FastQCFastQC Report
Mon 15 Aug 2016
SRR2920933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549407
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66920.1885385361554761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.14948975983875618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37690.10618675175881492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56650.050.015541
GTATCAA94400.046.0013581
TCAACGC116900.036.8791924
ATCAACG118900.036.15923
TATCAAC121350.035.52732
CAACGCA121150.035.487155
AACGCAG124850.034.228286
GTACATG125800.029.8250581
CATGGGG84000.028.9935474
ACGCAGA147400.028.7435137
ACATGGG121550.028.561243
TACATGG129750.028.1789252
CGCAGAG150350.028.0964228
GCAGAGT165850.025.0777449
AGAGTAC149600.022.5715310-11
ATGGGGA48200.022.484425
GAGTACT98250.021.77173612-13
AGTACTT106950.021.44718612-13
CAGAGTA157800.021.30434610-11
GTACTTT108850.019.89769414-15
Produced by FastQC (version 0.11.5)