FastQCFastQC Report
Mon 15 Aug 2016
SRR2920931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666615
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62550.031.6308291
GTACATG103350.030.7914811
TACATGG103100.029.6378272
CATGGGG70200.028.3376834
ACATGGG109800.027.6665783
TCAACGC70350.027.4306374
CAACGCA72150.026.5811985
TATCAAC74450.026.0819782
AACGCAG73650.026.0212556
GGTATCA46100.025.9833371
ATCAACG75150.025.601253
ACGCAGA81850.023.2667477
CGCAGAG82850.022.9115058
GTCCTAA10050.021.346971
GTATAAT12850.020.8693621
ATGGGGA37200.020.3336725
ATGGGGG32850.020.306625
GCAGAGT93850.019.592049
TACACCG3753.5815901E-919.0592145
GTATAGG8800.018.2844091
Produced by FastQC (version 0.11.5)