FastQCFastQC Report
Mon 15 Aug 2016
SRR2920931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666615
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55380.20767902378108577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45850.17194083135360747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32800.12300238317117394No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT27400.10275199081982214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90350.040.7407881
GGTATCA58600.039.399051
TCAACGC110550.033.070564
CAACGCA111200.032.8237045
TATCAAC111000.032.7761762
ATCAACG112200.032.531163
AACGCAG114400.031.8379756
ACGCAGA129700.028.1212487
CGCAGAG131150.027.5803978
GTACATG103950.026.8018511
CATGGGG66250.026.6034374
TACATGG105300.025.6724622
ACATGGG106250.024.8820363
GCAGAGT144100.024.2801789
GAGTACT78400.022.92078212-13
AGTACTT80700.022.00945712-13
AGAGTAC130250.021.44847310-11
GTACTTT83550.021.36551314-15
CAGAGTA136800.020.70424510-11
TTAGCGC2801.5569076E-619.1388744
Produced by FastQC (version 0.11.5)