FastQCFastQC Report
Mon 15 Aug 2016
SRR2920930_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920930_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4396224
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109800.030.0691221
GTACATG170150.027.7400071
TACATGG170200.026.5630422
TCAACGC120000.026.5054864
CAACGCA121000.026.3356575
CATGGGG104550.026.3204754
AACGCAG121550.026.0528586
ATCAACG124150.025.6686253
TATCAAC127550.025.3112962
ACATGGG178550.024.9871943
ACGCAGA137650.022.6995647
GGTATCA83450.021.9242821
CGCAGAG141450.021.5826288
GTATAGG10150.020.5501671
GCAGAGT159500.018.2066179
GTATAAT18700.017.8467751
GTATACG3053.4967343E-617.5855541
ATGGGGG52600.017.5518025
GTATTAC12250.017.5137751
GTATAGA16300.016.8183571
Produced by FastQC (version 0.11.5)