34.0 41.0 23.0 41.0 44-45 34.51517605616938 39.0 34.0 41.0 23.0 41.0 46-47 34.31051975724395 39.0 33.5 41.0 22.0 41.0 48-49 34.18404158779199 39.0 33.0 41.0 20.5 41.0 50-51 33.981114450949136 38.0 33.0 40.0 19.5 41.0 52-53 33.7740957855777 38.0 33.0 40.0 18.5 41.0 54-55 33.52968524037959 38.0 33.0 40.0 17.5 41.0 56-57 33.25758049161452 38.0 32.5 40.0 14.5 41.0 58-59 32.98440922666801 37.0 32.0 40.0 13.0 41.0 60-61 32.703918710928335 37.0 32.0 40.0 10.5 41.0 62-63 32.387672645036204 36.0 31.0 40.0 8.0 41.0 64-65 32.087954833018586 36.0 31.0 39.0 7.5 41.0 66-67 31.72803879633978 35.0 31.0 39.0 7.0 40.5 68-69 31.39355292306574 35.0 31.0 38.5 6.0 40.0 70-71 31.04747440910552 35.0 30.0 37.5 2.0 40.0 72-73 31.10676713039671 35.0 31.0 37.0 2.0 39.5 74-75 31.096150145644046 35.0 31.0 37.0 2.0 39.0 76-77 30.836682859981963 35.0 31.0 36.5 2.0 39.0 78-79 30.54784433005288 35.0 31.0 36.0 2.0 39.0 80-81 30.290694755490144 35.0 31.0 36.0 2.0 37.0 82-83 30.054635464459274 35.0 31.0 35.0 2.0 37.0 84-85 29.826336483812646 35.0 31.0 35.0 2.0 36.5 86-87 29.640824086953117 35.0 30.5 35.0 2.0 36.0 88-89 29.45032592768313 35.0 30.0 35.0 2.0 36.0 90-91 29.287790185797235 34.5 30.0 35.0 2.0 36.0 92-93 29.137670320704274 34.0 30.0 35.0 2.0 35.0 94-95 29.030956720376107 34.0 30.0 35.0 2.0 35.0 96-97 28.942436018185575 34.0 30.0 35.0 2.0 35.0 98-99 28.81582641832847 34.0 30.0 35.0 2.0 35.0 100-101 28.699642503663288 34.0 29.0 35.0 2.0 35.0 102-103 27.337858267592303 32.5 26.0 34.5 2.0 35.0 104-105 28.362220646998217 34.0 29.0 35.0 2.0 35.0 106-107 28.520142096975636 34.0 29.0 35.0 2.0 35.0 108-109 28.458307824377425 34.0 29.0 35.0 2.0 35.0 110-111 28.35999055616896 34.0 29.0 35.0 2.0 35.0 112-113 28.245198000680112 34.0 29.0 35.0 2.0 35.0 114-115 28.131888228089935 34.0 29.0 35.0 2.0 35.0 116-117 27.922590027010145 34.0 29.0 35.0 2.0 35.0 118-119 27.724018406454938 34.0 29.0 35.0 2.0 35.0 120-121 27.547612601203525 34.0 28.5 35.0 2.0 35.0 122-123 27.261919667144397 34.0 27.0 35.0 2.0 35.0 124-125 25.93351734529176 32.5 23.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127838.0 3 18894.0 4 12829.0 5 12295.0 6 15494.0 7 16569.0 8 16383.0 9 17511.0 10 18868.0 11 20273.0 12 20875.0 13 20202.0 14 20388.0 15 18834.0 16 15644.0 17 14593.0 18 13976.0 19 14142.0 20 14592.0 21 15621.0 22 18457.0 23 23437.0 24 31645.0 25 40084.0 26 45124.0 27 50250.0 28 58411.0 29 69627.0 30 82239.0 31 100821.0 32 125275.0 33 161105.0 34 218123.0 35 321484.0 36 577329.0 37 887415.0 38 292346.0 39 414.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.808564437884684 18.056492328184927 11.657969967461458 25.47697326646893 2 16.401341635038705 20.37278110515606 38.234256835984894 24.991620423820343 3 19.32387572489157 24.365641388461732 29.03923593345602 27.27124695319068 4 12.971391023020406 16.295529510545858 35.81052467048511 34.92255479594862 5 13.96940776542586 37.73018747430211 34.16813904812056 14.13226571215147 6 33.388809120704536 35.828954074114186 17.004696686767122 13.777540118414153 7 28.36288267392577 32.21785324679867 21.237027978615274 18.18223610066029 8 26.25462972624056 35.29200762752157 19.795629346669276 18.657733299568598 9 26.337365346906306 15.802633541942162 19.19096887619431 38.66903223495722 10-11 25.316500670123922 26.21766437069682 27.506930347435215 20.958904611744046 12-13 25.599799027306823 23.87609447013541 27.252128391889986 23.271978110667778 14-15 23.062813806823566 25.197109029967564 24.57927893789501 27.160798225313865 16-17 23.36292355319473 27.692949661382997 25.541181014892384 23.40294577052989 18-19 23.386188087289074 26.96267697174838 26.710524256888174 22.94061068407437 20-21 24.379382250125843 26.30959158884262 26.263905743659414 23.047120417372124 22-23 23.802413250254027 26.198369018655633 26.147641346477897 23.851576384612446 24-25 23.58521775742128 26.30046017784936 26.45594833468674 23.65837373004262 26-27 23.429532764349666 26.36591251872612 26.414462633821188 23.790092083103023 28-29 23.517497028841 26.784665365949103 26.28733190274136 23.41050570246854 30-31 23.66647679514921 26.58867979528834 26.124939405331425 23.61990400423102 32-33 23.408226210425305 26.558525710978127 26.08855562649544 23.944692452101126 34-35 23.64529068427437 26.58010830485215 26.227352790051683 23.547248220821796 36-37 23.764245690979802 26.348938864833194 26.187204351339293 23.699611092847704 38-39 23.513984486053292 26.606257904562785 26.20018257672254 23.679575032661386 40-41 23.689514381103436 26.438790529387624 26.385249678973405 23.486445410535538 42-43 23.588291367560625 26.525430251726974 26.158087006977237 23.728191373735168 44-45 23.541918311198483 26.460865666116966 26.29014794830739 23.707068074377162 46-47 23.59942201803438 26.55504621360778 26.124378888575805 23.721152879782036 48-49 23.39062363457489 26.600990012487912 26.126435193929126 23.88195115900807 50-51 23.3084195800253 26.76095130365531 26.21819091617068 23.712438200148707 52-53 23.598221508537737 26.560151512610002 26.02227103430671 23.81935594454555 54-55 23.464391162272623 26.472219222911264 26.13401441341982 23.92937520139629 56-57 23.336069459545293 26.71233079469877 25.992677443987294 23.95892230176864 58-59 23.362689364441902 26.43786317711253 26.216082621044475 23.983364837401094 60-61 23.28441599155585 26.464997045450446 26.384973296284937 23.865613666708768 62-63 23.22660689072496 26.384196869318217 26.438083736216246 23.951112503740575 64-65 23.241196468439213 26.479475339963464 26.231803779627 24.047524411970322 66-67 23.10533480638313 26.596789830205548 26.121891957492043 24.17598340591928 68-69 23.45217404787176 26.267533269338927 26.25904766737568 24.02124501541363 70-71 23.257200840120916 26.18001934685546 26.369203008502275 24.193576804521353 72-73 23.25560122744696 26.18058331355429 26.385190693457922 24.178624765540828 74-75 23.081164529964223 26.386763509274598 26.354218597225493 24.17785336353569 76-77 23.152592841518395 26.196423381061617 26.42640043551156 24.22458334190843 78-79 23.17868827780857 26.35976428309258 26.347853836122397 24.113693602976454 80-81 23.266382230190576 26.19438653761313 26.40652991727663 24.132701314919665 82-83 23.20678382567443 26.111749485932854 26.588900209235334 24.092566479157377 84-85 23.17169778501487 26.196923338888016 26.373275837522836 24.258103038574273 86-87 23.09888841655175 26.11000481483194 26.517968794927626 24.27313797368869 88-89 23.200267462288043 26.02990458406908 26.32366770629664 24.44616024734624 90-91 23.003858983730474 26.11934912878981 26.48991192473614 24.386879962743567 92-93 23.274309129571407 25.912759113849066 26.576973442843332 24.235958313736198 94-95 23.111260338179736 25.838577659298252 26.64131685934155 24.40884514318046 96-97 23.087026206688158 25.71981574250404 26.635761649139546 24.557396401668264 98-99 23.023541262145844 25.933413376603117 26.45033091488781 24.592714446363235 100-101 22.984370375590164 25.874667042491723 26.61243041364245 24.528532168275667 102-103 22.863987302623485 25.836827380874368 26.658346062983192 24.640839253518955 104-105 22.79786407958544 25.9572956846121 26.514613636011315 24.730226599791145 106-107 22.681121161065214 26.106010963774217 26.4679417990298 24.744926076130774 108-109 22.640167802772545 26.099479125851516 26.549007215789832 24.711345855586107 110-111 22.64875710670255 26.244553025673987 26.44548233252778 24.661207535095674 112-113 22.72994060474401 26.362427489665947 26.323425478814173 24.58420642677587 114-115 22.737795151171216 26.488922981636293 26.198749346841506 24.57453252035099 116-117 22.5664357380368 26.3719865674501 26.465134250084333 24.59644344442877 118-119 22.730974675750833 26.395805001702715 26.203460010960534 24.669760311585918 120-121 22.677110415581613 26.553527619576535 26.166820423947236 24.60254154089462 122-123 22.723793774768797 26.60650082062275 26.028696142932127 24.641009261676324 124-125 22.849311507615138 26.54525416654156 25.984386720127183 24.62104760571612 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2006.0 1 1502.0 2 822.5 3 589.5 4 470.0 5 377.5 6 366.0 7 409.5 8 466.5 9 505.0 10 486.0 11 482.5 12 541.5 13 615.0 14 685.0 15 782.0 16 906.5 17 1100.0 18 1330.0 19 1573.5 20 1990.0 21 2674.5 22 3527.0 23 4618.5 24 6218.5 25 8205.5 26 11119.0 27 15248.0 28 18807.5 29 22724.5 30 27315.5 31 32842.5 32 40875.0 33 46776.0 34 50851.5 35 56618.5 36 64237.0 37 72225.0 38 80126.0 39 86519.5 40 93002.5 41 100812.0 42 105884.0 43 113260.5 44 121749.5 45 128683.0 46 134686.5 47 137788.0 48 141425.5 49 145147.5 50 147430.5 51 148830.5 52 148533.5 53 147067.0 54 143695.0 55 134683.5 56 123714.5 57 113773.0 58 104749.5 59 94491.5 60 80152.5 61 65911.0 62 52508.0 63 38899.5 64 28294.0 65 21522.0 66 16292.5 67 12332.0 68 9020.5 69 6386.0 70 4833.0 71 3548.5 72 2578.5 73 2194.5 74 1872.0 75 1481.0 76 1054.0 77 734.0 78 503.5 79 319.0 80 220.5 81 160.0 82 128.5 83 98.0 84 72.0 85 64.5 86 53.0 87 40.5 88 32.5 89 26.5 90 18.0 91 15.5 92 17.0 93 10.5 94 8.5 95 9.5 96 7.0 97 7.5 98 8.0 99 8.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16670390293364495 2 0.14298163045263618 3 0.16752094082194577 4 0.14171381303975567 5 0.16419644182817017 6 0.10469354458364453 7 0.10421459133877857 8 0.09300145066485754 9 0.09962227493212246 10-11 0.08587349943244041 12-13 0.0748434879403799 14-15 0.07435044783537081 16-17 0.074012363191936 18-19 0.07180072614946666 20-21 0.06639137185450977 22-23 0.05729126020205629 24-25 0.06174270800728121 26-27 0.07202611591175653 28-29 0.06734927834424173 30-31 0.0639966056301799 32-33 0.0728431538000573 34-35 0.06971587084828536 36-37 0.07170211812846485 38-39 0.06775779728839211 40-41 0.0727586326391986 42-43 0.07291358810077289 44-45 0.06546163908506407 46-47 0.06303869914044798 48-49 0.05569944500588408 50-51 0.061122886160984075 52-53 0.06320774146216537 54-55 0.06468686177719264 56-57 0.06822266367311497 58-59 0.055826226747172136 60-61 0.06578563686835576 62-63 0.060869322678407965 64-65 0.05541770780302175 66-67 0.05967193956624303 68-69 0.06260200647601134 70-71 0.0580519506497846 72-73 0.05299476785840565 74-75 0.05638970115289681 76-77 0.06519398874234485 78-79 0.05919298632137707 80-81 0.057530736824489274 82-83 0.062123053231145364 84-85 0.06339087064402589 86-87 0.05733352078248564 88-89 0.05609387708989136 90-91 0.05916481260109083 92-93 0.05374137144599084 94-95 0.06310913344116355 96-97 0.04967026886463006 98-99 0.04909270759876227 100-101 0.04668385451428929 102-103 0.04472578095439605 104-105 0.04288040227564773 106-107 0.05369911086556149 108-109 0.05378363202642018 110-111 0.06027767455239706 112-113 0.06068619349654745 114-115 0.062559745895582 116-117 0.0662505032530786 118-119 0.06015089281110901 120-121 0.0605594117552594 122-123 0.05919298632137707 124-125 0.0701666503728651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3549407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.619198363890646 #Duplication Level Percentage of deduplicated Percentage of total 1 69.54715319629393 32.42232530501918 2 14.200989818711877 13.240775226442409 3 6.106315976826647 8.540146673888286 4 3.107533419396177 5.79482867604999 5 1.854439368363944 4.322623838378339 6 1.2138322378998188 3.3952731527482176 7 0.8233033107554484 2.68672182504293 8 0.5854387992038088 2.1834150008000246 9 0.4235403484364234 1.7770600366982086 >10 1.9621677271513114 15.833742241981255 >50 0.11558926431829855 3.708269203494382 >100 0.05587039238852668 4.5246108712885675 >500 0.0026114925595169553 0.8309001742396744 >1k 0.0012146476942852002 0.7393077739285328 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.634744057246746E-5 2 0.0 0.0 0.0 2.817372028623373E-5 5.634744057246746E-5 3 0.0 0.0 0.0 5.634744057246746E-5 5.634744057246746E-5 4 0.0 0.0 0.0 1.1269488114493492E-4 5.634744057246746E-5 5 0.0 0.0 0.0 1.1269488114493492E-4 8.452116085870118E-5 6 2.817372028623373E-5 0.0 0.0 1.1269488114493492E-4 1.4086860143116864E-4 7 2.817372028623373E-5 0.0 0.0 1.972160420036361E-4 1.4086860143116864E-4 8 2.817372028623373E-5 0.0 0.0 2.817372028623373E-4 1.4086860143116864E-4 9 2.817372028623373E-5 0.0 0.0 2.817372028623373E-4 1.4086860143116864E-4 10-11 8.452116085870118E-5 0.0 0.0 2.817372028623373E-4 1.6904232171740236E-4 12-13 8.452116085870118E-5 0.0 0.0 3.239977832916879E-4 6.479955665833758E-4 14-15 8.452116085870118E-5 0.0 0.0 3.5217150357792163E-4 8.592984687301287E-4 16-17 1.2678174128805177E-4 0.0 0.0 3.662583637210385E-4 0.001056514510733765 18-19 1.6904232171740236E-4 0.0 0.0 4.366926644366228E-4 0.0011551225317355828 20-21 1.6904232171740236E-4 0.0 0.0 5.21213825295324E-4 0.0013241648534529852 22-23 1.972160420036361E-4 0.0 0.0 6.902561470127264E-4 0.0014368597345979202 24-25 1.972160420036361E-4 0.0 0.0 0.0010706013708768817 0.001450946594741037 26-27 2.1130290214675296E-4 0.0 0.0 0.0016481626367446732 0.0015918151961722055 28-29 2.2538976228986984E-4 0.0 0.0 0.004155623742219475 0.0016059020563153226 30-31 3.3808464343480473E-4 0.0 0.0 0.013424777716390373 0.0016059020563153226 32-33 3.3808464343480473E-4 0.0 0.0 0.03625957800838281 0.0016763363570309069 34-35 3.3808464343480473E-4 0.0 0.0 0.07256141659719496 0.0018876392591776598 36-37 3.3808464343480473E-4 0.0 0.0 0.13661436966794735 0.0018876392591776598 38-39 3.3808464343480473E-4 0.0 0.0 0.23895540860769138 0.0019439866997501272 40-41 3.3808464343480473E-4 0.0 0.0 0.35814433227860315 0.0019439866997501272 42-43 3.3808464343480473E-4 0.0 0.0 0.47623448085835185 0.0020285078606088287 44-45 3.5217150357792163E-4 0.0 0.0 0.6206952316260153 0.0021271158816106466 46-47 3.662583637210385E-4 0.0 0.0 0.7957667294846716 0.002267984483041815 48-49 3.662583637210385E-4 0.0 0.0 0.9818964125556748 0.0023947662243298667 50-51 3.662583637210385E-4 0.0 0.0 1.1784644589927273 0.0024792873851885683 52-53 3.944320840072722E-4 0.0 0.0 1.379089521150998 0.0026483297069059704 54-55 3.944320840072722E-4 0.0 0.0 1.6026479916222627 0.002789198308337139 56-57 3.944320840072722E-4 0.0 0.0 1.8743694369228439 0.0028314588887664895 58-59 3.944320840072722E-4 0.0 0.0 2.1690947248371346 0.002930066909768308 60-61 3.944320840072722E-4 0.0 0.0 2.4812172850281753 0.003000501210483892 62-63 3.944320840072722E-4 0.0 0.0 2.8056095004038704 0.0031272829517719437 64-65 3.944320840072722E-4 0.0 0.0 3.149103498133632 0.0031554566720581774 66-67 3.944320840072722E-4 0.0 0.0 3.5518750033456294 0.0031695435322012947 68-69 3.944320840072722E-4 0.0 0.0 4.002541833044224 0.0031836303923444115 70-71 4.226058042935059E-4 0.0 0.0 4.493313953570272 0.003225890972773762 72-73 4.226058042935059E-4 0.0 0.0 4.988579782481976 0.0034371938749205152 74-75 4.226058042935059E-4 0.0 0.0 5.493917716396006 0.003648496777067268 76-77 4.507795245797397E-4 0.0 0.0 6.057927422806119 0.0036907573574966185 78-79 4.507795245797397E-4 0.0 0.0 6.679425605460292 0.003747104798069086 80-81 4.507795245797397E-4 0.0 0.0 7.347297731705606 0.00381753909878467 82-83 4.507795245797397E-4 0.0 0.0 8.041399591537402 0.004000668280645189 84-85 4.507795245797397E-4 0.0 0.0 8.725936473332023 0.004000668280645189 86-87 4.507795245797397E-4 0.0 0.0 9.455311830962186 0.004113363161790125 88-89 4.507795245797397E-4 0.0 0.0 10.245781901033045 0.00422605804293506 90-91 4.7895324486597337E-4 0.0 0.0 11.090204645452044 0.004240144903078176 92-93 4.7895324486597337E-4 0.0 0.0 11.947333737720133 0.0045077952457973965 94-95 4.7895324486597337E-4 0.0 0.0 12.802997796533337 0.0045500558262267475 96-97 5.071269651522071E-4 0.0 0.0 13.699386968020292 0.0049163141899477856 98-99 5.634744057246746E-4 0.0 0.0 14.645361887211019 0.005127617092094539 100-101 5.634744057246746E-4 0.0 0.0 15.653642988814752 0.005127617092094539 102-103 5.634744057246746E-4 0.0 0.0 16.68792843424268 0.00521213825295324 104-105 5.634744057246746E-4 0.0 0.0 17.702506362330382 0.005296659413811941 106-107 5.634744057246746E-4 0.0 0.0 18.721338522181313 0.005296659413811941 108-109 5.634744057246746E-4 0.0 0.0 19.78260030478331 0.005324833134098175 110-111 5.634744057246746E-4 0.0 0.0 20.887010140003667 0.005353006854384408 112-113 5.634744057246746E-4 2.817372028623373E-5 0.0 22.020875599783288 0.005353006854384408 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7090 0.0 37.142624 1 GGTATCA 4690 0.0 31.631735 1 TCAACGC 7940 0.0 31.576355 4 CAACGCA 8120 0.0 30.949726 5 AACGCAG 8265 0.0 30.531473 6 ATCAACG 8275 0.0 30.4424 3 TATCAAC 8525 0.0 30.31852 2 CATGGGG 8500 0.0 29.706215 4 GTACATG 13440 0.0 29.479418 1 ACATGGG 12930 0.0 28.740387 3 TACATGG 13600 0.0 27.850363 2 ACGCAGA 9765 0.0 25.840082 7 CGCAGAG 9805 0.0 25.491167 8 GCGTTAG 205 2.2392069E-6 23.250519 1 GCAGAGT 11060 0.0 21.145565 9 ATGGGGA 5115 0.0 20.840483 5 AGAGTAC 9735 0.0 19.16172 10-11 ATGGGGC 3405 0.0 18.714018 5 GTATATA 1545 0.0 18.124495 1 CAGAGTA 10350 0.0 17.53446 10-11 >>END_MODULE