FastQCFastQC Report
Mon 15 Aug 2016
SRR2920932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728262
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81940.3003377241628553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66260.242865238015997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48100.17630271579489068No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT29490.10809079186676353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77600.042.2774931
GTATCAA118200.040.4729771
TATCAAC150500.031.7720762
TCAACGC150000.031.439574
ATCAACG152500.031.0803493
CAACGCA152000.030.9867175
AACGCAG155500.030.3125426
GTACATG131200.027.0060371
CATGGGG81400.026.8462924
ACGCAGA175500.026.5814657
TACATGG134800.025.8865572
CGCAGAG180650.025.7220338
GAGTACT102300.024.16637412-13
ACATGGG140200.024.166123
AGTACTT106300.023.7607712-13
GCAGAGT190550.023.7312589
AGAGTAC175000.021.72595610-11
GTACTTT114000.021.58195314-15
TACTTTT121350.020.6670214-15
CAGAGTA183500.020.63851510-11
Produced by FastQC (version 0.11.5)