FastQCFastQC Report
Mon 15 Aug 2016
SRR2920932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728262
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36130.1324286303881372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG128250.028.5760251
CATGGGG81100.028.421234
TACATGG131900.026.9586582
GTATCAA85550.026.2606451
ACATGGG136000.025.4452043
GGTATCA68450.024.1151711
GTATAGG4950.022.8734911
AACGCAG98800.022.1703536
TATCAAC101150.022.081782
TCAACGC100150.021.8256844
CAACGCA102150.021.4566615
ATCAACG104350.021.0050663
ATGGGGC37550.020.937115
ATGGGGA40750.020.7545575
ACGCAGA110150.019.6679367
GTATATA8800.019.6380961
CGCAGAG111700.019.2873868
TAAGGTA6200.019.2128164
CCGTGCG3503.4011464E-818.7043519
GTATTAC6950.018.0060541
Produced by FastQC (version 0.11.5)