FastQCFastQC Report
Mon 15 Aug 2016
SRR2920930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4396224
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68210.15515587922726415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54120.12310564702799494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128300.036.692941
GGTATCA88100.034.9466861
TCAACGC152800.030.201914
ATCAACG155450.029.6104373
CAACGCA155600.029.5053585
TATCAAC160700.029.0142842
AACGCAG161600.028.5781576
GTACATG159750.026.1823921
CATGGGG94700.025.7175394
TACATGG163250.025.278192
ACGCAGA183250.025.0664757
ACATGGG166050.024.600353
CGCAGAG189700.024.148718
GCAGAGT209150.021.3914599
GAGTACT108250.021.16156812-13
AGTACTT114900.020.92070812-13
AGAGTAC183800.020.58847410-11
GTACTTT117850.019.43786814-15
CAGAGTA200200.018.53040510-11
ATGGGGG50350.017.5032355
Produced by FastQC (version 0.11.5)