##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920930_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4396224
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.659404525338108	31.0	30.0	34.0	25.0	34.0
2	29.83110824198221	31.0	30.0	34.0	25.0	34.0
3	30.024796734652284	31.0	31.0	34.0	25.0	34.0
4	33.42668981380385	37.0	35.0	37.0	27.0	37.0
5	33.525430915258184	37.0	35.0	37.0	28.0	37.0
6	33.496141916335475	37.0	35.0	37.0	28.0	37.0
7	33.3621969672155	37.0	35.0	37.0	27.0	37.0
8	33.35274339979036	37.0	35.0	37.0	27.0	37.0
9	34.784022379205425	39.0	35.0	39.0	27.0	39.0
10-11	34.78397574827852	39.0	35.0	39.0	27.0	39.0
12-13	34.732938881185305	39.0	35.0	39.0	27.0	39.0
14-15	35.90523776768427	40.0	36.0	41.0	27.0	41.0
16-17	35.89641576498377	40.0	36.0	41.0	27.0	41.0
18-19	35.87203552412252	40.0	36.0	41.0	27.0	41.0
20-21	35.887988419152435	40.0	36.0	41.0	27.0	41.0
22-23	35.82229226718202	40.0	36.0	41.0	27.0	41.0
24-25	35.82721490078758	40.0	36.0	41.0	27.0	41.0
26-27	35.757867092304664	40.0	36.0	41.0	27.0	41.0
28-29	35.65542986435632	40.0	36.0	41.0	26.5	41.0
30-31	35.513272299136716	40.0	35.0	41.0	25.5	41.0
32-33	35.37989840827037	40.0	35.0	41.0	25.0	41.0
34-35	35.325807897868714	40.0	35.0	41.0	25.0	41.0
36-37	35.19600252398422	40.0	35.0	41.0	24.5	41.0
38-39	35.050749575089895	40.0	35.0	41.0	24.0	41.0
40-41	34.91734497605218	39.0	35.0	41.0	24.0	41.0
42-43	34.73707151864873	39.0	34.5	41.0	23.0	41.0
44-45	34.583307174520684	39.0	34.0	41.0	22.5	41.0
46-47	34.382107235664066	39.0	34.0	41.0	21.5	41.0
48-49	34.260288261016726	39.0	34.0	41.0	20.5	41.0
50-51	34.06303716553115	38.5	33.0	40.5	19.5	41.0
52-53	33.86654774642966	38.0	33.0	40.0	18.5	41.0
54-55	33.62484657287709	38.0	33.0	40.0	17.5	41.0
56-57	33.358151563705576	38.0	33.0	40.0	14.5	41.0
58-59	33.0915159236654	37.5	32.0	40.0	13.0	41.0
60-61	32.82585088930864	37.0	32.0	40.0	10.5	41.0
62-63	32.51415316871934	36.5	32.0	40.0	8.0	41.0
64-65	32.22811349012243	36.0	31.0	39.0	7.5	41.0
66-67	31.879793886753724	35.5	31.0	39.0	7.0	41.0
68-69	31.548837024683003	35.0	31.0	39.0	6.5	40.0
70-71	31.207653659140206	35.0	31.0	38.0	2.0	40.0
72-73	31.27134888941055	35.0	31.0	37.5	2.0	40.0
74-75	31.258668120641715	35.0	31.0	37.0	2.0	39.0
76-77	30.988098991316186	35.0	31.0	37.0	2.0	39.0
78-79	30.699042291748555	35.0	31.0	36.0	2.0	39.0
80-81	30.43075841904325	35.0	31.0	36.0	2.0	37.5
82-83	30.19074016701606	35.0	31.0	35.5	2.0	37.0
84-85	29.96138356462273	35.0	31.0	35.0	2.0	37.0
86-87	29.769066134937617	35.0	31.0	35.0	2.0	36.0
88-89	29.58291615713849	35.0	31.0	35.0	2.0	36.0
90-91	29.423611262756403	35.0	30.0	35.0	2.0	36.0
92-93	29.26965812024137	35.0	30.0	35.0	2.0	35.0
94-95	29.165766234841534	34.5	30.0	35.0	2.0	35.0
96-97	29.077906062111484	34.0	30.0	35.0	2.0	35.0
98-99	28.95824791912332	34.0	30.0	35.0	2.0	35.0
100-101	28.846644756955058	34.0	30.0	35.0	2.0	35.0
102-103	27.500728352331457	32.5	26.5	34.5	2.0	35.0
104-105	28.519398693060225	34.0	29.0	35.0	2.0	35.0
106-107	28.6742451021604	34.0	29.5	35.0	2.0	35.0
108-109	28.61553437677425	34.0	29.5	35.0	2.0	35.0
110-111	28.52954228446958	34.0	29.0	35.0	2.0	35.0
112-113	28.425673259597325	34.0	29.0	35.0	2.0	35.0
114-115	28.323987130774043	34.0	29.0	35.0	2.0	35.0
116-117	28.125064714627825	34.0	29.0	35.0	2.0	35.0
118-119	27.94619120863723	34.0	29.0	35.0	2.0	35.0
120-121	27.787625926249433	34.0	29.0	35.0	2.0	35.0
122-123	27.516172629056207	34.0	29.0	35.0	2.0	35.0
124-125	26.21895517607838	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	166075.0
3	23697.0
4	15696.0
5	14477.0
6	18930.0
7	20589.0
8	20226.0
9	20932.0
10	22837.0
11	24273.0
12	24179.0
13	23712.0
14	23805.0
15	21570.0
16	18376.0
17	16982.0
18	16251.0
19	16199.0
20	16834.0
21	18160.0
22	21621.0
23	27443.0
24	36823.0
25	46789.0
26	53290.0
27	59345.0
28	69661.0
29	82010.0
30	97477.0
31	119787.0
32	150455.0
33	193477.0
34	263383.0
35	390670.0
36	702382.0
37	1131974.0
38	405211.0
39	626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21095611806328	18.20639313853289	12.089252663427967	25.493398079975865
2	16.244587901411077	20.094348090143704	38.884120152119664	24.776943856325552
3	19.295809052366682	24.006745729914684	28.913965739056646	27.78347947866199
4	13.046594214569346	16.09476255031322	36.160638724155426	34.69800451096201
5	14.049447990426305	37.44017579725349	34.28349494564667	14.226881266673534
6	33.164608271004575	36.14117705998021	16.873477836618	13.820736832397214
7	28.72733284435074	32.06223666044166	20.998212200077834	18.212218295129766
8	25.94481316170733	35.71638858612558	19.787711753746	18.55108649842109
9	26.317059814035353	16.17179699409909	19.229067592353292	38.28207559951226
10-11	25.219305060884267	26.12565631576605	27.634264762985243	21.020773860364443
12-13	25.601252707744358	23.6357527088257	27.460155992027275	23.30283859140267
14-15	23.058677941631434	25.216510602377877	24.678589063184013	27.04622239280668
16-17	23.32552425042365	27.768073652301474	25.636480854353582	23.26992124292129
18-19	23.264324333799166	27.11673746721097	26.896186240526383	22.722751958463476
20-21	24.19572903067054	26.342105715419628	26.48675219652892	22.975413057380916
22-23	23.70486205987297	26.216983039902985	26.22006712843021	23.858087771793837
24-25	23.404841798673715	26.3577412648185	26.489202030534496	23.74821490597329
26-27	23.476765762973574	26.51054894150912	26.426367294451264	23.586318001066044
28-29	23.425252167594778	26.61045756141988	26.478567731994584	23.485722538990757
30-31	23.693437648452946	26.579380032035676	26.258476969207372	23.468705350304003
32-33	23.272021580522896	26.60531317937102	26.335715993034135	23.786949247071945
34-35	23.585031611053438	26.5869634324299	26.39850471357359	23.429500242943067
36-37	23.68751877306476	26.45428295628236	26.22363960086316	23.634558669789715
38-39	23.56724724883314	26.616490145149523	26.25669787590308	23.559564730114253
40-41	23.66475240458899	26.606042360375632	26.330472847963275	23.3987323870721
42-43	23.52830837456579	26.547194510387666	26.311836305056012	23.612660809990533
44-45	23.557598303382196	26.608180212605316	26.288539293924195	23.545682190088296
46-47	23.48125573456229	26.59726124930207	26.219676385746805	23.701806630388838
48-49	23.444791055463174	26.596177122732012	26.291703898395273	23.667327923409538
50-51	23.358106002685382	26.750078583480697	26.21155697262518	23.680258441208746
52-53	23.43307266198996	26.69454677431305	26.135905168180308	23.73647539551669
54-55	23.441449412298144	26.59891358494547	26.202362643747296	23.757274359009088
56-57	23.43582585513058	26.656204808402993	26.066689566208474	23.841279770257955
58-59	23.316826939599892	26.50570106729005	26.242753464032532	23.934718529077525
60-61	23.46621937065196	26.427537203408985	26.32164659441734	23.78459683152171
62-63	23.279439608752202	26.472548046452538	26.346996953747194	23.901015391048066
64-65	23.278420294309736	26.319214230269694	26.389792486044524	24.01257298937605
66-67	23.347447042295038	26.45592647745837	26.218597661422343	23.978028818824246
68-69	23.538287430116352	26.27730987619083	26.16811134221671	24.01629135147611
70-71	23.261368072096513	26.132994807166405	26.36723564402858	24.238401476708496
72-73	23.32280859233998	26.09824720160846	26.442006348073434	24.13693785797813
74-75	23.259229569856313	26.31455742868123	26.347559703675987	24.07865329778647
76-77	23.195916709412444	26.08784920132072	26.502704222857876	24.21352986640897
78-79	23.300369920843426	26.083192073758838	26.49147634321781	24.124961662179935
80-81	23.23713084778476	26.0944021385664	26.463689493632497	24.20477752001634
82-83	23.19143175576102	26.10938648566123	26.52185882618313	24.177322932394617
84-85	23.235187437980937	26.176081727965027	26.366189110639105	24.222541723414924
86-87	23.174808596333662	25.98392113609041	26.498898168560864	24.342372099015066
88-89	23.296474943483357	25.818519705003908	26.512401227462334	24.372604124050397
90-91	23.258533085740382	25.930228998442356	26.481839143562375	24.329398772254887
92-93	23.236995877299474	25.867464987543592	26.560294438291727	24.33524469686521
94-95	23.103453573840905	25.8220184039619	26.655448948666137	24.419079073531062
96-97	23.19198649318953	25.77752975894139	26.561383580968435	24.469100166900645
98-99	23.133271528661965	25.81642647089447	26.457398643885497	24.592903356558068
100-101	23.0826248548887	25.86399398640113	26.55728519601888	24.496095962691292
102-103	23.01844806660903	25.90890233059232	26.549451664827465	24.523197937971183
104-105	22.880763278867597	26.01195894588321	26.44632697646898	24.66095079878021
106-107	22.838741541169956	26.0139984284343	26.467578918259804	24.679681112135935
108-109	22.870635859568207	26.04342438934239	26.50651864280405	24.579421108285352
110-111	22.854483344767164	26.17380776684267	26.349878931869075	24.621829956521086
112-113	22.82915925970606	26.266595954376914	26.40659035505612	24.49765443086091
114-115	22.803759782113005	26.32035265626314	26.32745440116414	24.548433160459716
116-117	22.72817909700329	26.326235161558337	26.33051455106245	24.615071190375925
118-119	22.81520477246865	26.268994328943577	26.28724877798186	24.628552120605914
120-121	22.793596476892354	26.342618030615967	26.31303953162407	24.550745960867612
122-123	22.816309074223117	26.419793438240873	26.179083163687082	24.584814323848924
124-125	22.86943292132367	26.42498104077199	26.034335011124	24.671251026780343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1513.0
1	1194.0
2	671.0
3	437.5
4	366.5
5	318.0
6	313.0
7	326.0
8	375.5
9	420.0
10	429.0
11	437.5
12	490.5
13	586.5
14	679.5
15	763.5
16	932.5
17	1161.5
18	1390.5
19	1717.5
20	2174.5
21	2743.0
22	3578.5
23	4889.5
24	6499.5
25	8814.5
26	12570.5
27	17271.5
28	21874.5
29	27077.5
30	33323.0
31	40687.5
32	49506.5
33	57796.0
34	65433.0
35	73610.5
36	83733.5
37	94159.0
38	102132.0
39	109348.0
40	119075.5
41	128602.5
42	136259.0
43	146422.5
44	156380.5
45	164719.5
46	170964.0
47	176853.5
48	184658.0
49	190466.5
50	191936.5
51	186689.5
52	178156.5
53	172218.0
54	166325.0
55	157484.0
56	144706.5
57	130034.0
58	117691.0
59	105643.0
60	91024.0
61	76264.5
62	62500.5
63	47783.0
64	35262.0
65	27122.5
66	20996.5
67	16169.0
68	12633.0
69	10178.5
70	8497.5
71	6903.0
72	5536.5
73	4611.5
74	3736.5
75	2976.0
76	2297.0
77	1577.5
78	1065.5
79	741.5
80	493.0
81	388.0
82	286.0
83	181.0
84	148.5
85	116.0
86	79.0
87	64.5
88	54.0
89	44.0
90	36.0
91	27.0
92	21.5
93	20.0
94	16.5
95	14.5
96	14.0
97	10.0
98	10.5
99	12.0
100	21.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16889494256889548
2	0.14835458793728437
3	0.17446790700382872
4	0.1472399950502977
5	0.17132884948537655
6	0.11052667016057417
7	0.10902538178218397
8	0.09715155551673435
9	0.10302022826862325
10-11	0.0909303074638599
12-13	0.07960240424509761
14-15	0.07922708215050006
16-17	0.07906785459521626
18-19	0.07658845409151126
20-21	0.07050368680030863
22-23	0.06154145011719148
24-25	0.06592020788749618
26-27	0.07603115764801795
28-29	0.07195948159147487
30-31	0.06817214045508145
32-33	0.07674768164679506
34-35	0.07323330203374533
36-37	0.07493931155464326
38-39	0.07127707778311569
40-41	0.07555347498216651
42-43	0.07609939802885386
44-45	0.068536089152873
46-47	0.06612492903000393
48-49	0.05845925958276921
50-51	0.06385024966880669
52-53	0.06570411334818244
54-55	0.06750111004352827
56-57	0.07103823645018997
58-59	0.05852749996360514
60-61	0.06849059556564907
62-63	0.06354316795504505
64-65	0.05731054650536461
66-67	0.06236033468722249
68-69	0.0648283617941215
70-71	0.06071119215035448
72-73	0.055729644349332516
74-75	0.058857328470978736
76-77	0.0682972478199473
78-79	0.06179166484692318
80-81	0.05974445342184566
82-83	0.06456677366758382
84-85	0.06636377036292965
86-87	0.05982406719948757
88-89	0.05928951754960621
90-91	0.06192814560859501
92-93	0.05691247761715508
94-95	0.06543115182483877
96-97	0.05214702435544686
98-99	0.05171483527681938
100-101	0.04965625045493588
102-103	0.04788200055320202
104-105	0.04603951027063225
106-107	0.05724230612452869
108-109	0.057276426314946643
110-111	0.06437342592188205
112-113	0.06446441309632994
114-115	0.06681870623516908
116-117	0.06992364356320332
118-119	0.06311097887641758
120-121	0.06437342592188205
122-123	0.06296312471793976
124-125	0.07380197187404464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4396224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.49788606482046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.71055689371627	32.636062027880996
2	14.510732583295216	13.784582459168682
3	6.335122501857499	9.027147803997236
4	3.280176311585962	6.232057628809322
5	1.9249851492359102	4.571636264743935
6	1.1984259528684673	3.415361961988222
7	0.8420892931447784	2.799822291153712
8	0.5934407605757308	2.2549745305637168
9	0.43177229530226324	1.8457444159391625
>10	2.032164934625522	16.649950799263106
>50	0.10344436382978076	3.340978672873276
>100	0.035791121268853504	2.848180739624183
>500	9.613620643791388E-4	0.3132458702125885
>1k	3.3647662939970336E-4	0.2802545337817951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.2746793611972456E-5	0.0
3	0.0	0.0	0.0	2.2746793611972456E-5	0.0
4	0.0	0.0	0.0	2.2746793611972456E-5	0.0
5	0.0	0.0	0.0	2.2746793611972456E-5	0.0
6	0.0	0.0	0.0	2.2746793611972456E-5	0.0
7	0.0	0.0	0.0	2.2746793611972456E-5	0.0
8	0.0	0.0	0.0	2.2746793611972456E-5	0.0
9	0.0	0.0	0.0	2.2746793611972456E-5	0.0
10-11	0.0	0.0	0.0	2.2746793611972456E-5	0.0
12-13	0.0	0.0	0.0	2.2746793611972456E-5	0.0
14-15	0.0	0.0	0.0	2.2746793611972456E-5	0.0
16-17	0.0	0.0	0.0	2.2746793611972456E-5	0.0
18-19	0.0	0.0	0.0	6.824038083591736E-5	0.0
20-21	0.0	0.0	0.0	1.1373396805986228E-4	0.0
22-23	0.0	0.0	0.0	1.7060095208979342E-4	0.0
24-25	0.0	0.0	0.0	3.639486977915593E-4	0.0
26-27	0.0	0.0	0.0	7.733909828070635E-4	0.0
28-29	0.0	0.0	0.0	0.002627254662182819	0.0
30-31	0.0	0.0	0.0	0.009747001062730198	0.0
32-33	0.0	0.0	0.0	0.02888842788720502	0.0
34-35	2.2746793611972456E-5	0.0	0.0	0.061973639195818955	0.0
36-37	2.2746793611972456E-5	0.0	0.0	0.12186594677614243	0.0
38-39	2.2746793611972456E-5	0.0	0.0	0.2218494780975674	0.0
40-41	2.2746793611972456E-5	0.0	0.0	0.341884307987946	0.0
42-43	2.2746793611972456E-5	0.0	0.0	0.4558002503967041	0.0
44-45	2.2746793611972456E-5	0.0	0.0	0.5949082667307217	0.0
46-47	2.2746793611972456E-5	0.0	0.0	0.7674313228807268	0.0
48-49	2.2746793611972456E-5	0.0	0.0	0.9503041701241792	0.0
50-51	2.2746793611972456E-5	0.0	0.0	1.1355426839032772	0.0
52-53	2.2746793611972456E-5	0.0	0.0	1.324693646183634	0.0
54-55	2.2746793611972456E-5	0.0	0.0	1.5388774548339665	0.0
56-57	2.2746793611972456E-5	0.0	0.0	1.794437681064477	0.0
58-59	2.2746793611972456E-5	0.0	0.0	2.065306499395845	0.0
60-61	2.2746793611972456E-5	0.0	0.0	2.351154081320697	0.0
62-63	2.2746793611972456E-5	0.0	0.0	2.641835356888093	0.0
64-65	2.2746793611972456E-5	0.0	0.0	2.9553999068291334	0.0
66-67	2.2746793611972456E-5	0.0	0.0	3.3243301524217146	0.0
68-69	2.2746793611972456E-5	0.0	0.0	3.73246449680453	0.0
70-71	2.2746793611972456E-5	0.0	0.0	4.176117959412442	0.0
72-73	2.2746793611972456E-5	0.0	0.0	4.619270992560889	0.0
74-75	2.2746793611972456E-5	0.0	0.0	5.07060149801284	0.0
76-77	2.2746793611972456E-5	0.0	0.0	5.581733323870667	0.0
78-79	3.412019041795868E-5	0.0	0.0	6.145467109956181	0.0
80-81	4.549358722394491E-5	0.0	0.0	6.732891681588564	0.0
82-83	4.549358722394491E-5	0.0	0.0	7.340606393122825	0.0
84-85	4.549358722394491E-5	0.0	0.0	7.95678291188074	0.0
86-87	4.549358722394491E-5	0.0	0.0	8.611754087143876	0.0
88-89	4.549358722394491E-5	0.0	0.0	9.331690104962805	0.0
90-91	4.549358722394491E-5	0.0	0.0	10.085723566406081	0.0
92-93	6.824038083591736E-5	0.0	0.0	10.850834716338385	0.0
94-95	6.824038083591736E-5	0.0	0.0	11.62031325064419	0.0
96-97	6.824038083591736E-5	0.0	0.0	12.417167551062002	0.0
98-99	6.824038083591736E-5	0.0	0.0	13.273732184711243	0.0
100-101	6.824038083591736E-5	0.0	0.0	14.192463805302005	0.0
102-103	6.824038083591736E-5	0.0	0.0	15.117769249246626	0.0
104-105	6.824038083591736E-5	0.0	0.0	16.03910537770596	0.0
106-107	6.824038083591736E-5	0.0	0.0	16.969608463990916	0.0
108-109	6.824038083591736E-5	0.0	0.0	17.944399557438384	0.0
110-111	6.824038083591736E-5	2.2746793611972456E-5	0.0	18.96353552503239	0.0
112-113	6.824038083591736E-5	2.2746793611972456E-5	0.0	20.01582494431585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10980	0.0	30.069122	1
GTACATG	17015	0.0	27.740007	1
TACATGG	17020	0.0	26.563042	2
TCAACGC	12000	0.0	26.505486	4
CAACGCA	12100	0.0	26.335657	5
CATGGGG	10455	0.0	26.320475	4
AACGCAG	12155	0.0	26.052858	6
ATCAACG	12415	0.0	25.668625	3
TATCAAC	12755	0.0	25.311296	2
ACATGGG	17855	0.0	24.987194	3
ACGCAGA	13765	0.0	22.699564	7
GGTATCA	8345	0.0	21.924282	1
CGCAGAG	14145	0.0	21.582628	8
GTATAGG	1015	0.0	20.550167	1
GCAGAGT	15950	0.0	18.206617	9
GTATAAT	1870	0.0	17.846775	1
GTATACG	305	3.4967343E-6	17.585554	1
ATGGGGG	5260	0.0	17.551802	5
GTATTAC	1225	0.0	17.513775	1
GTATAGA	1630	0.0	16.818357	1
>>END_MODULE